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Literature summary extracted from

  • Mulrooney, S.B.; Hausinger, R.P.
    Metal ion dependence of recombinant Escherichia coli allantoinase (2003), J. Bacteriol., 185, 126-134.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.5 overproduction of enzyme in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.5 dithiothreitol competitive Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.5 17
-
 (S)-allantoin pH 7.4, 37°C, Zn2+-amended culture Escherichia coli
3.5.2.5 19.5
-
 (S)-allantoin pH 7.4, 37°C, Co2+-amended culture Escherichia coli
3.5.2.5 79.9
-
 (S)-allantoin pH 7.4, 37°C, Ni2+-amended culture Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.5 recombinant enzyme Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.5 76.1
-
 pH 7.4, 37°C, Zn2+-amended culture Escherichia coli
3.5.2.5 410
-
 pH 7.4, 37°C, Co2+-amended culture Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.5 (S)-allantoin + H2O zinc enzyme uses only the S-isomer, cobalt-enzyme prefers S-isomer but also hydrolyses R-isomer Escherichia coli allantoate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.2.5 0.237
-
 dithiothreitol pH 7.4, 37°C, Co2+-amended culture Escherichia coli
3.5.2.5 0.795
-
 dithiothreitol pH 7.4, 37°C, Zn2+-amended culture Escherichia coli