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Literature summary extracted from

  • Takahashi, S.; Takahashi, K.; Kaneko, T.; Ogasawara, H.; Shindo, S.; Saito, K.; Kawamura, Y.
    Identification of functionally important cysteine residues of the human renin-binding protein as the enzyme N-acetyl-D-glucosamine 2-epimerase (2001), J. Biochem., 129, 529-535.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.8 construction of several C-terminal deletion and multi-cysteine/serine mutants and expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.8 C125S no loss of activity compared to wild-type enzyme Homo sapiens
5.1.3.8 C125S/C210S mutant enzyme shows 54.8% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C125S/C210S/C239S mutant enzyme shows 49.3% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C125S/C210S/C239S/C203S mutant enzyme shows 28.7% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C125S/C210S/C239S/C203S/C386S mutant enzyme shows 23.8% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C125S/C210S/C302S/C390S no activity detected Homo sapiens
5.1.3.8 C125S/C386S mutant enzyme shows 68.7% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C125S/C390S mutant enzyme shows 5.1% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C210S/C386S mutant enzyme shows 88.7% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C210S/C390S mutant enzyme shows 30.9% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C239S/C386S mutant enzyme shows 116% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C239S/C390S mutant enzyme shows 27.8% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C302S/C386S mutant enzyme shows 65.8% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C302S/C390S mutant enzyme shows 7.4% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C386S no loss of activity compared to wild-type enzyme Homo sapiens
5.1.3.8 C390S no loss of activity compared to wild-type enzyme Homo sapiens
5.1.3.8 C41S no loss of activity compared to wild-type enzyme Homo sapiens
5.1.3.8 C41S/C125S mutant enzyme shows 17.7% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C41S/C125S/C210S mutant enzyme shows 28.1% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C41S/C125S/C210S/C239S mutant enzyme shows 9.7% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C41S/C125S/C210S/C239S/C302S no activity detected Homo sapiens
5.1.3.8 C41S/C125S/C210S/C239S/C302S/C386S no activity detected Homo sapiens
5.1.3.8 C41S/C125S/C210S/C239S/C302S/C390S no activity detected Homo sapiens
5.1.3.8 C41S/C386S mutant enzyme shows 0.7% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C41S/C390S no activity detected Homo sapiens
5.1.3.8 C66S no loss of activity compared to wild-type enzyme Homo sapiens
5.1.3.8 C66S/C125S mutant enzyme shows 39.4% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C66S/C125S/C210S mutant enzyme shows 23.9% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C66S/C125S/C210S/C239S mutant enzyme shows 58.4% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C66S/C125S/C210S/C239S/C302S mutant enzyme shows 15.5% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C66S/C125S/C210S/C302S/C386S no activity detected Homo sapiens
5.1.3.8 C66S/C125S/C210S/C302S/C390S no activity detected Homo sapiens
5.1.3.8 C66S/C386S mutant enzyme shows 113% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 C66S/C390S mutant enzyme shows 14.4% of the activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 DELTA380-417 mutant enzyme has no activity Homo sapiens
5.1.3.8 DELTA386-417 mutant enzyme has no activity Homo sapiens
5.1.3.8 DELTA390-417 mutant enzyme has no activity Homo sapiens
5.1.3.8 DELTA400-417 C-terminal deletion mutant has approximately 50% activity relative to the wild-type enzyme Homo sapiens
5.1.3.8 additional information mutational analysis of multi-cysteine/serine mutants reveals that Cys41 and Cys390 are critical for the activity or stabilization of the enzyme, while cysteine residues in the middle of the enzyme, Cys125, Cys210, Cys239, and Cys302 have no essential function in relation to the activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.8 Homo sapiens
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Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.8 N-acetyl-D-mannosamine
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Homo sapiens N-acetyl-D-glucosamine
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?