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Literature summary extracted from

  • Smith, K.S.; Ingram-Smith, C.; Ferry, J.G.
    Roles of the conserved aspartate and arginine in the catalytic mechanism of an archaeal beta-class carbonic anhydrase (2002), J. Bacteriol., 184, 4240-4245.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 wild-type and variant carbonic anhydrases heterologously produced in Escherichia coli BL21(DE3) Methanothermobacter thermautotrophicus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.1 D34A site-directed mutagenesis Methanothermobacter thermautotrophicus
4.2.1.1 D34A/R36A site-directed mutagenesis Methanothermobacter thermautotrophicus
4.2.1.1 D34E site-directed mutagenesis Methanothermobacter thermautotrophicus
4.2.1.1 R36A site-directed mutagenesis Methanothermobacter thermautotrophicus
4.2.1.1 R36K site-directed mutagenesis Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 1.8
-
 CO2 pH 8.5, 25°C, mutant D34E Methanothermobacter thermautotrophicus
4.2.1.1 2.7
-
 CO2 pH 8.5, 25°C, wild-type, MOPS buffer Methanothermobacter thermautotrophicus
4.2.1.1 2.9
-
 CO2 pH 8.5, 25°C, wild-type Methanothermobacter thermautotrophicus
4.2.1.1 3.5
-
 CO2 pH 8.5, 25°C, mutant D34A, MOPS buffer Methanothermobacter thermautotrophicus
4.2.1.1 3.6
-
 CO2 pH 8.5, 25°C, mutant D34A Methanothermobacter thermautotrophicus
4.2.1.1 4.2
-
 CO2 pH 8.5, 25°C, wild-type, imidazole buffer Methanothermobacter thermautotrophicus
4.2.1.1 5.8
-
 CO2 pH 8.5, 25°C, mutant D34A/R36A, imidazole buffer Methanothermobacter thermautotrophicus
4.2.1.1 7.2
-
 CO2 pH 8.5, 25°C, mutant R36K Methanothermobacter thermautotrophicus
4.2.1.1 9.2
-
 CO2 pH 8.5, 25°C, mutant D34A/R36A, MOPS buffer Methanothermobacter thermautotrophicus
4.2.1.1 15.3
-
 CO2 pH 8.5, 25°C, mutant D34A, imidazole buffer Methanothermobacter thermautotrophicus
4.2.1.1 35.3
-
 CO2 pH 8.5, 25°C, mutant R36A, MOPS buffer Methanothermobacter thermautotrophicus
4.2.1.1 36.2
-
 CO2 pH 8.5, 25°C, mutant R36A Methanothermobacter thermautotrophicus
4.2.1.1 47.1
-
 CO2 pH 8.5, 25°C, mutant R36A, imidazole buffer Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ zinc-hydroxide mechanism of catalysis Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Methanothermobacter thermautotrophicus
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1
-
 Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Methanothermobacter thermautotrophicus CO2 + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 beta-class carbonic anhydrase
-
 Methanothermobacter thermautotrophicus
4.2.1.1 Cab
-
 Methanothermobacter thermautotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 24
-
 CO2 pH 8.5, 25°C, mutant R36A, MOPS buffer Methanothermobacter thermautotrophicus
4.2.1.1 30
-
 CO2 pH 8.5, 25°C, mutant R36A, imidazole buffer Methanothermobacter thermautotrophicus
4.2.1.1 46
-
 CO2 pH 8.5, 25°C, mutant R36A Methanothermobacter thermautotrophicus
4.2.1.1 92
-
 CO2 pH 8.5, 25°C, mutant R36K Methanothermobacter thermautotrophicus
4.2.1.1 260
-
 CO2 pH 8.5, 25°C, mutant D34A/R36A, MOPS buffer Methanothermobacter thermautotrophicus
4.2.1.1 520
-
 CO2 pH 8.5, 25°C, mutant D34A/R36A Methanothermobacter thermautotrophicus
4.2.1.1 1100
-
 CO2 pH 8.5, 25°C, mutant D34A, MOPS buffer Methanothermobacter thermautotrophicus
4.2.1.1 1500
-
 CO2 pH 8.5, 25°C, mutant D34A/R36A, imidazole buffer Methanothermobacter thermautotrophicus
4.2.1.1 3600
-
 CO2 pH 8.5, 25°C, mutant D34E Methanothermobacter thermautotrophicus
4.2.1.1 4200
-
 CO2 pH 8.5, 25°C, mutant D34A Methanothermobacter thermautotrophicus
4.2.1.1 9100
-
 CO2 pH 8.5, 25°C, mutant D34A, imidazole buffer Methanothermobacter thermautotrophicus
4.2.1.1 11000
-
 CO2 pH 8.5, 25°C, wild-type, MOPS buffer Methanothermobacter thermautotrophicus
4.2.1.1 13000
-
 CO2 pH 8.5, 25°C, wild-type, imidazole buffer Methanothermobacter thermautotrophicus
4.2.1.1 24000
-
 CO2 pH 8.5, 25°C, wild-type Methanothermobacter thermautotrophicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.1 6.4 9
-
 Methanothermobacter thermautotrophicus