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Literature summary extracted from

  • Tanaka, T.; Matsuzawa, H.; Ohta, T.
    Identification and designing of the S3 site of aqualysin I, a thermophilic subtilisin-related serine protease (1999), J. Biochem., 125, 1016-1021.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.111
-
 Thermus aquaticus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.111 G131D reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus
3.4.21.111 G131H reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus
3.4.21.111 G131K reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus
3.4.21.111 S102E reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus
3.4.21.111 S102H reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus
3.4.21.111 S102K reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.111 0.044
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 0.15
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus
3.4.21.111 0.24
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
3.4.21.111 0.34
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
3.4.21.111 0.49
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131D mutant Thermus aquaticus
3.4.21.111 0.51
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
3.4.21.111 0.52
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
3.4.21.111 1
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131D mutant Thermus aquaticus
3.4.21.111 1.1
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
3.4.21.111 1.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 1.2
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 1.7
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus
3.4.21.111 1.8
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
3.4.21.111 1.9
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
3.4.21.111 2
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
3.4.21.111 2.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131D mutant Thermus aquaticus
3.4.21.111 2.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
3.4.21.111 2.3
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
3.4.21.111 3.8
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
3.4.21.111 7.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
3.4.21.111 10
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.111 Thermus aquaticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.111 N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.111 0.11
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
3.4.21.111 0.11
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
3.4.21.111 0.5
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
3.4.21.111 1.1
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
3.4.21.111 1.2
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 1.5
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
3.4.21.111 1.6
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
3.4.21.111 2.4
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131D mutant Thermus aquaticus
3.4.21.111 2.9
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus
3.4.21.111 5.4
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
3.4.21.111 7.3
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
3.4.21.111 7.9
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131D mutant Thermus aquaticus
3.4.21.111 8.5
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus
3.4.21.111 8.9
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
3.4.21.111 9
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
3.4.21.111 11
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 25
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
3.4.21.111 33
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
3.4.21.111 54
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
3.4.21.111 144
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus