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Literature summary extracted from

  • Muta, Y.; Inouye, K.
    Inhibitory effects of alcohols on thermolysin activity as examined using a fluorescent substrate (2002), J. Biochem., 132, 945-951.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.27 three times crystallized and lyophilized preparation, Lot T5CB491 Bacillus thermoproteolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.27 1-butanol
-
 Bacillus thermoproteolyticus
3.4.24.27 1-Pentanol
-
 Bacillus thermoproteolyticus
3.4.24.27 1-propanol
-
 Bacillus thermoproteolyticus
3.4.24.27 2-butanol
-
 Bacillus thermoproteolyticus
3.4.24.27 2-methyl-1-propanol
-
 Bacillus thermoproteolyticus
3.4.24.27 2-propanol
-
 Bacillus thermoproteolyticus
3.4.24.27 ethanol
-
 Bacillus thermoproteolyticus
3.4.24.27 methanol
-
 Bacillus thermoproteolyticus
3.4.24.27 tert-amyl alcohol
-
 Bacillus thermoproteolyticus
3.4.24.27 tert-amylalcohol
-
 Bacillus thermoproteolyticus
3.4.24.27 tert-butyl alcohol
-
 Bacillus thermoproteolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.27 Ca2+ 4 calcium ions required for structural stability Bacillus thermoproteolyticus
3.4.24.27 NaCl induces activation Bacillus thermoproteolyticus
3.4.24.27 Zn2+ required for enzyme activity Bacillus thermoproteolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.27 Bacillus thermoproteolyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.27 (7-methoxycoumarin-4-yl) acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 + H2O fluorescent substrate Bacillus thermoproteolyticus ?
-
 ?
3.4.24.27 N-[3-(2-furyl)acryloyl]-Gly-L-Leu-NH2 + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.27 35
-
 1-Pentanol pH 7.5, 25°C Bacillus thermoproteolyticus
3.4.24.27 38
-
 1-propanol pH 7.5, 25°C Bacillus thermoproteolyticus
3.4.24.27 40
-
 2-butanol pH 7.5, 25°C Bacillus thermoproteolyticus
3.4.24.27 41
-
 2-methyl-1-propanol pH 7.5, 25°C Bacillus thermoproteolyticus
3.4.24.27 50
-
 1-butanol pH 7.5, 25°C Bacillus thermoproteolyticus
3.4.24.27 65
-
 1-propanol pH 7.5, 25°C Bacillus thermoproteolyticus
3.4.24.27 100
-
 ethanol pH 7.5, 25°C Bacillus thermoproteolyticus
3.4.24.27 110
-
 tert-amylalcohol pH 7.5, 25°C Bacillus thermoproteolyticus
3.4.24.27 190
-
 tert-butyl alcohol pH 7.5, 25°C Bacillus thermoproteolyticus
3.4.24.27 430
-
 methanol pH 7.5, 25°C Bacillus thermoproteolyticus