Any feedback?
Literature summary extracted from
- Platelet-activating factor acetylhydrolase (PAF-AH) (2002), J. Biochem., 131, 635-640.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.47 | - |
Bos taurus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.1.47 | crystal structure determination and analysis of the alpha1 subunit in complex with acetate at 1.7 A resolution | Bos taurus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.47 | cytosol | isozyme II | Bos taurus | 5829 | - |
| 3.1.1.47 | intracellular | - |
Bos taurus | 5622 | - |
| 3.1.1.47 | membrane | isozyme II | Bos taurus | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.47 | 40000 | - |
x * 40000, isozyme II, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.47 | 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Mus musculus | i.e. platelet-activation factor | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? |  |
| 3.1.1.47 | 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Homo sapiens | i.e. platelet-activation factor | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 3.1.1.47 | 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Rattus norvegicus | i.e. platelet-activation factor | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 3.1.1.47 | 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Bos taurus | biological inactivation of the platelet-activation factor by hydrolysis of the acetyl group at sn-2 position which is essential for activity of PAF | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 3.1.1.47 | additional information | Mus musculus | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | ? | - |
? | |
| 3.1.1.47 | additional information | Homo sapiens | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | ? | - |
? | |
| 3.1.1.47 | additional information | Rattus norvegicus | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | ? | - |
? | |
| 3.1.1.47 | additional information | Bos taurus | one of the physiological roles of isozyme II is to diversify the biological function of PAF by producing different lipid mediators such as analogues of PAF and C2-ceramide, mutations of the gene encoding the enzyme lead to severe diseases, e.g. brain impairment, mental retardation, or lymphoma development | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.47 | Bos taurus | - |
isozymes I and II | - |
| 3.1.1.47 | Homo sapiens | - |
- |
- |
| 3.1.1.47 | Mus musculus | - |
- |
- |
| 3.1.1.47 | Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.1.47 | additional information | isozyme II contains a myristoylation signal at the N-terminus | Bos taurus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.47 | isozyme II from liver, and isozyme I from brain | Bos taurus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.1.1.47 | 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate | mechanism | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.47 | brain | neonatal and adult, isozyme I | Bos taurus | - |
| 3.1.1.47 | glial cell | - |
Bos taurus | - |
| 3.1.1.47 | liver | isozyme II | Bos taurus | - |
| 3.1.1.47 | additional information | distribution of hetero- and homdimer of the alpha-subunit | Bos taurus | - |
| 3.1.1.47 | neuron | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.47 | 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Mus musculus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 3.1.1.47 | 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Homo sapiens | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 3.1.1.47 | 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Rattus norvegicus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 3.1.1.47 | 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Bos taurus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 3.1.1.47 | 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | biological inactivation of the platelet-activation factor by hydrolysis of the acetyl group at sn-2 position which is essential for activity of PAF | Bos taurus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 3.1.1.47 | additional information | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | Mus musculus | ? | - |
? | |
| 3.1.1.47 | additional information | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | Homo sapiens | ? | - |
? | |
| 3.1.1.47 | additional information | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | Rattus norvegicus | ? | - |
? | |
| 3.1.1.47 | additional information | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | Bos taurus | ? | - |
? | |
| 3.1.1.47 | additional information | one of the physiological roles of isozyme II is to diversify the biological function of PAF by producing different lipid mediators such as analogues of PAF and C2-ceramide, mutations of the gene encoding the enzyme lead to severe diseases, e.g. brain impairment, mental retardation, or lymphoma development | Bos taurus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.47 | ? | x * 40000, isozyme II, SDS-PAGE | Bos taurus |
| 3.1.1.47 | More | isozyme I exists of alpha1 and alpha2 subunits, catalytic subunits, tertiary structure, as well as of a beta-subunit, the beta-subunit is a regulatory one, isozyme II is a monomer | Mus musculus |
| 3.1.1.47 | More | isozyme I exists of alpha1 and alpha2 subunits, catalytic subunits, tertiary structure, as well as of a beta-subunit, the beta-subunit is a regulatory one, isozyme II is a monomer | Homo sapiens |
| 3.1.1.47 | More | isozyme I exists of alpha1 and alpha2 subunits, catalytic subunits, tertiary structure, as well as of a beta-subunit, the beta-subunit is a regulatory one, isozyme II is a monomer | Rattus norvegicus |
| 3.1.1.47 | More | isozyme I exists of alpha1 and alpha2 subunits, catalytic subunits, tertiary structure, as well as of a beta-subunit, the beta-subunit is a regulatory one, isozyme II is a monomer | Bos taurus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.47 | PAF-AH | - |
Mus musculus |
| 3.1.1.47 | PAF-AH | - |
Homo sapiens |
| 3.1.1.47 | PAF-AH | - |
Rattus norvegicus |
| 3.1.1.47 | PAF-AH | - |
Bos taurus |
| 3.1.1.47 | platelet-activating factor acetylhydrolase | - |
Mus musculus |
| 3.1.1.47 | platelet-activating factor acetylhydrolase | - |
Homo sapiens |
| 3.1.1.47 | platelet-activating factor acetylhydrolase | - |
Rattus norvegicus |
| 3.1.1.47 | platelet-activating factor acetylhydrolase | - |
Bos taurus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
