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Literature summary extracted from

  • Roghani, M.; Becherer, J.D.; Moss, M.L.; Atherton, R.E.; Erdjument-Bromage, H.; Arribas, J.; Blackburn, R.K.; Weskamp, G.; Tempst, P.; Blobel, C.P.
    Metalloprotease-disintegrin MDC9: intracellular maturation and catalytic activity (1999), J. Biol. Chem., 274, 3531-3540.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.B9 Protein kinase C phosphorylation of the enzyme by protein kinase C activates the enzyme, phosphorylation is stimualted by phorbol 12-myristate 13-acetate Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B9 expression of soluble enzyme form, nucleotides 1-1253, with a Myc-epitope in COS-7 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.B9 additional information site-directed mutagenesis of the furin cleavage site and of the cysteine-switch region results in highly reduced precursor processing, construction of a deletion mutant resulting in a soluble enzyme form Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B9 1,10-phenanthroline
-
 Mus musculus
3.4.24.B9 batimastat
-
 Mus musculus
3.4.24.B9 brefeldin A blocks processing of the enzyme precursor by blocking the vesicle budding in the endoplasmic reticulum Mus musculus
3.4.24.B9 CGS 27023
-
 Mus musculus
3.4.24.B9 coban complete blockage of enzyme processing at 0.025 mg/ml Mus musculus
3.4.24.B9 marimastat
-
 Mus musculus
3.4.24.B9 PKVCGYLK inhibition less tan 20% at 0.1 mM Mus musculus
3.4.24.B9 PLKCGVSN inhibition less tan 20% at 0.1 mM Mus musculus
3.4.24.B9 PLRCGVSN inhibition less tan 20% at 0.1 mM Mus musculus
3.4.24.B9 QGGCADHS inhibition less tan 20% at 0.1 mM Mus musculus
3.4.24.B9 QGLCGSQH inhibition less tan 20% at 0.1 mM Mus musculus
3.4.24.B9 TNF-alpha protease inhibitor
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B9 membrane anchored on the cell surface Mus musculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B9 additional information metalloprotease Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.B9 12000
-
 x * 84000, processed enzyme, x * 12000, enzyme proform, SDS-PAGE Mus musculus
3.4.24.B9 84000
-
 x * 84000, processed enzyme, x * 12000, enzyme proform, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B9 additional information Mus musculus regulation of metalloprotease desintegrins via a cysteine-switch mechanism ?
-
 ?
3.4.24.B9 proteins + H2O Mus musculus
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B9 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.B9 glycoprotein N-glycosylation of endopeptidase H-sensitive precursor and mature enzyme Mus musculus
3.4.24.B9 proteolytic modification cleavage of pro-domain by furin-type pro-protein convertase before the protein is secreted to the cell surface Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B9 recombinant soluble enzyme from COS-7 cells Mus musculus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.B9 additional information
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B9 EVHHQKLVFFAE + H2O peptides derived from beta-amyloid precursor protein, comprises the predicted cleavage site Mus musculus EVHH + QKLVFFAE
-
 ?
3.4.24.B9 insulin B chain + H2O cleavage sites are Tyr16-Leu17 and Tyr26-Thr27 Mus musculus 3 peptides
-
 ?
3.4.24.B9 additional information regulation of metalloprotease desintegrins via a cysteine-switch mechanism Mus musculus ?
-
 ?
3.4.24.B9 proteins + H2O
-
 Mus musculus peptides
-
 ?
3.4.24.B9 SMAPGAVHLPQP + H2O peptides derived from P75 TNF-receptor, comprises the predicted cleavage site Mus musculus SMAPGAVH + LPQP
-
 ?
3.4.24.B9 SPLAQAVRSSSR + H2O peptides derived from TNF-alpha, comprises the predicted cleavage site Mus musculus SPLA + QAVRSSSR
-
 ?
3.4.24.B9 SPLAQAVRSSSR + H2O peptides derived from TNF-alpha, comprises the predicted cleavage site Mus musculus SPLAQAVRS + SSR
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.B9 ? x * 84000, processed enzyme, x * 12000, enzyme proform, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B9 M12.209 Merops-ID Mus musculus
3.4.24.B9 MDC9
-
 Mus musculus
3.4.24.B9 metallo protease desintegrin 9
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.B9 7.2
-
 assay at Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.B9 0.000014
-
 batimastat pH 7.5 Mus musculus
3.4.24.B9 0.000017
-
 TNF-alpha protease inhibitor pH 7.5 Mus musculus
3.4.24.B9 0.000054
-
 CGS 27023 pH 7.5 Mus musculus
3.4.24.B9 0.00027
-
 marimastat pH 7.5 Mus musculus
3.4.24.B9 0.004
-
 PKVCGYLK pH 7.5 Mus musculus
3.4.24.B9 0.01
-
 PLRCGVSN pH 7.5 Mus musculus
3.4.24.B9 0.037
-
 PLKCGVSN pH 7.5 Mus musculus
3.4.24.B9 0.15
-
 QGLCGSQH pH 7.5 Mus musculus
3.4.24.B9 0.15
-
 QGGCADHS pH 7.5 Mus musculus