EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.14.1 | additional information | enzyme is induced during nitrogen starvation | Schizosaccharomyces pombe | |
3.1.14.1 | protease isp6+ | strongly dependent on, gene isp6+ encodes a protease, which is involved in RNA-degrading enzyme activity, an isp6+-deficient mutant is also devoid of RNA-degrading enzyme activity | Schizosaccharomyces pombe |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.14.1 | overexpression of wild-type enzyme in Schizosaccharomyces pombe | Schizosaccharomyces pombe |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.14.1 | additional information | construction of a enzyme null-mutant pnu1DELTA, pnu1+ is dispensable for mitochondrial function and not essential for cell growth | Schizosaccharomyces pombe |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.14.1 | EDTA | recoverage by either Mg2+, Cl-, or CaCl2 | Schizosaccharomyces pombe |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.14.1 | mitochondrion | - |
Schizosaccharomyces pombe | 5739 | - |
3.1.14.1 | soluble | - |
Schizosaccharomyces pombe | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.14.1 | Mg2+ | - |
Schizosaccharomyces pombe |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.14.1 | 38000 | - |
x * 38000, SDS-PAGE | Schizosaccharomyces pombe |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.14.1 | RNA + H2O | Schizosaccharomyces pombe | - |
nucleoside 3'-phosphates | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.14.1 | Schizosaccharomyces pombe | - |
gene pnu1+ | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.14.1 | recombinant wild-type enzyme from overexpressing cells | Schizosaccharomyces pombe |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.14.1 | DNA + H2O | pUC18 or pSP1 | Schizosaccharomyces pombe | deoxynucleoside 3'-phosphates | - |
? | |
3.1.14.1 | additional information | the enzyme is not essential for degradation of 26S and 17S rRNA | Schizosaccharomyces pombe | ? | - |
? | |
3.1.14.1 | RNA + H2O | - |
Schizosaccharomyces pombe | nucleoside 3'-phosphates | - |
? | |
3.1.14.1 | RNA + H2O | total RNA from Schizosaccharomyces pombe | Schizosaccharomyces pombe | nucleoside 3'-phosphates | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.14.1 | ? | x * 38000, SDS-PAGE | Schizosaccharomyces pombe |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.14.1 | nuclease 1 | - |
Schizosaccharomyces pombe |
3.1.14.1 | RNA-degrading enzyme | - |
Schizosaccharomyces pombe |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.14.1 | 30 | - |
assay at | Schizosaccharomyces pombe |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.14.1 | 7.6 | - |
assay at | Schizosaccharomyces pombe |