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Literature summary extracted from

  • Potempa, J.; Mikolajczyk-Pawlinska, J.; Brassell, D.; Nelson, D.; Thogersen, I.B.; Enghild, J.J.; Travis, J.
    Comparative properties of two cysteine proteinases (gingipains R), the products of two related but individual genes of Porphyromonas gingivalis (1998), J. Biol. Chem., 273, 21648-21657.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.37 2-mercaptoethanol weak activation, a concentration above 100 mM is required Porphyromonas gingivalis
3.4.22.37 cysteamine activates, best above 5 mM Porphyromonas gingivalis
3.4.22.37 cysteine 10 mM, most effective reducing agent for activation of the enzyme, denaturation occurs at higher concentration above 5 mM Porphyromonas gingivalis
3.4.22.37 dithiothreitol weak activation, a concentration above 100 mM is required Porphyromonas gingivalis
3.4.22.37 glutathione activates, best above 5 mM Porphyromonas gingivalis
3.4.22.37 glycyl-glycine stimulates the amidolytic activity and limited proteolysis, but destabilizes the enzyme Porphyromonas gingivalis
3.4.22.37 glycyl-glycine stimulates the amidolytic activity and limited proteolysis, but destabilizes the isozymes Porphyromonas gingivalis

General Stability

EC Number General Stability Organism
3.4.22.37 Ca2+ stabilizes the enzyme, while glycyl-glycine destabilizes the enzyme Porphyromonas gingivalis
3.4.22.37 Ca2+ stabilizes the isozymes, while glycyl-glycine destabilizes the isozymes Porphyromonas gingivalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.37 additional information
-
 additional information
-
 Porphyromonas gingivalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.37 extracellular secretion Porphyromonas gingivalis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.37 Ca2+ stabilizes the activity Porphyromonas gingivalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.37 48120 48150 mature, C-terminally truncated isozymes, amino acid sequence calculation and mass spectroscopy Porphyromonas gingivalis
3.4.22.37 48120 48150 mature, C-terminally truncated isozymes, amino acid sequence calculation, gel filtration and mass spectroscopy Porphyromonas gingivalis
3.4.22.37 95000
-
 a complex of catalytic and hemagglutinin/adhesin domains, gel filtration Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.37 protein + H2O Porphyromonas gingivalis
-
 peptides
-
 ?
3.4.22.37 protein + H2O Porphyromonas gingivalis HG66
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.37 Porphyromonas gingivalis
-
 gene rgp-1, enzyme gingipain R1
-
3.4.22.37 Porphyromonas gingivalis B2RKU0 strain ATCC33277, gene rgpB gene, enzyme gingipain R2, 4 isoforms I-IV that are indistinguishable with regard to stability, pH-optima, kinetic characteristics and proteolytic activity
-
3.4.22.37 Porphyromonas gingivalis Q51844 gene rgpB/rgp-2 gene, enzyme gingipain R2, 4 isoforms I-IV that are indistinguishable with regard to stability, pH-optima, kinetic characteristics and proteolytic activity
-
3.4.22.37 Porphyromonas gingivalis ATCC 33277 B2RKU0 strain ATCC33277, gene rgpB gene, enzyme gingipain R2, 4 isoforms I-IV that are indistinguishable with regard to stability, pH-optima, kinetic characteristics and proteolytic activity
-
3.4.22.37 Porphyromonas gingivalis HG66
-
 gene rgp-1, enzyme gingipain R1
-
3.4.22.37 Porphyromonas gingivalis HG66 Q51844 gene rgpB/rgp-2 gene, enzyme gingipain R2, 4 isoforms I-IV that are indistinguishable with regard to stability, pH-optima, kinetic characteristics and proteolytic activity
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.37 proteolytic modification the mature enzyme is C-terminally truncated to 433 amino acid residues Porphyromonas gingivalis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.37 from cell culture medium Porphyromonas gingivalis
3.4.22.37 isozymes I-IV from cell culture medium, 4.5-5fold Porphyromonas gingivalis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.22.37 hydrolysis of proteins and small molecule substrates, with a preference for Arg in P1 proteolysis of selected proteins Porphyromonas gingivalis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.37 40.8
-
 purified isozyme IV Porphyromonas gingivalis
3.4.22.37 41.3
-
 purified isozyme I Porphyromonas gingivalis
3.4.22.37 42.9
-
 purified isozyme III Porphyromonas gingivalis
3.4.22.37 45.5
-
 purified isozyme II Porphyromonas gingivalis

Storage Stability

EC Number Storage Stability Organism
3.4.22.37 -20°C, buffer with neutral pH, 1-5 mM CaCl2, indefinitely stable Porphyromonas gingivalis
3.4.22.37 0°C, on ice, buffer with neutral pH, 1-5 mM CaCl2, stable for months Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.37 alpha1-Antichymotrypsin + H2O inactivation of the substrate Porphyromonas gingivalis ?
-
 ?
3.4.22.37 alpha1-Antichymotrypsin + H2O inactivation of the substrate Porphyromonas gingivalis HG66 ?
-
 ?
3.4.22.37 azocasein + H2O synthetic chromogenic substrate Porphyromonas gingivalis ?
-
 ?
3.4.22.37 azocasein + H2O synthetic chromogenic substrate Porphyromonas gingivalis HG66 ?
-
 ?
3.4.22.37 benzoyl-L-arginine-4-nitroanilide + H2O synthetic fluorogenic substrate Porphyromonas gingivalis benzoyl-L-arginine + 4-nitroaniline
-
 ?
3.4.22.37 denatured alpha1-proteinase inhibitor + H2O
-
 Porphyromonas gingivalis ?
-
 ?
3.4.22.37 denatured type I collagen + H2O
-
 Porphyromonas gingivalis ?
-
 ?
3.4.22.37 denatured type I collagen + H2O
-
 Porphyromonas gingivalis HG66 ?
-
 ?
3.4.22.37 Leu-Tyr-Arg-4-nitroanilide + H2O synthetic fluorogenic substrate Porphyromonas gingivalis Leu-Tyr-Arg + 4-nitroaniline
-
 ?
3.4.22.37 additional information substrate specificity, no inactivation of native alpha1-proteinase inhibitor and native type I collagen Porphyromonas gingivalis ?
-
 ?
3.4.22.37 additional information substrate specificity, no inactivation of native alpha1-proteinase inhibitor and native type I collagen Porphyromonas gingivalis HG66 ?
-
 ?
3.4.22.37 protein + H2O
-
 Porphyromonas gingivalis peptides
-
 ?
3.4.22.37 protein + H2O
-
 Porphyromonas gingivalis HG66 peptides
-
 ?
3.4.22.37 tosyl-GPR-4-nitroanilide + H2O other substrates with a Ps proline are very poor substrates, synthetic fluorogenic substrate Porphyromonas gingivalis tosyl-GPR + 4-nitroaniline
-
 ?
3.4.22.37 Z-Arg-Arg-4-nitroanilide + H2O synthetic fluorogenic substrate Porphyromonas gingivalis Z-Arg-Arg + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.37 monomer 1 * 48019-48369, gingipain R2 isozymes I-IV and laser mass spectroscopy Porphyromonas gingivalis
3.4.22.37 monomer 1 * 48019-48369, gingipain R2 isozymes I-IV, laser mass spectroscopy Porphyromonas gingivalis
3.4.22.37 More the enzyme complex consists of 4 major polypeptides of 49.5 kDa for the catalytic domain, 43 kDa, 23.3 kDa, and 15.9 kDa Porphyromonas gingivalis

Synonyms

EC Number Synonyms Comment Organism
3.4.22.37 arginine-specific cysteine proteinase
-
 Porphyromonas gingivalis
3.4.22.37 gingipain R2 two different enzymes gingipain R exist in Porphyromonas gingivalis, that are encoded by related but individual genes Porphyromonas gingivalis
3.4.22.37 RGP-2
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.37 37
-
 assay at Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.37 7.5
-
 protein substrates Porphyromonas gingivalis
3.4.22.37 8
-
 4-nitroanilide substrates Porphyromonas gingivalis
3.4.22.37 9.5
-
 protein substrates Porphyromonas gingivalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.37 5 9.5 50% activity at pH 5.0, maximal activity at pH 9.5 Porphyromonas gingivalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.37 Porphyromonas gingivalis isoelectric focusing
-
 3.74
3.4.22.37 Porphyromonas gingivalis isozyme IV, isoelectric focusing
-
 4.96
3.4.22.37 Porphyromonas gingivalis isozyme III, isoelectric focusing
-
 5.03
3.4.22.37 Porphyromonas gingivalis isozyme II, isoelectric focusing
-
 5.09
3.4.22.37 Porphyromonas gingivalis isozyme I, isoelectric focusing
-
 5.21