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Literature summary extracted from

  • Ke, S.H.; Tachias, K.; Lamba, D.; Bode, W.; Madison, E.L.
    Identification of a hydrophobic exosite on tissue type plasminogen activator that modulates specificity for plasminogen (1997), J. Biol. Chem., 272, 1811-1816.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.68 Fibrin activity of wild-type t-PA is stimulated by Homo sapiens

Application

EC Number Application Comment Organism
3.4.21.68 medicine t-PA has been widely and successfully used as a therapeutic fibrinolytic agent to treat acute myocardial infarction and pulmonary embolism Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.68 hybridoma cell lines expressing Mabs expression of wild-type and mutant enzyme cDNA by transient transfection of COS cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.68 F423A two chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 F423A/R275E single-chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 F423E two chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 F423E/R275E single-chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 L420A two chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 L420A/R275E single-chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 L420E two chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 L420E/R275E single-chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 P422A two chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 P422A/R275E single-chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 P422E/R275E single-chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 P422G/R275E single-chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 R275E single-chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 R275E/P422E site-directed mutagenesis Homo sapiens
3.4.21.68 R275E/P422G site-directed mutagenesis Homo sapiens
3.4.21.68 S421E two chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 S421E/R275E single-chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 S421G two chain form, site-directed mutagenesis Homo sapiens
3.4.21.68 S421G/R275E single-chain form, site-directed mutagenesis Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.68 0.00001
-
 Lys-plasminogen pH 7.2, 37°C, mutant R275E and P422A/R275E, single-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000011
-
 Lys-plasminogen pH 7.2, 37°C, wild-type, two-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000013
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422A, two-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000014
-
 Lys-plasminogen pH 7.2, 37°C, two-chain form of mutant F423A, single-chain form of mutant L420A/R275E, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000015
-
 Lys-plasminogen pH 7.2, 37°C, mutant F423E/R275E, single-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000016
-
 Lys-plasminogen pH 7.2, 37°C, mutant S421G, two-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000018
-
 Lys-plasminogen pH 7.2, 37°C, two-chain form of mutant S421E, single-chain form of mutant L420E/R275E, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000019
-
 Lys-plasminogen pH 7.2, 37°C, mutant L420A, two-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000021
-
 Lys-plasminogen pH 7.2, 37°C, mutant L420E, two-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000022
-
 Lys-plasminogen pH 7.2, 37°C, mutant F423A/R275E, single-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000023
-
 Lys-plasminogen pH 7.2, 37°C, two-chain form of mutant F423E, single-chain form of mutant S421E/R275E, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000026
-
 Lys-plasminogen pH 7.2, 37°C, two-chain form of mutant P422G, single-chain form of mutant S421G/R275E, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000027
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422G/R275E, single-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000028
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422E/R275E, single-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.000029
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422E, two-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.0006
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422G, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.0007
-
 Lys-plasminogen pH 7.2, 37°C, mutant L420E and P422E, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.001
-
 Lys-plasminogen pH 7.2, 37°C, mutant L420A, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.003
-
 Lys-plasminogen pH 7.2, 37°C, mutant S421E, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.004
-
 Lys-plasminogen pH 7.2, 37°C, two-chain form of mutant S421G, F423A and P422A, single chain form of mutant S421G/R275E and P422G/R275E, absence of soluble fibrin Homo sapiens
3.4.21.68 0.005
-
 Lys-plasminogen pH 7.2, 37°C, mutant L420E/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.005
-
 Lys-plasminogen pH 7.2, 37°C, two-chain form of wild-type and mutant F423E, single-chain form of mutant P422E/R275E, absence of soluble fibrin Homo sapiens
3.4.21.68 0.007
-
 Lys-plasminogen pH 7.2, 37°C, mutant S421E/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.008
-
 Lys-plasminogen pH 7.2, 37°C, mutant F423E/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.009
-
 Lys-plasminogen pH 7.2, 37°C, mutant L420A/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.01
-
 Lys-plasminogen pH 7.2, 37°C, mutant F423A/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.013
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422A/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.017
-
 Lys-plasminogen pH 7.2, 37°C, mutant R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.4
-
 spectrozyme tPA pH 7.2, 37°C, wild-type t-PA, mutant L420A, L420E, S421G, S421E, P422A, P422E, two-chain form Homo sapiens
3.4.21.68 0.5
-
 spectrozyme tPA pH 7.2, 37°C, mutant F423A, F422E, two-chain form Homo sapiens
3.4.21.68 0.6
-
 spectrozyme tPA pH 7.2, 37°C, P422G, two-chain form Homo sapiens
3.4.21.68 0.9
-
 spectrozyme tPA pH 7.2, 37°C, mutant L420E/R275E, F423E/R275E, single chain form Homo sapiens
3.4.21.68 1
-
 spectrozyme tPA pH 7.2, 37°C, mutant R275E, F423A/R275E, single-chain form Homo sapiens
3.4.21.68 1.1
-
 spectrozyme tPA pH 7.2, 37°C, mutant P422E/R275E, single chain form Homo sapiens
3.4.21.68 1.2
-
 spectrozyme tPA pH 7.2, 37°C, mutant P422A/R275E, single chain form Homo sapiens
3.4.21.68 1.3
-
 spectrozyme tPA pH 7.2, 37°C, mutant L420A/R275E, single chain form Homo sapiens
3.4.21.68 1.7
-
 spectrozyme tPA pH 7.2, 37°C, mutant P422G/R275E, single chain form Homo sapiens
3.4.21.68 2.3
-
 spectrozyme tPA pH 7.2, 37°C, mutant S421E/R275E, single chain form Homo sapiens
3.4.21.68 4.6
-
 spectrozyme tPA pH 7.2, 37°C, mutant L420A/R275E, single chain form Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.68 plasminogen + H2O Homo sapiens
-
 plasmin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.68 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.68 wild type and recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.68 Lys-plasminogen + H2O
-
 Homo sapiens plasmin + ?
-
 ?
3.4.21.68 plasminogen + H2O
-
 Homo sapiens plasmin + ?
-
 ?
3.4.21.68 spectrozyme tPA + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.68 tissue type plasminogen activator
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.68 0.0005
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422E/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.0008
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422G/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.001
-
 Lys-plasminogen pH 7.2, 37°C, mutant S421G/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.0015
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422E, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.002
-
 Lys-plasminogen pH 7.2, 37°C, mutant L420E/R275E and S421E/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.0028
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422G, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.003
-
 Lys-plasminogen pH 7.2, 37°C, mutant F423E/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.004
-
 Lys-plasminogen pH 7.2, 37°C, mutant F423A/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.005
-
 Lys-plasminogen pH 7.2, 37°C, mutant L420E, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.007
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422A/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.008
-
 Lys-plasminogen pH 7.2, 37°C, mutant L420A/R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.014
-
 Lys-plasminogen pH 7.2, 37°C, mutant L420A, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.016
-
 Lys-plasminogen pH 7.2, 37°C, mutant S421E, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.017
-
 Lys-plasminogen pH 7.2, 37°C, mutant F423E, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.018
-
 Lys-plasminogen pH 7.2, 37°C, mutant S421G, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.02
-
 Lys-plasminogen pH 7.2, 37°C, mutant F423A, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.024
-
 Lys-plasminogen pH 7.2, 37°C, mutant R275E, single-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.028
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422A, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.068
-
 Lys-plasminogen pH 7.2, 37°C, wild-type, two-chain form, absence of soluble fibrin Homo sapiens
3.4.21.68 0.1
-
 Lys-plasminogen pH 7.2, 37°C, wild-type, two-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.11
-
 Lys-plasminogen pH 7.2, 37°C, mutant S421G and P422A, two-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.12
-
 Lys-plasminogen pH 7.2, 37°C, two-chain form of mutant F423A, presence of soluble fibrin Homo sapiens
3.4.21.68 0.13
-
 Lys-plasminogen pH 7.2, 37°C, mutant R275E, single-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.15
-
 Lys-plasminogen pH 7.2, 37°C, two-chain form of mutant S421E, P422E and P422G, single chain form of mutant L420E/R275E, P422A/R275E and F423E/R275E, presence of soluble fibrin Homo sapiens
3.4.21.68 0.17
-
 Lys-plasminogen pH 7.2, 37°C, two-chain form of mutant L420E and L420A, single chain form of mutant P422E/R275E, presence of soluble fibrin Homo sapiens
3.4.21.68 0.18
-
 Lys-plasminogen pH 7.2, 37°C, two-chain form of mutant F423E, single-chain form of mutant L420A/R275E, presence of soluble fibrin Homo sapiens
3.4.21.68 0.19
-
 Lys-plasminogen pH 7.2, 37°C, mutant S421G/R275E, single-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.2
-
 Lys-plasminogen pH 7.2, 37°C, mutant F423A/R275E, single-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 0.21
-
 Lys-plasminogen pH 7.2, 37°C, mutant P422G/R275E and S421E/R275E, single-chain form, presence of soluble fibrin Homo sapiens
3.4.21.68 2 8 spectrozyme tPA pH 7.2, 37°C, mutant F423E/R275E, single chain form Homo sapiens
3.4.21.68 3 6 spectrozyme tPA pH 7.2, 37°C, mutant S421G, two-chain form Homo sapiens
3.4.21.68 14
-
 spectrozyme tPA pH 7.2, 37°C, mutant P422G/R275E, single chain form Homo sapiens
3.4.21.68 23
-
 spectrozyme tPA pH 7.2, 37°C, mutant S421G/R275E, single chain form Homo sapiens
3.4.21.68 26
-
 spectrozyme tPA pH 7.2, 37°C, mutant S421E/R275E, single chain form Homo sapiens
3.4.21.68 27
-
 spectrozyme tPA pH 7.2, 37°C, mutant F423A/R275E, single chain form Homo sapiens
3.4.21.68 29
-
 spectrozyme tPA pH 7.2, 37°C, mutant L420E/R275E, single chain form Homo sapiens
3.4.21.68 31
-
 spectrozyme tPA pH 7.2, 37°C, mutant P422E/R275E, single chain form Homo sapiens
3.4.21.68 32
-
 spectrozyme tPA pH 7.2, 37°C, mutant P422A/R275E, single chain form Homo sapiens
3.4.21.68 33
-
 spectrozyme tPA pH 7.2, 37°C, mutant R275E, single chain form Homo sapiens
3.4.21.68 42
-
 spectrozyme tPA pH 7.2, 37°C, mutant L420A/R275E, single chain form Homo sapiens
3.4.21.68 45
-
 spectrozyme tPA pH 7.2, 37°C, mutant L420A, L420E, two-chain form Homo sapiens
3.4.21.68 46
-
 spectrozyme tPA pH 7.2, 37°C, mutant S421E, two-chain form Homo sapiens
3.4.21.68 47
-
 spectrozyme tPA pH 7.2, 37°C, wild-type t-PA, mutant P422A, two-chain form Homo sapiens
3.4.21.68 49
-
 spectrozyme tPA pH 7.2, 37°C, mutant L420A/R275E, two-chain form Homo sapiens
3.4.21.68 57
-
 spectrozyme tPA pH 7.2, 37°C, mutant F423E, two-chain form Homo sapiens
3.4.21.68 60
-
 spectrozyme tPA pH 7.2, 37°C, mutant P422E, F423A, two-chain form Homo sapiens