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Literature summary extracted from

  • Creuzenet, C.; Belanger, M.; Wakarchuk, W.W.; Lam, J.S.
    Expression, purification, and biochemical characterization of WbpP, a new UDP-GlcNAc C4 epimerase from Pseudomonas aeruginosa serotype O6 (2000), J. Biol. Chem., 275, 19060-19067.
    View publication on PubMed

General Stability

EC Number General Stability Organism
5.1.3.7 2-mercaptoethanol stabilizes the enzyme increasing its lifetime in the reaction mixture by 50% Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.7 0.131
-
 UDP-N-acetyl-D-galactosamine pH 8.0, 37°C Pseudomonas aeruginosa
5.1.3.7 0.137
-
 UDP-N-acetyl-D-glucosamine pH 8.0, 37°C Pseudomonas aeruginosa
5.1.3.7 0.153
-
 UDP-D-glucose pH 8.0, 37°C Pseudomonas aeruginosa
5.1.3.7 0.16
-
 UDP-D-galactose pH 8.0, 37°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.7 additional information activity is independent of divalent metal Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.7 80000
-
 gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.7 Pseudomonas aeruginosa
-
 serotype O6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.7
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.7 UDP-D-glucose the achieved reasonable conversion rates the amount of enzyme used is increased 200fold comparted to UDP-N-acetyl-D-glucosamine as substrate. Substrate conversions reach 20% for UDP-Glc and 65% for UDP-Gal Pseudomonas aeruginosa UDP-D-galactose
-
 r
5.1.3.7 UDP-N-acetyl-D-glucosamine equilibrium reaction resulting in a 70:30 ratio of UDP-GlcNAc to UDP-GalNAc, irrespective of the initial substrate Pseudomonas aeruginosa UDP-N-acetyl-D-galactosamine
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.3.7 dimer
-
 Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
5.1.3.7 WbPP
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.7 37
-
-
 Pseudomonas aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.3.7 10 50 20% of maximal activity at 10°C and at 50°C Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.7 0.52
-
 UDP-D-glucose pH 8.0, 37°C Pseudomonas aeruginosa
5.1.3.7 3.4
-
 UDP-D-galactose pH 8.0, 37°C Pseudomonas aeruginosa
5.1.3.7 36.9
-
 UDP-D-galactose pH 8.0, 37°C Pseudomonas aeruginosa
5.1.3.7 27660
-
 UDP-N-acetyl-D-glucosamine pH 8.0, 37°C Pseudomonas aeruginosa
5.1.3.7 27700
-
 UDP-N-acetyl-D-glucosamine pH 8.0, 37°C Pseudomonas aeruginosa
5.1.3.7 62280
-
 UDP-N-acetyl-D-glucosamine pH 8.0, 37°C Pseudomonas aeruginosa
5.1.3.7 62300
-
 UDP-N-acetyl-D-glucosamine pH 8.0, 37°C Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.7 7 9
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.3.7 NAD+ tight binding to protein during its synthesis Pseudomonas aeruginosa
5.1.3.7 NADH tight binding to protein during its synthesis Pseudomonas aeruginosa