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Literature summary extracted from

  • Kazuoka, T.; Masuda, Y.; Oikawa, T.; Soda, K.
    Thermostable aspartase from a marine psychrophile, Cytophaga sp. KUC-1: molecular characterization and primary structure (2003), J. Biochem., 133, 51-58.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.1 Ba2+ 2 mM, 98% inhibition Cytophaga sp.
4.3.1.1 Hg2+ 2 mM, 98% inhibition Cytophaga sp.
4.3.1.1 iodoacetate 1 mM, 94% inhibition Cytophaga sp.
4.3.1.1 K+ 2 mM, 98% inhibition Cytophaga sp.
4.3.1.1 N-ethylmaleimide 1 mM, 100% inhibition Cytophaga sp.
4.3.1.1 Na+ 2 mM, 98% inhibition Cytophaga sp.
4.3.1.1 Ni2+ 2 mM, 98% inhibition Cytophaga sp.
4.3.1.1 p-chloromercuribenzoate 0.1 mM, 100% inhibition Cytophaga sp.
4.3.1.1 Semicarbazide hydrochloride 1 mM, 30% inhibition Cytophaga sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.1 0.797
-
 fumarate 0.1 mM, 100% inhibition Cytophaga sp.
4.3.1.1 1.09
-
 L-aspartate pH 7.0, 55°C Cytophaga sp.
4.3.1.1 25.2
-
 NH3 0.1 mM, 100% inhibition Cytophaga sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.1 Ca2+ activation at neutral pH Cytophaga sp.
4.3.1.1 Mg2+ activation at neutral pH Cytophaga sp.
4.3.1.1 Mn2+ activation at neutral pH Cytophaga sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.1 51000
-
 4 * 51000, SDS-PAGE Cytophaga sp.
4.3.1.1 192000
-
 gel filtration Cytophaga sp.

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.1 Cytophaga sp.
-
 KUC-1
-
4.3.1.1 Cytophaga sp. KUC-1
-
 KUC-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.1
-
 Cytophaga sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.1 301
-
 pH 8.5, 30°C Cytophaga sp.

Storage Stability

EC Number Storage Stability Organism
4.3.1.1 -20°C, pH 7.0, 2% 2-mercaptoethanol, several months, no loss of activity Cytophaga sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.1 fumarate + hydroxylamine at 64% the rate of NH3 Cytophaga sp. L-aspartate
-
 ?
4.3.1.1 fumarate + hydroxylamine at 64% the rate of NH3 Cytophaga sp. KUC-1 L-aspartate
-
 ?
4.3.1.1 fumarate + NH3
-
 Cytophaga sp. L-aspartate
-
 ?
4.3.1.1 fumarate + NH3
-
 Cytophaga sp. KUC-1 L-aspartate
-
 ?
4.3.1.1 L-aspartate
-
 Cytophaga sp. fumarate + NH3
-
 r
4.3.1.1 L-aspartate
-
 Cytophaga sp. KUC-1 fumarate + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
4.3.1.1 homotetramer 4 * 51000, SDS-PAGE Cytophaga sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.1 55
-
-
 Cytophaga sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.3.1.1 15 45
-
 Cytophaga sp.
4.3.1.1 50
-
 60 min, 80% residual activity Cytophaga sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.1 7.5
-
 absence of Mg2+ Cytophaga sp.
4.3.1.1 8.5
-
 presence of Mg2+ Cytophaga sp.