Literature summary extracted from

Pineiro-Sanchez, M.L.; Goldstein, L.A.; Dodt, J.; Howard, L.; Yeh, Y.; Tran, H.; Argraves, W.S.; Chen, W.T.
Identification of the 170-kDa melanoma membrane-bound gelatinase (seprase) as a serine integral membrane protease (1997), J. Biol. Chem., 272, 7595-7601.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.4.21.B28	trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane	activation	Homo sapiens

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.21.B28	-	Homo sapiens

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.21.B28	4-(2-aminoethyl)-benzenesulfonyl fluoride hydrochloride	AEBSF, inhibition at 5 mM	Homo sapiens
3.4.21.B28	4-amidino phenylsulfonyl fluoride	APSF, inhibition at 0.05 mM	Homo sapiens
3.4.21.B28	diisopropylfluorophosphate	inhibition at 0.005 mM	Homo sapiens
3.4.21.B28	phenylmethylsulfonyl fluoride	-	Homo sapiens

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.4.21.B28	membrane	-	Homo sapiens	16020	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.4.21.B28	97000	-	inactive subunit, SDS-PAGE	Homo sapiens
3.4.21.B28	170000	-	active dimer	Homo sapiens
3.4.21.B28	400000	-	above 400000 Da, complex in nonionic detergents, gel filtration	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.21.B28	Homo sapiens	Q12884	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
3.4.21.B28	glycoprotein	N-glycosylation	Homo sapiens

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.4.21.B28	LOX cell	human melanoma cell	Homo sapiens	-

Subunits

EC Number	Subunits	Comment	Organism
3.4.21.B28	dimer	SDS-PAGE	Homo sapiens

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.21.B28	gelatinase	-	Homo sapiens
3.4.21.B28	seprase	-	Homo sapiens

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Release 2023.1 (January 2023)