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Literature summary extracted from

  • Renatus, M.; Bode, W.; Huber, R.; Sturzebecher, J.; Prasa, D.; Fischer, S.; Kohnert, U.; Stubbs, M.T.
    Structural mapping of the active site specificity determinants of human tissue-type plasminogen activator. Implications for the design of low molecular weight substrates and inhibitors (1997), J. Biol. Chem., 272, 21713-21719.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.68 medicine t-PA has been widely and successfully used as a therapeutic fibrinolytic agent to treat acute myocardial infarction and pulmonary embolism Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.68
-
 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.68 bis-benzamidine-tPA complex, spacegroup C2, cell constants a : 151.83 A, b : 60.50 A, c =:62.61 A Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.68 0.1
-
 methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.3
-
 benzyloxycarbonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.34
-
 methyloxycarbonyl-D-cyclohexylalanyl-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.39
-
 methyloxycarbonyl-D-hexahydrotyrosyl-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.42
-
 methyloxycarbonyl-D-cyclohexylglycyl-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.52
-
 benzyloxycarbonyl-D-Arg-Gly-Arg-4-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.62
-
 methyloxycarbonyl-D-Leu-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.7
-
 D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.72
-
 benzyloxycarbonyl-Asp-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.8
-
 methyloxycarbonyl-D-Val-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.83
-
 D-hexahydrotyrosyl-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.86
-
 Asp-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.9
-
 benzyloxycarbonyl-Glu-(O-tert-butyl)Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 0.99
-
 methyloxycarbonyl-D-Nle-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 1.2
-
 benzyloxycarbonyl-D-Glu(O-tetr-butyl)-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 1.36
-
 benzyloxycarbonyl-Val-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 1.53
-
 D-cyclohexylglycyl-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 1.7
-
 D-Asp(O-tert-butyl)-Gly-Arg-4-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 1.8
-
 benzyloxycarbonyl-D-Asp(O-tert-butyl)-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 2
-
 Phe-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 2.1
-
 D-Lys-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 2.2
-
 benzyloxycarbonyl-Glu-Gly-Arg-4-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 2.3
-
 benzyloxycarbonyl-Asp(O-tert-butyl)-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 2.6
-
 benzyloxycarbonyl-D-Glu-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 2.9
-
 D-Glu-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 3.15
-
 pGlu-Gly-Arg-4-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 3.2
-
 Glu-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 3.5
-
 tert-butoxycarbonyl-Gly-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens
3.4.21.68 5.32
-
 cyclohexylglycyl-Gly-Arg-p-nitroanilide pH 8.0, 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.68 plasminogen + H2O Homo sapiens
-
 plasmin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.68 Homo sapiens
-
 human
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.68 Asp-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens Asp-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 benzyloxycarbonyl-Asp(O-tert-butyl)-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-Asp(O-tert-butyl)-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 benzyloxycarbonyl-Asp-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-Asp-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 benzyloxycarbonyl-D-Arg-Gly-Arg-4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-D-Arg-Gly-Arg + 4-nitroaniline
-
 ?
3.4.21.68 benzyloxycarbonyl-D-Asp(O-tert-butyl)-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-D-Asp(O-tert-butyl)-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 benzyloxycarbonyl-D-Glu(O-tert-butyl)-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-D-Glu-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 benzyloxycarbonyl-D-Glu-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-D-Glu-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 benzyloxycarbonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-D-Phe-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 benzyloxycarbonyl-Glu(O-tert-butyl)-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-Glu(O-tert-butyl)-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 benzyloxycarbonyl-Glu-Gly-Arg-4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-Glu-Gly-Arg + 4-nitroaniline
-
 ?
3.4.21.68 benzyloxycarbonyl-pyroglutamic acid-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-pyroglutamic acid-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 benzyloxycarbonyl-Val-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-Val-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 cyclohexylglycyl-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens cyclohexylglycyl-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 D-Asp(O-tert-butyl)-Gly-Arg-4-nitroanilide + H2O
-
 Homo sapiens D-Asp(O-tert-butyl)-Gly-Arg + 4-nitroaniline
-
 ?
3.4.21.68 D-Asp-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens D-Asp-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 D-cyclohexylglycyl-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens D-cyclohexylglycyl-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 D-Glu-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens D-Glu-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 D-hexahydrotyrosyl-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens D-hexahydrotyrosyl-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 D-Lys-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens D-Lys-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 D-Phe-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens D-Phe-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 Glu-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens Glu-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 methyloxycarbonyl-D-cyclohexylalanyl-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens methyloxycarbonyl-D-cyclohexylglycyl-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 methyloxycarbonyl-D-cyclohexylglycyl-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens methyloxycarbonyl-D-cyclohexylglycyl-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 methyloxycarbonyl-D-hexahydrotyrosyl-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens methyloxycarbonyl-D-hexahydrotyrosyl-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 methyloxycarbonyl-D-Leu-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens methyloxycarbonyl-D-Leu-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 methyloxycarbonyl-D-Nle-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens methyloxycarbonyl-D-Nle-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 methyloxycarbonyl-D-Val-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens methyloxycarbonyl-D-Nle-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens methylsulfonyl-D-Phe-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 pGlu-Gly-Arg-4-nitroanilide + H2O
-
 Homo sapiens pGlu-Gly-Arg + 4-nitroaniline
-
 ?
3.4.21.68 Phe-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens Phe-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.68 plasminogen + H2O
-
 Homo sapiens plasmin + ?
-
 ?
3.4.21.68 tert-butyloxycarbonyl-Gly-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens tert-butyloxycarbonyl-Gly-Gly-Arg + p-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.68 tissue-type plasmiongen activator
-
 Homo sapiens