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Literature summary extracted from

  • Nakashima, A.; Yoshida, M.; Nakayama, K.; Kato-Furuno, A.; Ueno, M.; Ushimaru, T.; Uritani, M.
    Genes for a nuclease and a protease are involved in the drastic decrease in cellular RNA amount in fission yeast cells during nitrogen starvation (2002), J. Biochem., 131, 391-398.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.14.1 additional information enzyme is induced during nitrogen starvation Schizosaccharomyces pombe
3.1.14.1 protease isp6+ strongly dependent on, gene isp6+ encodes a protease, which is involved in RNA-degrading enzyme activity, an isp6+-deficient mutant is also devoid of RNA-degrading enzyme activity Schizosaccharomyces pombe

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.14.1 overexpression of wild-type enzyme in Schizosaccharomyces pombe Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
3.1.14.1 additional information construction of a enzyme null-mutant pnu1DELTA, pnu1+ is dispensable for mitochondrial function and not essential for cell growth Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.14.1 EDTA recoverage by either Mg2+, Cl-, or CaCl2 Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.14.1 mitochondrion
-
Schizosaccharomyces pombe 5739
-
3.1.14.1 soluble
-
Schizosaccharomyces pombe
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.14.1 Mg2+
-
Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.14.1 38000
-
x * 38000, SDS-PAGE Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.14.1 RNA + H2O Schizosaccharomyces pombe
-
nucleoside 3'-phosphates
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.14.1 Schizosaccharomyces pombe
-
gene pnu1+
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.14.1 recombinant wild-type enzyme from overexpressing cells Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.14.1 DNA + H2O pUC18 or pSP1 Schizosaccharomyces pombe deoxynucleoside 3'-phosphates
-
?
3.1.14.1 additional information the enzyme is not essential for degradation of 26S and 17S rRNA Schizosaccharomyces pombe ?
-
?
3.1.14.1 RNA + H2O
-
Schizosaccharomyces pombe nucleoside 3'-phosphates
-
?
3.1.14.1 RNA + H2O total RNA from Schizosaccharomyces pombe Schizosaccharomyces pombe nucleoside 3'-phosphates
-
?

Subunits

EC Number Subunits Comment Organism
3.1.14.1 ? x * 38000, SDS-PAGE Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
3.1.14.1 nuclease 1
-
Schizosaccharomyces pombe
3.1.14.1 RNA-degrading enzyme
-
Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.14.1 30
-
assay at Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.14.1 7.6
-
assay at Schizosaccharomyces pombe