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Literature summary extracted from

  • Fushimi, N.; Ee, C.E.; Nakajima, T.; Ichishima, E.
    Aspzincin, a family of metalloendopeptidases with a new zinc-binding motif. Identification of new zinc-binding sites (His(128), His(132), and Asp(164)) and three catalytically crucial residues (Glu(129), Asp(143), and Tyr(106)) of deuterolysin from Aspergillus oryzae by site-directed mutagenesis (1999), J. Biol. Chem., 274, 24195-24201.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.39 recombinant enzyme expressed in Escherichia coli Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.39 D104N site-directed mutagenesis Aspergillus oryzae
3.4.24.39 D121N site-directed mutagenesis Aspergillus oryzae
3.4.24.39 D143E site-directed mutagenesis Aspergillus oryzae
3.4.24.39 D143N site-directed mutagenesis Aspergillus oryzae
3.4.24.39 D164N site-directed mutagenesis Aspergillus oryzae
3.4.24.39 D80N site-directed mutagenesis Aspergillus oryzae
3.4.24.39 E129D site-directed mutagenesis Aspergillus oryzae
3.4.24.39 E129Q site-directed mutagenesis Aspergillus oryzae
3.4.24.39 E142Q site-directed mutagenesis Aspergillus oryzae
3.4.24.39 E42Q site-directed mutagenesis Aspergillus oryzae
3.4.24.39 E65Q site-directed mutagenesis Aspergillus oryzae
3.4.24.39 E86Q site-directed mutagenesis Aspergillus oryzae
3.4.24.39 H118A site-directed mutagenesis Aspergillus oryzae
3.4.24.39 H128R site-directed mutagenesis Aspergillus oryzae
3.4.24.39 H132R site-directed mutagenesis Aspergillus oryzae
3.4.24.39 R58Q site-directed mutagenesis Aspergillus oryzae
3.4.24.39 Y106F site-directed mutagenesis Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.39 Zn2+ contains 1 g-atom of zinc/mol of enzyme Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.39 19020
-
 amino acid sequence Aspergillus oryzae
3.4.24.39 19800
-
 recombinant enzyme, gel filtration Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.39 Aspergillus oryzae
-
 mold
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.39 recombinant prodeuterolysin Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.39 salmon protamine sulfate + H2O
-
 Aspergillus oryzae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.39 aspzincin
-
 Aspergillus oryzae
3.4.24.39 Neutral proteinase II fornerly designated as Aspergillus oryzae