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Literature summary extracted from

  • Vazquez, F.; Hastings, G.; Ortega, M.A.; Lane, T.F.; Oikemus, S.; Lombardo, M.; Iruela-Arispe, M.L.
    METH-1, a human ortholog of ADAMTS-1, and METH-2 are members of a new family of proteins with angio-inhibitory activity (1999), J. Biol. Chem., 274, 23349-23357.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B11 DNA and amino acid sequence determination and analysis, in itro translation, expression as His-tagged protein in Escherichia coli strain BL21 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B11 extracellular secretion Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.B11 67000
-
 x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE Homo sapiens
3.4.24.B11 85000
-
 x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE Homo sapiens
3.4.24.B11 110000
-
 x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B11 additional information Homo sapiens enzyme inhibits endothelial cell proliferation, but not fibroblast or smooth muscle growth ?
-
 ?
3.4.24.B11 proteins + H2O Homo sapiens
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B11 Homo sapiens Q9UHI8 single copy gene
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.B11 proteolytic modification cleavage at the consensus subtilisin site RKKR resulting in 2 different processed forms of MW 85 kDa and 67 kDa Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B11 recombinant His-tagged enzyme from Escherichia coli strain BL21 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.B11 adrenal gland
-
 Homo sapiens
-
3.4.24.B11 heart
-
 Homo sapiens
-
3.4.24.B11 kidney embryonic Homo sapiens
-
3.4.24.B11 additional information no activity in brain, liver, pancreas Homo sapiens
-
3.4.24.B11 placenta
-
 Homo sapiens
-
3.4.24.B11 skeletal muscle
-
 Homo sapiens
-
3.4.24.B11 stomach
-
 Homo sapiens
-
3.4.24.B11 thyroid gland
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B11 additional information enzyme inhibits fibroblast growth factor 2-induced vascularization in the cornea pocket assay and inhibits vascular endothelial growth factor-induced angiogenesis in the chorioallantoic membrane assay Homo sapiens ?
-
 ?
3.4.24.B11 additional information enzyme inhibits endothelial cell proliferation, but not fibroblast or smooth muscle growth Homo sapiens ?
-
 ?
3.4.24.B11 proteins + H2O
-
 Homo sapiens peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.B11 ? x * 67000, recombinant processed form 2, SDS-PAGE, x * 85000, recombinant processed form 1, SDS-PAGE, x * 110000, recombinant unprocessed enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B11 M12.222 Merops-ID Homo sapiens
3.4.24.B11 METH-1
-
 Homo sapiens