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Literature summary extracted from

  • Kessler, E.; Safrin, M.; Abrams, W.R.; Rosenbloom, J.; Ohman, D.E.
    Inhibitors and specificity of Pseudomonas aeruginosa LasA (1997), J. Biol. Chem., 272, 9884-9889.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B16 1,10-phenanthroline inhibition of the staphylolytic activity Pseudomonas aeruginosa
3.4.24.B16 dithiothreitol inhibition of the staphylolytic activity Pseudomonas aeruginosa
3.4.24.B16 EDTA inhibition only above 20 mM Pseudomonas aeruginosa
3.4.24.B16 EGTA inhibition only above 20 mM Pseudomonas aeruginosa
3.4.24.B16 additional information no inhibition by 1,7-penanthroline and 4,7-phenanthroline, activity with beta-caseinis blocked by inhibitors that do not block the staphylolytic activity, no inhibition by several serine or cysteine proteinase inhibitors including diisopropyl fluorophosphate, phenylmethylsulfonylfluoride, leupeptin, and N-ethylmaleimide Pseudomonas aeruginosa
3.4.24.B16 Nalpha-p-tosyl-L-lysine chloromethyl ketone inhibition of the staphylolytic activity Pseudomonas aeruginosa
3.4.24.B16 phosphoramidon inhibition of the staphylolytic activity Pseudomonas aeruginosa
3.4.24.B16 Tetraethylenepentamine inhibition of the staphylolytic activity Pseudomonas aeruginosa
3.4.24.B16 Zn2+ in excess, inhibition of staphylolytic activity Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B16 extracellular
-
 Pseudomonas aeruginosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B16 Zn2+ zinc-metalloprotease Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B16 Elastin + H2O Pseudomonas aeruginosa enzyme activates elastase, and the elastolytic activity of other proteases by cleaving elastin ?
-
 ?
3.4.24.B16 additional information Pseudomonas aeruginosa enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells by cleaving the peptidoglycan pentaglycine interpeptides ?
-
 ?
3.4.24.B16 protein + H2O Pseudomonas aeruginosa
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B16 Pseudomonas aeruginosa
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.B16 proteolytic degradation of proteins specific for Gly-Ala peptide bonds within Gly-Gly-Ala sequences in elastin, substrates that contain no Gly-Gly peptide bond, such as beta-casein, appear to be resistant to the enzyme Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B16 Elastin + H2O soluble recombinant and insoluble elastin Pseudomonas aeruginosa ?
-
 ?
3.4.24.B16 Elastin + H2O enzyme activates elastase, and the elastolytic activity of other proteases by cleaving elastin Pseudomonas aeruginosa ?
-
 ?
3.4.24.B16 additional information enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells by cleaving the peptidoglycan pentaglycine interpeptides Pseudomonas aeruginosa ?
-
 ?
3.4.24.B16 protein + H2O
-
 Pseudomonas aeruginosa peptides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B16 M23.002 Merops-ID Pseudomonas aeruginosa
3.4.24.B16 staphylolysin
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.B16 37
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.B16 8.5
-
 assay at Pseudomonas aeruginosa