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Literature summary extracted from

  • Veiga-Malta, I.; Duarte, M.; Dinis, M.; Madureira, P.; Ferreira, P.; Videira, A.
    Identification of NAD+ synthetase from Streptococcus sobrinus as a B-cell-stimulatory protein (2004), J. Bacteriol., 186, 419-426.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.5 functional expression in Escherichia coli strain M-15 as His-tagged enzyme, secretion to the medium Streptococcus sobrinus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.5 extracellular secretion Streptococcus sobrinus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.5 Mg2+ required Streptococcus sobrinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.5 38000
-
 x * 38000, recombinant His-tagged enzyme, SDS-PAGE Streptococcus sobrinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3 Streptococcus sobrinus
-
 AMP + diphosphate + NAD+
-
 ?
6.3.1.5 ATP + deamido-NAD+ + NH3 Streptococcus sobrinus 6715
-
 AMP + diphosphate + NAD+
-
 ?
6.3.1.5 additional information Streptococcus sobrinus enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures ?
-
 ?
6.3.1.5 additional information Streptococcus sobrinus 6715 enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.5 Streptococcus sobrinus
-
-
-
6.3.1.5 Streptococcus sobrinus 6715
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.5 recombinant as His-tagged enzyme from Escherichia coli Streptococcus sobrinus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.5 0.49
-
 purified recombinant His-tagged enzyme Streptococcus sobrinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3
-
 Streptococcus sobrinus AMP + diphosphate + NAD+
-
 ?
6.3.1.5 ATP + deamido-NAD+ + NH3
-
 Streptococcus sobrinus 6715 AMP + diphosphate + NAD+
-
 ?
6.3.1.5 additional information enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures Streptococcus sobrinus ?
-
 ?
6.3.1.5 additional information enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures Streptococcus sobrinus 6715 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.5 ? x * 38000, recombinant His-tagged enzyme, SDS-PAGE Streptococcus sobrinus

Synonyms

EC Number Synonyms Comment Organism
6.3.1.5 NAD synthase
-
 Streptococcus sobrinus
6.3.1.5 NAD synthetase
-
 Streptococcus sobrinus
6.3.1.5 Nicotinamide adenine dinucleotide synthetase
-
 Streptococcus sobrinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.5 37
-
-
 Streptococcus sobrinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.1.5 95
-
 10 min, inactivation Streptococcus sobrinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.5 8.5
-
 assay at Streptococcus sobrinus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.5 ATP required Streptococcus sobrinus