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Literature summary extracted from

  • Imamura, S.; Kitaura, S.
    Purification and characterization of a monoacylglycerol lipase from the moderately thermophilic Bacillus sp. H-257 (2000), J. Biochem., 127, 419-425.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.23 deoxycholate 17% inhibition at 5 mM Bacillus sp. (in: Bacteria)
3.1.1.23 Triton X-100 10% inhibition at 0.25% Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.23 0.059
-
2-monooleoylglycerol pH 7.3, 37°C Bacillus sp. (in: Bacteria)
3.1.1.23 0.083
-
1-monooleoylglycerol pH 7.3, 37°C Bacillus sp. (in: Bacteria)
3.1.1.23 0.14
-
1-monolauroylglycerol pH 7.3, 37°C Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.23 Bacillus sp. (in: Bacteria)
-
-
-
3.1.1.23 Bacillus sp. (in: Bacteria) H-257
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.23
-
Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.23 121.1
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.23 1-monolauroylglycerol + H2O best substrate Bacillus sp. (in: Bacteria) lauric acid + glycerol
-
?
3.1.1.23 1-monolauroylglycerol + H2O best substrate Bacillus sp. (in: Bacteria) H-257 lauric acid + glycerol
-
?
3.1.1.23 1-monooleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) oleic acid + glycerol
-
?
3.1.1.23 1-monooleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) H-257 oleic acid + glycerol
-
?
3.1.1.23 2-monooleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) oleic acid + glycerol
-
?
3.1.1.23 2-monooleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) H-257 oleic acid + glycerol
-
?
3.1.1.23 monolinoleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) linoic acid + glycerol
-
?
3.1.1.23 monolinoleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) H-257 linoic acid + glycerol
-
?
3.1.1.23 monomyristoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) myristic acid + glycerol
-
?
3.1.1.23 monostearoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) stearic acid + glycerol
-
?
3.1.1.23 monostearoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) H-257 stearic acid + glycerol
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.23 75
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.23 60
-
stable for 10 min Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.23 6 8
-
Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.23 Bacillus sp. (in: Bacteria) isoelectric focusing, pH gradient 3.5-10 4.8 4.7