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Literature summary extracted from
- Prostasin is a novel human serine proteinase from seminal fluid. Purification, tissue distribution, and localization in prostate gland (1994), J. Biol. Chem., 269, 18843-18848.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.B6 | antipain | 50% inhibition at 0.0064 mM | Homo sapiens |  |
| 3.4.21.B6 | Aprotinin | 50% inhibition at 0.0000018 mM | Homo sapiens | |
| 3.4.21.B6 | benzamidine | 50% inhibition at 0.86 mM | Homo sapiens | |
| 3.4.21.B6 | leupeptin | 50% inhibition at 0.1 mM | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.B6 | 108 | - |
D-Pro-Phe-Arg 7-amido-4-methylcoumarin | pH 9, 25°C | Homo sapiens | |
| 3.4.21.B6 | 108 | - |
D-Pro-Phe-Arg 7-amido-4-methylcoumarin | D-Pro-Phe-Arg-7-amido-4-methylcoumarin | Homo sapiens | |
| 3.4.21.B6 | 255 | - |
D-Val-Leu-Arg-7-amido-4-methylcoumarin | pH 9, 25°C | Homo sapiens | |
| 3.4.21.B6 | 717 | - |
Z-Gly-Pro-Arg-7-amido-4-trifluoromethylcoumarin | pH 9, 25°C | Homo sapiens | |
| 3.4.21.B6 | 827 | - |
D-Phe-Phe-Arg 7-amido-4-methylcoumarin | pH 9, 25°C | Homo sapiens | |
| 3.4.21.B6 | 827 | - |
D-Phe-Phe-Arg 7-amido-4-methylcoumarin | D-Phe-Phe-Arg-7-amido-4-methylcoumarin | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B6 | 40000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.B6 | Protein + H2O | Homo sapiens | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.B6 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.B6 | homogeneity | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.B6 | bronchus | - |
Homo sapiens | - |
| 3.4.21.B6 | colon | - |
Homo sapiens | - |
| 3.4.21.B6 | kidney | - |
Homo sapiens | - |
| 3.4.21.B6 | liver | - |
Homo sapiens | - |
| 3.4.21.B6 | lung | - |
Homo sapiens | - |
| 3.4.21.B6 | additional information | no activity in brain, muscle, testis, ventricle, atrium and aorta | Homo sapiens | - |
| 3.4.21.B6 | pancreas | - |
Homo sapiens | - |
| 3.4.21.B6 | prostate gland | epithelial cell and duct | Homo sapiens | - |
| 3.4.21.B6 | salivary gland | - |
Homo sapiens | - |
| 3.4.21.B6 | seminal plasma | - |
Homo sapiens | - |
| 3.4.21.B6 | urine | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.B6 | benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-trifluoromethylcoumarin | - |
Homo sapiens | benzyloxycarbonyl-Gly-Pro-Arg + 7-amino-4-trifluoromethylcoumarin | - |
? | |
| 3.4.21.B6 | D-Phe-Phe-Arg 4-methylcoumarin 7-amide | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.B6 | D-Phe-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | D-Phe-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B6 | D-Pro-Phe-Arg 4-methylcoumarin 7-amide | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.B6 | D-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | D-Pro-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B6 | D-Val-Leu-Arg 4-methylcoumarin 7-amide | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.B6 | D-Val-Leu-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | D-Val-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B6 | Protein + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.B6 | Z-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Z-Gly-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.B6 | S01.159 | Merops ID | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.B6 | 0.068 | - |
D-Pro-Phe-Arg-7-amido-4-methylcoumarin | pH 9, 25°C | Homo sapiens | |
| 3.4.21.B6 | 0.105 | - |
D-Val-Leu-Arg-7-amido-4-methylcoumarin | pH 9, 25°C | Homo sapiens | |
| 3.4.21.B6 | 0.287 | - |
Z-Gly-Pro-Arg-7-amido-4-trifluoromethylcoumarin | pH 9, 25°C | Homo sapiens | |
| 3.4.21.B6 | 0.29 | - |
D-Phe-Phe-Arg-7-amido-4-methylcoumarin | pH 9, 25°C | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B6 | 9 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B6 | 8.5 | 9.5 | 50% activity at pH 8.5 and pH 9.5 | Homo sapiens |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.21.B6 | Homo sapiens | isoelectric focusing, pH gradient 3.5-10 | - |
4.5 |
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