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Literature summary extracted from

  • Nonaka, T.; Hashimoto, Y.; Takio, K.
    Kinetic characterization of lysine-specific metalloendopeptidases from Grifola frondosa and Pleurotus ostreatus fruiting bodies (1998), J. Biochem., 124, 157-162.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.20 2,4-dinitrophenyl-Ala-Thr-D-lysine-Leu-Ser-Try
-
Grifola frondosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.20 0.0088
-
2,4-dinitrophenyl-DATKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 0.0111
-
2,4-dinitrophenyl-APKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 0.0114
-
2,4-dinitrophenyl-STATKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 0.0116
-
2,4-dinitrophenyl-ADKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 0.0121
-
2,4-dinitrophenyl-ATKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 0.0133
-
2,4-dinitrophenyl-ATKLSTSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 0.0152
-
2,4-dinitrophenyl-ARKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 0.0155
-
2,4-dinitrophenyl-DADKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 0.0177
-
2,4-dinitrophenyl-ATKRSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 0.0249
-
2,4-dinitrophenyl-ATKDSW pH 9.5, 37°C Grifola frondosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.20 Co2+ can substitute for Zn2+, enhances activity Pleurotus ostreatus
3.4.24.20 Zn2+ zinc-metalloendopetidase Grifola frondosa
3.4.24.20 Zn2+ zinc-metalloendopetidase Pleurotus ostreatus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.20 Grifola frondosa P81054 edible mushroom
-
3.4.24.20 Pleurotus ostreatus P81055 edible mushroom
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.20
-
Grifola frondosa
3.4.24.20
-
Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.20 2,4-dinitrophenyl-ADKLSW + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 2,4-dinitrophenyl-APKLSW + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 2,4-dinitrophenyl-APKLSW + H2O
-
Grifola frondosa 2,4-dinitrophenyl-Ala-Pro + KLSW
-
?
3.4.24.20 2,4-dinitrophenyl-ARKLSW + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 2,4-dinitrophenyl-ARKLSW + H2O
-
Grifola frondosa 2,4-dinitrophenyl-Ala-Arg + KLSW
-
?
3.4.24.20 2,4-dinitrophenyl-ATKDSW + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 2,4-dinitrophenyl-ATKDSW + H2O
-
Grifola frondosa 2,4-dinitrophenyl-Ala-Thr + KDSW
-
?
3.4.24.20 2,4-dinitrophenyl-ATKLSTSW + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 2,4-dinitrophenyl-ATKLSTSW + H2O
-
Grifola frondosa 2,4-dinitrophenyl-Ala-Thr + KLSTSW
-
?
3.4.24.20 2,4-dinitrophenyl-ATKLSW + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 2,4-dinitrophenyl-ATKLSW + H2O
-
Grifola frondosa 2,4-dinitrophenyl-Ala-Thr + KLSW
-
?
3.4.24.20 2,4-dinitrophenyl-ATKRSW + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 2,4-dinitrophenyl-ATKRSW + H2O
-
Grifola frondosa 2,4-dinitrophenyl-Ala-Thr + KRSW
-
?
3.4.24.20 2,4-dinitrophenyl-DADKLSW + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 2,4-dinitrophenyl-DADKLSW + H2O
-
Grifola frondosa 2,4-dinitrophenyl-DAD + KLSW
-
?
3.4.24.20 2,4-dinitrophenyl-DATKLSW + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 2,4-dinitrophenyl-DATKLSW + H2O
-
Grifola frondosa 2,4-dinitrophenyl-DAT + KLSW
-
?
3.4.24.20 2,4-dinitrophenyl-Ser-Thr-Ala-Thr-Lys-Leu-Ser-Trp + H2O fluorescent peptide substrate Grifola frondosa ?
-
?
3.4.24.20 2,4-dinitrophenyl-Ser-Thr-Ala-Thr-Lys-Leu-Ser-Trp + H2O fluorescent peptide substrate Pleurotus ostreatus ?
-
?
3.4.24.20 2,4-dinitrophenyl-STATKLSW + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 2,4-dinitrophenyl-STATKLSW + H2O
-
Grifola frondosa 2,4-dinitrophenyl-STAT + KLSW
-
?
3.4.24.20 azocasein + H2O
-
Grifola frondosa ?
-
?
3.4.24.20 azocasein + H2O
-
Pleurotus ostreatus ?
-
?
3.4.24.20 additional information peptides with aspartic acid adjacent to the Lys residue are poor substrates, 2,4-dinitrophenyl-Thr-Lys-Trp and peptides with substitution of L-Arg, L-ornithine, or D-Lys are no substrates Grifola frondosa ?
-
?
3.4.24.20 additional information peptides with aspartic acid adjacent to the Lys residue are poor substrates, 2,4-dinitrophenyl-Thr-Lys-Trp and peptides with substitution of L-Arg, L-ornithine, or D-Lys are no substrates Pleurotus ostreatus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.20 GFMEP
-
Grifola frondosa
3.4.24.20 POMEP
-
Pleurotus ostreatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.20 0.12
-
2,4-dinitrophenyl-DADKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 0.25
-
2,4-dinitrophenyl-ATKDSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 0.99
-
2,4-dinitrophenyl-ATKLSTSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 1.3
-
2,4-dinitrophenyl-ADKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 3
-
2,4-dinitrophenyl-ATKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 3.7
-
2,4-dinitrophenyl-ATKRSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 5.1
-
2,4-dinitrophenyl-DATKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 5.5
-
2,4-dinitrophenyl-APKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 17.6
-
2,4-dinitrophenyl-ATKLSTSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 21
-
2,4-dinitrophenyl-STATKLSW pH 9.5, 37°C Grifola frondosa
3.4.24.20 54.7
-
2,4-dinitrophenyl-ARKLSW pH 9.5, 37°C Grifola frondosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.20 5.6
-
azocasein hydrolysis Pleurotus ostreatus
3.4.24.20 8.5
-
rather active with fluorogenic peptide substrates than at pH 5.0 Pleurotus ostreatus
3.4.24.20 9.5
-
-
Grifola frondosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.20 6 10.5 exhibits more than 50% of the maximal activity over this range Grifola frondosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.20 0.0399
-
2,4-dinitrophenyl-Ala-Thr-D-lysine-Leu-Ser-Try pH 9.5, 37°C Grifola frondosa