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Literature summary extracted from

  • Bhardwaj, R.; Singh, B.; Bhat, T.K.
    Purification and characterization of tannin acyl hydrolase from Aspergillus niger MTCC 2425 (2003), J. Basic Microbiol., 43, 449-461.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 additional information not affected by EDTA Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 Ca2+ 58% inhibition at 20 mM, noncompetitive Aspergillus niger
3.1.1.20 Cd2+ 55% inhibition at 20 mM, noncompetitive Aspergillus niger
3.1.1.20 Mg2+ 33% inhibition at 20 mM, noncompetitive Aspergillus niger
3.1.1.20 Mn2+ 54% inhibition at 20 mM, noncompetitive Aspergillus niger
3.1.1.20 additional information not affected by EDTA Aspergillus niger
3.1.1.20 Zn2+ 41% inhibition at 20 mM, noncompetitive Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 0.28
-
 Tannic acid
-
 Aspergillus niger
3.1.1.20 0.95
-
 methyl gallate
-
 Aspergillus niger
3.1.1.20 2.05
-
 propyl gallate
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 83000
-
 x * 102000, SDS-PAGE, x * 83000, SDS-PAGE Aspergillus niger
3.1.1.20 102000
-
 x * 102000, SDS-PAGE, x * 83000, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus niger
-
-
-
3.1.1.20 Aspergillus niger MTCC 2425
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 best at pH 5.5, overview, 51fold Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.20 mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 355.6
-
 purified enzyme Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 methyl gallate + H2O
-
 Aspergillus niger gallate + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Aspergillus niger MTCC 2425 gallate + methanol
-
 ?
3.1.1.20 propyl gallate + H2O
-
 Aspergillus niger gallate + propanol
-
 ?
3.1.1.20 propyl gallate + H2O
-
 Aspergillus niger MTCC 2425 gallate + propanol
-
 ?
3.1.1.20 tannic acid + H2O best substrate Aspergillus niger 10 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O best substrate Aspergillus niger MTCC 2425 10 gallate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 ? x * 102000, SDS-PAGE, x * 83000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase
-
 Aspergillus niger

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.20 0.59
-
 Ca2+
-
 Aspergillus niger