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Literature summary extracted from

  • An, J.H.; Lee, G.Y.; Song, J.H.; Lee, D.W.; Kim, Y.S.
    Properties of malonyl-CoA decarboxylase from Rhizobium trifolii (1999), J. Biochem. Mol. Biol., 32, 414-418.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.9 expression in Escherichia coli Rhizobium leguminosarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.9 iodoacetamide 1 mM, 16% inhibition Rhizobium leguminosarum
4.1.1.9 N-ethylmaleimide 1 mM, 47% inhibition Rhizobium leguminosarum
4.1.1.9 p-chloromercuribenzoate 0.1 mM, 95.5% inhibition Rhizobium leguminosarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.9 0.47
-
 malonyl-CoA
-
 Rhizobium leguminosarum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.9 cytoplasm
-
 Rhizobium leguminosarum 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.9 51000
-
 4 * 53000, SDS-PAGE, 4 * 51000, calculated from the amino acid sequence Rhizobium leguminosarum
4.1.1.9 53000
-
 4 * 53000, SDS-PAGE, 4 * 51000, calculated from the amino acid sequence Rhizobium leguminosarum
4.1.1.9 208000
-
 field flow fractionation Rhizobium leguminosarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.9 Malonyl-CoA Rhizobium leguminosarum malonate metabolism Acetyl-CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.9 Rhizobium leguminosarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.9 recombinant enzyme Rhizobium leguminosarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.9 additional information
-
-
 Rhizobium leguminosarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.9 Malonyl-CoA primary substrate, higher specific activity than with methylmalonyl-CoA as substrate, Cys-214 is located at or near the active site and plays a critical role in catalysis Rhizobium leguminosarum Acetyl-CoA + CO2
-
 ?
4.1.1.9 Malonyl-CoA malonate metabolism Rhizobium leguminosarum Acetyl-CoA + CO2
-
 ?
4.1.1.9 methylmalonyl-CoA specific activity is 0.1% that of malonyl-CoA decarboxylation Rhizobium leguminosarum propionyl-CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.9 homotetramer 4 * 53000, SDS-PAGE, 4 * 51000, calculated from the amino acid sequence Rhizobium leguminosarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.9 30
-
 assay at Rhizobium leguminosarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.9 7
-
 assay at Rhizobium leguminosarum