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Literature summary extracted from

  • Herter, S.; Busch, A.; Fuchs, G.
    L-Malyl-coenzyme A lyase/beta-methylmalyl-coenzyme A lyase from Chloroflexus aurantiacus, a bifunctional enzyme involved in autotrophic CO(2) fixation (2002), J. Bacteriol., 184, 5999-6006.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.24 expressed in Escherichia coli Chloroflexus aurantiacus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.24 EDTA inhibits at 1 mM Chloroflexus aurantiacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.24 0.061
-
 (3S)-3-carboxy-3-hydroxypropanoyl-CoA pH 7.1, 55°C Chloroflexus aurantiacus
4.1.3.24 0.089
-
 beta-methylmalyl-CoA pH 7.1, 55°C Chloroflexus aurantiacus
4.1.3.24 0.36
-
 acetyl-CoA pH 7.1, 55°C Chloroflexus aurantiacus
4.1.3.24 1.2
-
 propionyl-CoA pH 7.1, 55°C Chloroflexus aurantiacus
4.1.3.24 2
-
 glyoxylate pH 7.1, 55°C Chloroflexus aurantiacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.24 Mg2+ 4 mM, requirement Chloroflexus aurantiacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.24 38000
-
 x * 38000, alpha5 or alpha6, SDS-PAGE Chloroflexus aurantiacus
4.1.3.24 210000
-
 gel filtration Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.24 (3S)-3-carboxy-3-hydroxypropanoyl-CoA Chloroflexus aurantiacus
-
 acetyl-CoA + glyoxylate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.24 Chloroflexus aurantiacus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.24
-
 Chloroflexus aurantiacus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.24 0.48
-
 acetyl-CoA condensation Chloroflexus aurantiacus
4.1.3.24 0.5
-
 propionyl-CoA condensation Chloroflexus aurantiacus
4.1.3.24 2.3
-
 L-malyl-CoA lyase activity Chloroflexus aurantiacus
4.1.3.24 6.5
-
 beta-methylmalyl-CoA lyase activity Chloroflexus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.24 (3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
 Chloroflexus aurantiacus acetyl-CoA + glyoxylate
-
 r
4.1.3.24 (3S)-3-carboxy-3-hydroxypropanoyl-CoA malyl-CoA Chloroflexus aurantiacus acetyl-CoA + glyoxylate
-
 r
4.1.3.24 propionyl-CoA + glyoxylate
-
 Chloroflexus aurantiacus erythro-beta-methylmalyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.24 ? x * 38000, alpha5 or alpha6, SDS-PAGE Chloroflexus aurantiacus

Synonyms

EC Number Synonyms Comment Organism
4.1.3.24 L-malyl-CoA lyase/beta-methylmalyl-CoA lyase the two enzymes are identical and both enzymatic reactions are catalysed by one single bifunctional enzyme Chloroflexus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.24 70
-
-
 Chloroflexus aurantiacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.24 65
-
 stable for 20 min Chloroflexus aurantiacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.24 8.8
-
 (3S)-3-carboxy-3-hydroxypropanoyl-CoA pH 7.1, 55°C Chloroflexus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.24 7.1
-
 at 55°C Chloroflexus aurantiacus