Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, S.K.; Park, P.J.; Kim, J.B.; Shahidi, F.
    Purification and characterization of a collagenolytic protease from the filefish, Novoden modestrus (2002), J. Biochem. Mol. Biol., 35, 165-171.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.32 Cd2+ 0.06 mM, 29% inhibition Novoden modestrus
3.4.21.32 Cu2+ 0.06 mM, 26% inhibition Novoden modestrus
3.4.21.32 diisopropylfluorophosphate 0.2 mM, complete inhibition Novoden modestrus
3.4.21.32 EDTA 0.2 mM, 46% inhibition. 0.5 mM, 95% inhibition Novoden modestrus
3.4.21.32 Hg2+ 0.06 mM, 19% inhibition Novoden modestrus
3.4.21.32 His 0.2 mM, 51% inhibition. 0.7 nmM, 93% inhibition Novoden modestrus
3.4.21.32 Mn2+ 0.06 mM, 14% inhibition Novoden modestrus
3.4.21.32 Ni2+ 0.06 mM, 30% inhibition Novoden modestrus
3.4.21.32 Soybean trypsin inhibitor 0.2 mM, complete inhibition Novoden modestrus
3.4.21.32 tosyl-L-Leu-chloromethylketone 0.2 mM, complete inhibition Novoden modestrus
3.4.21.32 Zn2+ 0.06 mM, 36% inhibition Novoden modestrus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.32 Ba2+ 0.06 mM, activation to 119% of control Novoden modestrus
3.4.21.32 Ca2+ 0.06 mM, activation to 135% of control Novoden modestrus
3.4.21.32 K+ 0.06 mM, activation to 123% of control Novoden modestrus
3.4.21.32 Li+ 0.06 mM, activation to 111% of control Novoden modestrus
3.4.21.32 Mg2+ 0.06 mM, activation to 111% of control Novoden modestrus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.32 27000
-
 gel filtration Novoden modestrus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.32 Novoden modestrus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.32
-
 Novoden modestrus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.32 10550
-
-
 Novoden modestrus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.32 Collagen + H2O
-
 Novoden modestrus Hydrolyzed collagen
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.32 monomer 1 * 27000, SDS-PAGE Novoden modestrus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.32 55
-
 hydrolysis of collagen Novoden modestrus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.32 45 60 45°C: about 40% of maximal activity, 60°C: about 45% of maximal activity, hydrolysis of collagen Novoden modestrus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.32 7 8 hydrolysis of collagen Novoden modestrus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.32 6.5 8.5 pH 6.5: about 60% of maximal activity, pH 8.5: about 50% of maximal activity, hydrolysis of collagen Novoden modestrus