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Literature summary extracted from

  • Jo, Y.J.; Lee, S.W.; Jo, M.K.; Lee, J.W.; Kang, M.K.; Yoon, J.H.; Kim, S.
    Methionine analogue probes functionally important residues in active site of methionyl-tRNA synthetase (1999), J. Biochem. Mol. Biol., 32, 547-553.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.10 T10M natural mutant, 5% activity compared to the wild-type, complementation of an enzyme-deficient Escherichia coli strain, no inhibition by L-methionine hydroxamate Escherichia coli
6.1.1.10 Y15A natural mutant, very low residual activity, complementation of an enzyme-deficient Escherichia coli strain Escherichia coli
6.1.1.10 Y94H natural mutant, unstable, no complementation of an enzyme-deficient Escherichia coli strain Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.10 L-methionine hydroxamate substrate analogue, inhibition mechanism, no inhibition of mutant T10M Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.10 7.9
-
 ATP wild-type, pH 7.5, 37°C Escherichia coli
6.1.1.10 7.9
-
 L-methionine wild-type, pH 7.5, 37°C Escherichia coli
6.1.1.10 58
-
 ATP mutant T10M, pH 7.5, 37°C Escherichia coli
6.1.1.10 58
-
 L-methionine mutant T10M, pH 7.5, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.10 Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Escherichia coli
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.10 ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet active site structure and reaction mechanism involving Tyr15 and Thr10, Tyr94 is involved in maintaining the native enzyme conformation Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet formation of an methionine adenylate reaction intermediate Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 additional information enzyme also performs the ATP-diphosphate exchange reaction Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 Methionine translase
-
 Escherichia coli
6.1.1.10 Methionine--tRNA ligase
-
 Escherichia coli
6.1.1.10 Methionyl tRNA synthetase
-
 Escherichia coli
6.1.1.10 Methionyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.10 Methionyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
6.1.1.10 Methionyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.10 MetRS
-
 Escherichia coli
6.1.1.10 Synthetase, methionyl-transfer ribonucleate
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.10 3.4
-
 ATP mutant T10M, pH 7.5, 37°C Escherichia coli
6.1.1.10 3.4
-
 L-methionine mutant T10M, pH 7.5, 37°C Escherichia coli
6.1.1.10 5.6
-
 ATP wild-type, pH 7.5, 37°C Escherichia coli
6.1.1.10 5.6
-
 L-methionine wild-type, pH 7.5, 37°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.1.1.10 0.0196
-
 L-methionine hydroxamate pH 8.0 Escherichia coli