EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.20 | 2,4-dinitrophenyl-Ala-Thr-D-lysine-Leu-Ser-Try | - |
Grifola frondosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.20 | 0.0088 | - |
2,4-dinitrophenyl-DATKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 0.0111 | - |
2,4-dinitrophenyl-APKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 0.0114 | - |
2,4-dinitrophenyl-STATKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 0.0116 | - |
2,4-dinitrophenyl-ADKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 0.0121 | - |
2,4-dinitrophenyl-ATKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 0.0133 | - |
2,4-dinitrophenyl-ATKLSTSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 0.0152 | - |
2,4-dinitrophenyl-ARKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 0.0155 | - |
2,4-dinitrophenyl-DADKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 0.0177 | - |
2,4-dinitrophenyl-ATKRSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 0.0249 | - |
2,4-dinitrophenyl-ATKDSW | pH 9.5, 37°C | Grifola frondosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.20 | Co2+ | can substitute for Zn2+, enhances activity | Pleurotus ostreatus | |
3.4.24.20 | Zn2+ | zinc-metalloendopetidase | Grifola frondosa | |
3.4.24.20 | Zn2+ | zinc-metalloendopetidase | Pleurotus ostreatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.20 | Grifola frondosa | P81054 | edible mushroom | - |
3.4.24.20 | Pleurotus ostreatus | P81055 | edible mushroom | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.20 | - |
Grifola frondosa |
3.4.24.20 | - |
Pleurotus ostreatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.20 | 2,4-dinitrophenyl-ADKLSW + H2O | - |
Grifola frondosa | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-APKLSW + H2O | - |
Grifola frondosa | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-APKLSW + H2O | - |
Grifola frondosa | 2,4-dinitrophenyl-Ala-Pro + KLSW | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-ARKLSW + H2O | - |
Grifola frondosa | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-ARKLSW + H2O | - |
Grifola frondosa | 2,4-dinitrophenyl-Ala-Arg + KLSW | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-ATKDSW + H2O | - |
Grifola frondosa | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-ATKDSW + H2O | - |
Grifola frondosa | 2,4-dinitrophenyl-Ala-Thr + KDSW | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-ATKLSTSW + H2O | - |
Grifola frondosa | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-ATKLSTSW + H2O | - |
Grifola frondosa | 2,4-dinitrophenyl-Ala-Thr + KLSTSW | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-ATKLSW + H2O | - |
Grifola frondosa | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-ATKLSW + H2O | - |
Grifola frondosa | 2,4-dinitrophenyl-Ala-Thr + KLSW | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-ATKRSW + H2O | - |
Grifola frondosa | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-ATKRSW + H2O | - |
Grifola frondosa | 2,4-dinitrophenyl-Ala-Thr + KRSW | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-DADKLSW + H2O | - |
Grifola frondosa | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-DADKLSW + H2O | - |
Grifola frondosa | 2,4-dinitrophenyl-DAD + KLSW | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-DATKLSW + H2O | - |
Grifola frondosa | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-DATKLSW + H2O | - |
Grifola frondosa | 2,4-dinitrophenyl-DAT + KLSW | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-Ser-Thr-Ala-Thr-Lys-Leu-Ser-Trp + H2O | fluorescent peptide substrate | Grifola frondosa | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-Ser-Thr-Ala-Thr-Lys-Leu-Ser-Trp + H2O | fluorescent peptide substrate | Pleurotus ostreatus | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-STATKLSW + H2O | - |
Grifola frondosa | ? | - |
? | |
3.4.24.20 | 2,4-dinitrophenyl-STATKLSW + H2O | - |
Grifola frondosa | 2,4-dinitrophenyl-STAT + KLSW | - |
? | |
3.4.24.20 | azocasein + H2O | - |
Grifola frondosa | ? | - |
? | |
3.4.24.20 | azocasein + H2O | - |
Pleurotus ostreatus | ? | - |
? | |
3.4.24.20 | additional information | peptides with aspartic acid adjacent to the Lys residue are poor substrates, 2,4-dinitrophenyl-Thr-Lys-Trp and peptides with substitution of L-Arg, L-ornithine, or D-Lys are no substrates | Grifola frondosa | ? | - |
? | |
3.4.24.20 | additional information | peptides with aspartic acid adjacent to the Lys residue are poor substrates, 2,4-dinitrophenyl-Thr-Lys-Trp and peptides with substitution of L-Arg, L-ornithine, or D-Lys are no substrates | Pleurotus ostreatus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.20 | GFMEP | - |
Grifola frondosa |
3.4.24.20 | POMEP | - |
Pleurotus ostreatus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.20 | 0.12 | - |
2,4-dinitrophenyl-DADKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 0.25 | - |
2,4-dinitrophenyl-ATKDSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 0.99 | - |
2,4-dinitrophenyl-ATKLSTSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 1.3 | - |
2,4-dinitrophenyl-ADKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 3 | - |
2,4-dinitrophenyl-ATKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 3.7 | - |
2,4-dinitrophenyl-ATKRSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 5.1 | - |
2,4-dinitrophenyl-DATKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 5.5 | - |
2,4-dinitrophenyl-APKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 17.6 | - |
2,4-dinitrophenyl-ATKLSTSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 21 | - |
2,4-dinitrophenyl-STATKLSW | pH 9.5, 37°C | Grifola frondosa | |
3.4.24.20 | 54.7 | - |
2,4-dinitrophenyl-ARKLSW | pH 9.5, 37°C | Grifola frondosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.20 | 5.6 | - |
azocasein hydrolysis | Pleurotus ostreatus |
3.4.24.20 | 8.5 | - |
rather active with fluorogenic peptide substrates than at pH 5.0 | Pleurotus ostreatus |
3.4.24.20 | 9.5 | - |
- |
Grifola frondosa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.20 | 6 | 10.5 | exhibits more than 50% of the maximal activity over this range | Grifola frondosa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.20 | 0.0399 | - |
2,4-dinitrophenyl-Ala-Thr-D-lysine-Leu-Ser-Try | pH 9.5, 37°C | Grifola frondosa |