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Literature summary extracted from

  • Marx, C.J.; Laukel, M.; Vorholt, J.A.; Lidstrom, M.E.
    Purification of the formate-tetrahydrofolate ligase from Methylobacterium extorquens AM1 and demonstration of its requirement for methylotrophic growth (2003), J. Bacteriol., 185, 7169-7175.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.3 0.021
-
 ATP 30°C, pH 8.0 Methylorubrum extorquens
6.3.4.3 0.8
-
 tetrahydrofolate 30°C, pH 8.0 Methylorubrum extorquens
6.3.4.3 22
-
 formate 30°C, pH 8.0 Methylorubrum extorquens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.3 60000
-
 4 * 60000, SDS-PAGE Methylorubrum extorquens
6.3.4.3 240000
-
 gel filtration Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate Methylorubrum extorquens FtfL activity is required for methylotrophic growth ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 FtfL activity is required for methylotrophic growth ADP + phosphate + 10-formyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.3 Methylorubrum extorquens Q83WS0
-
-
6.3.4.3 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 Q83WS0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.3 ammonium sulfate, Phenyl Sepharose, Source15Q, hydroxyapatite, Resource Q Methylorubrum extorquens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.3 117
-
-
 Methylorubrum extorquens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Methylorubrum extorquens ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate FtfL activity is required for methylotrophic growth Methylorubrum extorquens ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate FtfL activity is required for methylotrophic growth Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 ADP + phosphate + 10-formyltetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.3 tetramer 4 * 60000, SDS-PAGE Methylorubrum extorquens

Synonyms

EC Number Synonyms Comment Organism
6.3.4.3 ftfL
-
 Methylorubrum extorquens