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Literature summary extracted from

  • Wang, W.; Gorrell, A.; Hou, Z.; Honzatko, R.B.; Fromm, H.J.
    Ambiguities in mapping the active site of a conformationally dynamic enzyme by directed mutation. Role of dynamics in structure-function correlations in Escherichia coli adenylosuccinate synthetase (1998), J. Biol. Chem., 273, 16000-16004.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.4 purA gene Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.4
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.4 D21A directed mutagenesis Escherichia coli
6.3.4.4 N38A directed mutagenesis Escherichia coli
6.3.4.4 N38D directed mutagenesis Escherichia coli
6.3.4.4 N38E directed mutagenesis Escherichia coli
6.3.4.4 R419L directed mutagenesis Escherichia coli
6.3.4.4 T42A directed mutagenesis Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.4 0.011
-
 GTP pH 5.6, 25°C, wild-type Escherichia coli
6.3.4.4 0.026
-
 GTP pH 7.7, 25°C, wild-type Escherichia coli
6.3.4.4 0.028
-
 IMP pH 7.7, 25°C, wild-type and mutant N38E Escherichia coli
6.3.4.4 0.041
-
 GTP pH 7.7, 25°C, mutant N38A Escherichia coli
6.3.4.4 0.048
-
 IMP pH 7.7, 25°C, mutant D21A Escherichia coli
6.3.4.4 0.049
-
 IMP pH 7.7, 25°C, mutant N38A Escherichia coli
6.3.4.4 0.052
-
 GTP pH 7.7, 25°C, mutant D21A Escherichia coli
6.3.4.4 0.054
-
 GTP pH 7.7, 25°C, mutant N38E Escherichia coli
6.3.4.4 0.07
-
 IMP pH 5.6, 25°C, wild-type Escherichia coli
6.3.4.4 0.116
-
 GTP pH 5.6, 25°C, mutant N38D Escherichia coli
6.3.4.4 0.123
-
 IMP pH 7.7, 25°C, mutant T42A Escherichia coli
6.3.4.4 0.13
-
 GTP pH 7.7, 25°C, mutant H41N Escherichia coli
6.3.4.4 0.17
-
 aspartate pH 7.7, 25°C, mutant D21A Escherichia coli
6.3.4.4 0.2
-
 IMP pH 7.7, 25°C, mutant R419L Escherichia coli
6.3.4.4 0.23
-
 aspartate pH 7.7, 25°C, wild-type Escherichia coli
6.3.4.4 0.24
-
 aspartate pH 7.7, 25°C, mutant N38E Escherichia coli
6.3.4.4 0.25
-
 GTP pH 7.7, 25°C, mutant R419L Escherichia coli
6.3.4.4 0.27
-
 GTP pH 7.7, 25°C, mutant N38D Escherichia coli
6.3.4.4 0.28
-
 GTP pH 7.7, 25°C, mutant T42A Escherichia coli
6.3.4.4 0.3
-
 aspartate pH 7.7, 25°C, mutant N38A Escherichia coli
6.3.4.4 0.34
-
 aspartate pH 7.7, 25°C, mutant R419L Escherichia coli
6.3.4.4 0.4
-
 IMP pH 7.7, 25°C, mutant H41N Escherichia coli
6.3.4.4 0.5
-
 aspartate pH 5.6, 25°C, mutant N38D Escherichia coli
6.3.4.4 0.9
-
 aspartate pH 5.6, 25°C, wild-type Escherichia coli
6.3.4.4 1.4
-
 aspartate pH 7.7, 25°C, mutant T42A Escherichia coli
6.3.4.4 1.6
-
 IMP pH 7.7, 25°C, mutant N38D Escherichia coli
6.3.4.4 2
-
 aspartate pH 7.7, 25°C, mutant H41N Escherichia coli
6.3.4.4 2.6
-
 aspartate pH 7.7, 25°C, mutant N38D Escherichia coli
6.3.4.4 3.8
-
 IMP pH 5.6, 25°C, mutant N38D Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.4 48000
-
 SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.4 GTP + IMP + L-aspartate Escherichia coli catalyzing the first committed step in the biosynthesis of AMP GDP + phosphate + adenylosuccinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.4 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.4
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.4 GTP + IMP + L-aspartate
-
 Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 GTP + IMP + L-aspartate catalyzing the first committed step in the biosynthesis of AMP Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.4 adenosylsuccinate synthetase
-
 Escherichia coli
6.3.4.4 AMPSase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.4 0.0048
-
 GTP pH 7.7, 25°C, mutant N38A Escherichia coli
6.3.4.4 0.0095
-
 GTP pH 7.7, 25°C, mutant H41N Escherichia coli
6.3.4.4 0.0134
-
 GTP pH 7.7, 25°C, mutant N38D Escherichia coli
6.3.4.4 0.034
-
 GTP pH 7.7, 25°C, mutant N38E Escherichia coli
6.3.4.4 0.049
-
 GTP pH 7.7, 25°C, mutant D21A Escherichia coli
6.3.4.4 0.096
-
 GTP pH 5.6, 25°C, wild-type Escherichia coli
6.3.4.4 0.1
-
 GTP pH 7.7, 25°C, mutant R419L Escherichia coli
6.3.4.4 0.171
-
 GTP pH 5.6, 25°C, mutant N38D Escherichia coli
6.3.4.4 0.89
-
 GTP pH 7.7, 25°C, mutant T42A Escherichia coli
6.3.4.4 1
-
 GTP pH 7.7, 25°C, wild-type Escherichia coli
6.3.4.4 1.59
-
 GTP pH 7.8, 25°C, wild-type Escherichia coli
6.3.4.4 2.94
-
 GTP pH 7.8, 25°C, wild-type Escherichia coli
6.3.4.4 6.08
-
 GTP pH 7.7, 25°C, mutant T42A Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.4 7.8
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.4 5.2 8
-
 Escherichia coli