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Literature summary extracted from

  • Grimek, T.L.; Holden, H.; Rayment, I.; Escalante-Semerena, J.C.
    Residues C123 and D58 of the 2-methylisocitrate lyase (PrpB) enzyme of Salmonella enterica are essential for catalysis (2003), J. Bacteriol., 185, 4837-4843.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.30 dithiothreitol 5 mM, required for optimal activity Salmonella enterica
4.1.3.30 glutathione required for optimal activity Salmonella enterica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.30
-
 Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.30 C123A no enzyme activity Salmonella enterica
4.1.3.30 D58A no enzyme activity Salmonella enterica
4.1.3.30 H125A decreased kcat Salmonella enterica
4.1.3.30 K121A decreased kcat Salmonella enterica
4.1.3.30 R122K decreased kcat Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.30 10
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, H125A mutant Salmonella enterica
4.1.3.30 19
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, wild-type Salmonella enterica
4.1.3.30 22
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, R122K mutant Salmonella enterica
4.1.3.30 57
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, K121A mutant Salmonella enterica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.30 Mg2+ requirement Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.30 32000
-
 4 * 32000, SDS-PAGE Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.30 additional information Salmonella enterica enzyme in methylcitrate cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.30 Salmonella enterica
-
 serovar typhimurium LT2
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.3.30 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = succinate + pyruvate mechanism Salmonella enterica
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.30 (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate syn: methylisocitrate Salmonella enterica Pyruvate + succinate
-
 ?
4.1.3.30 additional information no substrate: isocitrate Salmonella enterica ?
-
 ?
4.1.3.30 additional information enzyme in methylcitrate cycle Salmonella enterica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.30 tetramer 4 * 32000, SDS-PAGE Salmonella enterica

Synonyms

EC Number Synonyms Comment Organism
4.1.3.30 PrpB
-
 Salmonella enterica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.30 50
-
-
 Salmonella enterica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.30 50
-
 14% activity after 10 min Salmonella enterica
4.1.3.30 60
-
 no detectable activity Salmonella enterica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.30 0.1
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, K121A mutant Salmonella enterica
4.1.3.30 0.14
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, H125A mutant Salmonella enterica
4.1.3.30 38
-
 Mg2+ pH 7.5, 37°C Salmonella enterica
4.1.3.30 55
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, R122K mutant Salmonella enterica
4.1.3.30 105
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, wild-type Salmonella enterica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.30 7.5 8
-
 Salmonella enterica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.30 6 8.5 16% activity at pH 6, 96% activity at pH 8.5 Salmonella enterica