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Literature summary extracted from

  • Huang, X.P.; Kagami, N.; Inoue, H.; Kojima, M.; Kimura, T.; Makabe, O.; Suzuki, K.; Takahashi, K.
    Identification of a glutamic acid and an aspartic acid residue essential for catalytic activity of aspergillopepsin II, a non-pepsin type acid proteinase (2000), J. Biol. Chem., 275, 26607-26614.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.19 wild-type and mutant pro-enzymes expressed in Escherichia coli Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.4.23.19 D123A site-directed mutagenesis, activity is almost at background levels Aspergillus niger
3.4.23.19 D123E site-directed mutagenesis, activity not detectable, below 0.2% Aspergillus niger
3.4.23.19 D123E/E219D site-directed mutagenesis, weak activity against acid-denatured hemoglobin Aspergillus niger
3.4.23.19 D123N site-directed mutagenesis Aspergillus niger
3.4.23.19 D125N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
3.4.23.19 D137N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
3.4.23.19 D148N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
3.4.23.19 D160N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
3.4.23.19 D170N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
3.4.23.19 D208N site-directed mutagenesis Aspergillus niger
3.4.23.19 D220N site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
3.4.23.19 D225N site-directed mutagenesis Aspergillus niger
3.4.23.19 D254N site-directed mutagenesis Aspergillus niger
3.4.23.19 E128Q site-directed mutagenesis Aspergillus niger
3.4.23.19 E141Q site-directed mutagenesis Aspergillus niger
3.4.23.19 E152Q site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
3.4.23.19 E177Q site-directed mutagenesis Aspergillus niger
3.4.23.19 E189Q site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger
3.4.23.19 E206Q site-directed mutagenesis Aspergillus niger
3.4.23.19 E211Q site-directed mutagenesis Aspergillus niger
3.4.23.19 E2154A site-directed mutagenesis Aspergillus niger
3.4.23.19 E219A site-directed mutagenesis, activity is almost at background levels Aspergillus niger
3.4.23.19 E219D site-directed mutagenesis, activity not detectable, below 0.2% Aspergillus niger
3.4.23.19 E219Q site-directed mutagenesis Aspergillus niger
3.4.23.19 E222Q site-directed mutagenesis, markedly active toward acid-denatured hemoglobin Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.19 Aspergillus niger
-
 var.macrosporus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.19 Hemoglobin + H2O
-
 Aspergillus niger ?
-
 ?
3.4.23.19 Milk casein + H2O
-
 Aspergillus niger ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.23.19 acid proteinase A
-
 Aspergillus niger
3.4.23.19 non-pepsin type acid proteinase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.19 55 60
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.23.19 55 65 large decrease in activity at 60-65°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.19 2.6
-
 milk casein as substrate Aspergillus niger
3.4.23.19 5.3
-
 hemoglobin as substrate Aspergillus niger