BRENDA - Enzyme Database

Crystal structure of Enterococcus hirae enolase at 2.8 A resolution

Hosaka, T.; Meguro, T.; Yamato, I.; Shirakihara, Y.; J. Biochem. 133, 817-823 (2003)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.11
using the hanging drop method followed by recrystallization under the same conditions, X-ray structure solved by molecular replacement
Enterococcus hirae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
two magnesium ions per subunit are required for catalytic activity
Enterococcus hirae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46220
-
calculated from amino-acid sequence
Enterococcus hirae
4.2.1.11
46620
-
determined by mass spectrometry
Enterococcus hirae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Enterococcus hirae
Q8GR70
gram-positive bacteria, ATCC9790
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
partial, using ammonium sulfate fractionation and gel filtration chromatography
Enterococcus hirae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
124
-
pH 7.5, 22║C
Enterococcus hirae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
651805
Enterococcus hirae
phosphoenolpyruvate
-
651805
Enterococcus hirae
r
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.11
using the hanging drop method followed by recrystallization under the same conditions, X-ray structure solved by molecular replacement
Enterococcus hirae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
two magnesium ions per subunit are required for catalytic activity
Enterococcus hirae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46220
-
calculated from amino-acid sequence
Enterococcus hirae
4.2.1.11
46620
-
determined by mass spectrometry
Enterococcus hirae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
partial, using ammonium sulfate fractionation and gel filtration chromatography
Enterococcus hirae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
124
-
pH 7.5, 22║C
Enterococcus hirae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
651805
Enterococcus hirae
phosphoenolpyruvate
-
651805
Enterococcus hirae
r