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Literature summary extracted from

  • Banerjee, D.; Mondal, K.C.; Pati, B.R.
    Production and characterization of extracellular and intracellular tannase from newly isolated Aspergillus aculeatus DBF 9 (2001), J. Basic Microbiol., 41, 313-318.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 Tannic acid required for enzyme induction, best at 1% w/v tannic acid for the intracellular enzyme, and at 2% w/v for the extracellular enzyme Aspergillus aculeatus

Application

EC Number Application Comment Organism
3.1.1.20 nutrition the enzyme has wide applications in food, beverage, brewing, cosmetic, and chemical industry, overview Aspergillus aculeatus
3.1.1.20 synthesis the enzyme has wide applications in food, beverage, brewing, cosmetic, and chemical industry, overview Aspergillus aculeatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 D-glucose slightly stimulating, inhibits intracellular enzyme production at concentrations above 0.05% w/v, and extracellular enzyme production above 0.1% w/v Aspergillus aculeatus
3.1.1.20 additional information no inhibition of the extracellular enzyme by PMSF Aspergillus aculeatus
3.1.1.20 NaCl inhibits above 3 M Aspergillus aculeatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.20 extracellular 5fold lower production level in culture than the intracellular form Aspergillus aculeatus
-
-
3.1.1.20 intracellular 5fold higher production level in culture than the extracellular form Aspergillus aculeatus 5622
-
3.1.1.20 membrane intracellular enzyme Aspergillus aculeatus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 digallate + H2O Aspergillus aculeatus substrate: tannins gallate i.e. 3,4,5-trihydroxybenzoate ?
3.1.1.20 digallate + H2O Aspergillus aculeatus DBF 9 substrate: tannins gallate i.e. 3,4,5-trihydroxybenzoate ?
3.1.1.20 tannic acid + H2O Aspergillus aculeatus enzyme hydrolyzes the ester and depside linkages of tannic acid 10 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O Aspergillus aculeatus DBF 9 enzyme hydrolyzes the ester and depside linkages of tannic acid 10 gallate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus aculeatus
-
 intra-and extracellular enzyme forms, inducible, fungus can utilize tannic acid as sole carbon and energy source
-
3.1.1.20 Aspergillus aculeatus DBF 9
-
 intra-and extracellular enzyme forms, inducible, fungus can utilize tannic acid as sole carbon and energy source
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 digallate + H2O substrate: tannins Aspergillus aculeatus gallate
-
 ?
3.1.1.20 digallate + H2O substrate: tannins Aspergillus aculeatus gallate i.e. 3,4,5-trihydroxybenzoate ?
3.1.1.20 digallate + H2O substrate: tannins Aspergillus aculeatus DBF 9 gallate
-
 ?
3.1.1.20 digallate + H2O substrate: tannins Aspergillus aculeatus DBF 9 gallate i.e. 3,4,5-trihydroxybenzoate ?
3.1.1.20 tannic acid + H2O enzyme hydrolyzes the ester and depside linkages of tannic acid Aspergillus aculeatus 10 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O enzyme hydrolyzes the ester and depside linkages of tannic acid Aspergillus aculeatus DBF 9 10 gallate + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase
-
 Aspergillus aculeatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 50
-
 extracellular enzyme form Aspergillus aculeatus
3.1.1.20 60
-
 intracellular enzyme form Aspergillus aculeatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 50
-
 stable up to, extracellular enzyme form Aspergillus aculeatus
3.1.1.20 60
-
 stable up to, intracellular enzyme form Aspergillus aculeatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5
-
 intra- and extracellular enzyme form Aspergillus aculeatus