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Literature summary extracted from

  • Sahay, N.; Guy, H.I.; Liu, X.; Evans, D.R.
    Regulation of an Escherichia coli/mammalian chimeric carbamoyl-phosphate synthetase (1998), J. Biol. Chem., 273, 31195-31202.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 phosphoribosyl 5'-diphosphate activation of Escherichia coli and chimeric enzyme, the presence of UTP reduces the PRPP binding Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.5
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.5 additional information a chimeric deletion mutant A1A2B3 lacks 59 of 163 residues from the carboxyl end of the Escherichia coli /mammalian chimera, this mutant is somewhat more active than the full-length chimeric protein but has reduced sensitivity to allosteric effectors Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 UMP not chimeric protein Escherichia coli
6.3.5.5 UTP inhibits activity of Escherichia coli and chimeric enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.5 0.43
-
 ATP
-
 Escherichia coli
6.3.5.5 0.46
-
 ATP chimeric enzyme, in comparison to effect of phosphorylation and presence of effectors Escherichia coli
6.3.5.5 0.53
-
 ATP deletion mutant, in comparison to the presence of effectors Escherichia coli
6.3.5.5 2.14
-
 ATP
-
 Mammalia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.5 58000
-
 2 * 58000, SDS-PAGE, chimeric protein Escherichia coli
6.3.5.5 120000
-
 chimeric protein Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Escherichia coli
-
-
-
6.3.5.5 Mammalia
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.3.5.5 side-chain modification construction of a chimeric enzyme consisting of Escherichia coli subdomains A1 and A2 fused to the mammalian enzyme subdomain B3 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Mammalia 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
 Mammalia 2 ADP + phosphate + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
 Escherichia coli 2 ADP + phosphate + carbamoyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.5 dimer 2 * 58000, SDS-PAGE, chimeric protein Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.5 0.78
-
 ATP
-
 Mammalia
6.3.5.5 3.35
-
 ATP chimeric enzyme, in comparison to effect of phosphorylation and presence of effectors Escherichia coli
6.3.5.5 5.3
-
 ATP deletion mutant, in comparison to the presence of effectors Escherichia coli
6.3.5.5 6.08
-
 ATP
-
 Mammalia
6.3.5.5 8
-
 ATP
-
 Escherichia coli