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Literature summary extracted from

  • Ikeda, K.; Emoto, N.; Raharjo, S.B.; Nurhantari, Y.; Saiki, K.; Yokoyama, M.; Matsuo, M.
    Molecular identification and characterization of novel membrane-bound metalloprotease, the soluble secreted form of which hydrolyzes a variety of vasoactive peptides (1999), J. Biol. Chem., 274, 32469-32477.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B14 isozymes SEP and SEPDELTA, DNA determination and analysis, transient expression of both isoforms in CHO cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B14 1,10-phenanthroline inhibition of the soluble isozyme SEP Mus musculus
3.4.24.B14 additional information no inhibition by captopril Mus musculus
3.4.24.B14 phosphoramidon inhibition of the soluble isozyme SEP Mus musculus
3.4.24.B14 thiorphan inhibition of the soluble isozyme SEP Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B14 extracellular isozyme SEP also is secreted Mus musculus
-
-
3.4.24.B14 membrane isozymes SEP and SEPDELTA Mus musculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B14 Zn2+ zinc-metalloprotease Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.B14 110000
-
x * 110000, membrane-bound isozymes SEP and SEPDELTA, SDS-PAGE, x * 126000, soluble isozyme SEP, SDS-PAGE Mus musculus
3.4.24.B14 126000
-
x * 110000, membrane-bound isozymes SEP and SEPDELTA, SDS-PAGE, x * 126000, soluble isozyme SEP, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B14 protein + H2O Mus musculus
-
peptides
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B14 Mus musculus Q9JLI3 2 isozymes SEP and splicing variant SEPDELTA which lacks 69 base pair nucleotide segment following the transmembrane helix
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.B14 glycoprotein soluble isozyme SEP, and membrane-bound isozyme SEP and SEPDELTA Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.B14 adrenal gland isozymes SEP and SEPDELTA Mus musculus
-
3.4.24.B14 brain isozymes SEP and SEPDELTA Mus musculus
-
3.4.24.B14 heart isozymes SEP and SEPDELTA Mus musculus
-
3.4.24.B14 intestine isozymes SEP and SEPDELTA Mus musculus
-
3.4.24.B14 kidney isozymes SEP and SEPDELTA Mus musculus
-
3.4.24.B14 liver isozymes SEP and SEPDELTA Mus musculus
-
3.4.24.B14 lung isozymes SEP and SEPDELTA Mus musculus
-
3.4.24.B14 additional information in most cases isozyme SEPDELTA shows higher expression rates than isozyme SEP Mus musculus
-
3.4.24.B14 ovary isozyme SEP Mus musculus
-
3.4.24.B14 testis isozyme SEP, very low amount of isozyme SEPDELTA Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B14 angiotensin I + H2O
-
Mus musculus ?
-
?
3.4.24.B14 atrial natriuretic peptide + H2O
-
Mus musculus ?
-
?
3.4.24.B14 bradykinin + H2O
-
Mus musculus ?
-
?
3.4.24.B14 endothelin-1 + H2O
-
Mus musculus ?
-
?
3.4.24.B14 protein + H2O
-
Mus musculus peptides
-
?
3.4.24.B14 protein + H2O broad substrate specificity Mus musculus peptides
-
?
3.4.24.B14 Substance P + H2O
-
Mus musculus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.24.B14 ? x * 110000, membrane-bound isozymes SEP and SEPDELTA, SDS-PAGE, x * 126000, soluble isozyme SEP, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B14 M13.008 Merops-ID Mus musculus
3.4.24.B14 More may be identical with neprilysin EC 3.4.24.11 Mus musculus
3.4.24.B14 SEP
-
Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.B14 7.4
-
sharp pH-dependence Mus musculus