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Literature summary extracted from

  • Nok, A.J.; Shuaibu, M.N.; Choudhry, M.K.; Oyebanjo, O.; Ibrahim, S.; Williams, S.
    N-Acetyl alpha-D-glucosaminidase from the venom of African puff adder (Bitis arietans) (2001), J. Biochem. Mol. Toxicol., 15, 221-227.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.50 1-thio-beta-D-N-acetylglucosamine competitive Bitis arietans
3.2.1.50 colombin active component of antivenom preparation from Aristolodia albida, competitive Bitis arietans
3.2.1.50 Hg2+ noncompetitive Bitis arietans
3.2.1.50 N-acetylglucosamine competitive Bitis arietans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.50 0.00055
-
 p-nitrophenyl-1-thio-N-acetylglucosamine
-
 Bitis arietans
3.2.1.50 0.00065
-
 chitobiose
-
 Bitis arietans
3.2.1.50 0.0008
-
 chitotriose
-
 Bitis arietans
3.2.1.50 0.001
-
 methylumbelliferyl-N-acetylglucosamine
-
 Bitis arietans
3.2.1.50 0.003
-
 chitotetraose
-
 Bitis arietans
3.2.1.50 0.0035
-
 methylumbelliferyl-N-acetylglucosamine-6-sulfate
-
 Bitis arietans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.50 51000
-
 2 * 51000, SDS-PAGE Bitis arietans
3.2.1.50 102000
-
 gel filtration Bitis arietans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.50 Bitis arietans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.50
-
 Bitis arietans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.50 venom
-
 Bitis arietans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.50 additional information
-
-
 Bitis arietans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.50 chitobiose + H2O
-
 Bitis arietans ?
-
 ?
3.2.1.50 chitotetraose + H2O
-
 Bitis arietans ?
-
 ?
3.2.1.50 chitotriose + H2O
-
 Bitis arietans ?
-
 ?
3.2.1.50 methylumbelliferyl-N-acetylglucosamine + H2O
-
 Bitis arietans methylumbelliferone + N-acetylglucosamine
-
 ?
3.2.1.50 methylumbelliferyl-N-acetylglucosamine-6-sulfate + H2O
-
 Bitis arietans methylumbelliferone + N-acetylglucosamine-6-sulfate
-
 ?
3.2.1.50 additional information swollen chitin and oligosaccharide above (GlcNAc)4 are not hydrolyzed by the enzyme Bitis arietans ?
-
 ?
3.2.1.50 p-nitrophenyl-1-thio-N-acetylglucosamine + H2O
-
 Bitis arietans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.50 dimer 2 * 51000, SDS-PAGE Bitis arietans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.50 NAGase
-
 Bitis arietans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.50 40
-
-
 Bitis arietans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.50 70
-
 10 min, complete inactivation Bitis arietans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.50 5.5
-
 hydrolysis of methylumbelliferyl-N-acetylglucosamine Bitis arietans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.50 0.25
-
 1-thio-beta-D-N-acetylglucosamine
-
 Bitis arietans
3.2.1.50 0.55
-
 Hg2+
-
 Bitis arietans
3.2.1.50 0.6
-
 colombin
-
 Bitis arietans
3.2.1.50 8
-
 N-acetylglucosamine
-
 Bitis arietans