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Literature summary extracted from

  • Yapeng, C.; Ningguo, G.; Xiulan, C.; Jing, Y.; Shijun, Q.; Shuzheng, Z.
    Rapid purification, characterization and substrate specificity of heparinase from a novel species of Sphingobacterium (2003), J. Biochem., 134, 365-371.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.7 Urea up to 1 M of urea Sphingobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.7 N-Acetylimidazole 80% inhibition at 1 mM concentration of inhibitor, indicates the necessity of tyrosine residues in the active site Sphingobacterium sp.
4.2.2.7 N-bromosuccinimide 30% inhibition at 1 mM concentration of inhibitor Sphingobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.7 0.042
-
 N-acetyl-de-O-sulfated heparin pH 7.4, 37ºC Sphingobacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.7 periplasm
-
 Sphingobacterium sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.7 70100
-
 1 * 70100, SDS-PAGE Sphingobacterium sp.
4.2.2.7 75670
-
 mass spectrometry Sphingobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.7 Sphingobacterium sp.
-
 novel species isolated from the effluents of a food mill
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.7 three steps osmotic shock procedure, followed by chromatography on SP-Sepharose and Source 30S resin Sphingobacterium sp.

Storage Stability

EC Number Storage Stability Organism
4.2.2.7 unstable if stored at 4ºC, stability is increased with bovine serum albumin addition Sphingobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.7 de-N-sulfated heparin 8% activity compared to heparin Sphingobacterium sp. ?
-
 ?
4.2.2.7 deaminated heparin 54% activity compared to heparin Sphingobacterium sp. ?
-
 ?
4.2.2.7 heparan sulfate bovine or porcine Sphingobacterium sp. ?
-
 ?
4.2.2.7 additional information chondroitin sulfate A,B or C, and hyaluronic acid can not act as substrates Sphingobacterium sp. ?
-
 ?
4.2.2.7 N-acetyl heparin 78% activity compared to heparin Sphingobacterium sp. ?
-
 ?
4.2.2.7 N-acetyl-de-O-sulfated heparin 250% activity compared to heparin Sphingobacterium sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.7 monomer 1 * 70100, SDS-PAGE Sphingobacterium sp.