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Literature summary extracted from

  • Murphy, G.; Segain, J.P.; O'Shea, M.; Cockett, M.; Ioannou, C.; Lefebvre, O.; Chambon, P.; Basset, P.
    The 28-kDa N-terminal domain of mouse stromelysin-3 has the general properties of a weak metalloproteinase (1993), J. Biol. Chem., 268, 15435-15441.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B3 expression of an inactive enzyme in Escherichia coli BL21(DE3), expression of a functional enzyme in NSO mouse myeloma cell line Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B3 1,10-phenanthroline complete inhibition Mus musculus
3.4.24.B3 EDTA complete inhibition Mus musculus
3.4.24.B3 matrix metalloproteinase inhibitor TIMP-1 complete inhibition, human Mus musculus
3.4.24.B3 additional information no inhibition by phosphoramidon and L-3-caroxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane, i.e. E-64 Mus musculus
3.4.24.B3 phenylmethylsulfonyl fluoride slight inhibition Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.B3 28000
-
 x * 28000, recombinant active N-terminal domain, SDS-PAGE, x * 58000, inactive recombinant enzyme expressed in Escherichia coli, SDS-PAGE Mus musculus
3.4.24.B3 58000
-
 x * 28000, recombinant active N-terminal domain, SDS-PAGE, x * 58000, inactive recombinant enzyme expressed in Escherichia coli, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B3 protein + H2O Mus musculus
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B3 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.B3 proteolytic modification recombinant protein from mouse myeloma cells is cleaved and loses its propeptide and the majority of its C-terminal domain, recombinant enzyme can be cleaved by trypsin to a 51 kDa stil latent enzyme form Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B3 recombinant enzyme from Escherichia coli and NSO mouse myeloma cells Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.B3 21
-
 purified recombinant latently active enzyme form Mus musculus
3.4.24.B3 99
-
 purifed recombinant active enzyme form Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B3 aggrecan + H2O 28 kDa N-terminal domain, cartilage proteoglycan encapsulated in polyacrylamide beads Mus musculus ?
-
 ?
3.4.24.B3 carboxymethylated transferrin + H2O 28 kDa N-terminal domain Mus musculus ?
-
 ?
3.4.24.B3 casein + H2O 28 kDa N-terminal domain Mus musculus ?
-
 ?
3.4.24.B3 collagen type IV + H2O only unfolded collagen, 28 kDa N-terminal domain Mus musculus ?
-
 ?
3.4.24.B3 Fibronectin + H2O 28 kDa N-terminal domain Mus musculus ?
-
 ?
3.4.24.B3 Gelatin + H2O 28 kDa N-terminal domain, low activity Mus musculus ?
-
 ?
3.4.24.B3 Laminin + H2O 28 kDa N-terminal domain Mus musculus ?
-
 ?
3.4.24.B3 additional information enzyme can activate the activity of trypsin-activated interstitial collagenase, no activation of prostromelysin-1, progelatinase A, progelatinase B, no activity with collagen type I Mus musculus ?
-
 ?
3.4.24.B3 protein + H2O
-
 Mus musculus peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.B3 ? x * 28000, recombinant active N-terminal domain, SDS-PAGE, x * 58000, inactive recombinant enzyme expressed in Escherichia coli, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B3 M10.007 Merops-ID Mus musculus
3.4.24.B3 matrix metalloproteinase-11
-
 Mus musculus
3.4.24.B3 MMP-11
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.B3 37
-
 assay at Mus musculus