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Literature summary extracted from

  • Lee, S.G.; Hong, S.P.; Kwak, M.S.; Esaki, N.; Sung, M.H.
    Characterization of thermostable tyrosine phenol-lyase from an obligatory symbiotic thermophile, Symbiobacterium sp. SC-1 (1999), J. Biochem. Mol. Biol., 32, 480-485.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.99.2 0.19
-
 L-tyrosine pH 7.2, 60°C Symbiobacterium sp.
4.1.99.2 0.36
-
 S-(o-nitrophenyl)-L-cysteine pH 7.2, 60°C Symbiobacterium sp.
4.1.99.2 5.5
-
 S-methyl-L-cysteine pH 7.2, 60°C Symbiobacterium sp.
4.1.99.2 9.9
-
 3,4-dihydroxyphenyl-L-alanine pH 7.2, 60°C Symbiobacterium sp.
4.1.99.2 12
-
 beta-chloro-L-alanine pH 7.2, 60°C Symbiobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.2 52200
-
 4 * 52200, deduced from amino acid sequence Symbiobacterium sp.
4.1.99.2 202000
-
 gel filtration Symbiobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.2 Symbiobacterium sp.
-
 SC-1
-
4.1.99.2 Symbiobacterium sp. SC-1
-
 SC-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.2
-
 Symbiobacterium sp.

Storage Stability

EC Number Storage Stability Organism
4.1.99.2 stable against 0.2% sodium dodecylsulfate Symbiobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.2 3,4-dihydroxyphenyl-L-alanine
-
 Symbiobacterium sp. ?
-
 ?
4.1.99.2 beta-chloro-L-alanine + H2O 500% of activity compared to L-tyrosine Symbiobacterium sp. ?
-
 ?
4.1.99.2 beta-chloro-L-alanine + H2O 500% of activity compared to L-tyrosine Symbiobacterium sp. SC-1 ?
-
 ?
4.1.99.2 D-serine + H2O at 11% the rate of L-tyrosine Symbiobacterium sp. ?
-
 ?
4.1.99.2 D-tyrosine + H2O at 7% the rate of L-tyrosine Symbiobacterium sp. phenol + pyruvate + NH3
-
 ?
4.1.99.2 D-tyrosine + H2O at 7% the rate of L-tyrosine Symbiobacterium sp. SC-1 phenol + pyruvate + NH3
-
 ?
4.1.99.2 L-cysteine + H2O
-
 Symbiobacterium sp. ?
-
 ?
4.1.99.2 L-cysteine + H2O
-
 Symbiobacterium sp. SC-1 ?
-
 ?
4.1.99.2 L-serine + H2O
-
 Symbiobacterium sp. ?
-
 ?
4.1.99.2 L-tyrosine + H2O
-
 Symbiobacterium sp. phenol + pyruvate + NH3
-
 ?
4.1.99.2 L-tyrosine + H2O
-
 Symbiobacterium sp. SC-1 phenol + pyruvate + NH3
-
 ?
4.1.99.2 S-(o-nitrophenyl)-L-cysteine + H2O 250% of activity compared to L-tyrosine Symbiobacterium sp. ?
-
 ?
4.1.99.2 S-(o-nitrophenyl)-L-cysteine + H2O 250% of activity compared to L-tyrosine Symbiobacterium sp. SC-1 ?
-
 ?
4.1.99.2 S-methyl-L-cysteine + H2O 385% of activity compared to L-tyrosine Symbiobacterium sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.2 tetramer 4 * 52200, deduced from amino acid sequence Symbiobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.99.2 80
-
-
 Symbiobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.99.2 8
-
-
 Symbiobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.2 pyridoxal 5'-phosphate 1 mol per mol of subunit Symbiobacterium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.99.2 Symbiobacterium sp. isoelectric focusing
-
 4.9