EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.208 | boxA gene | Aromatoleum evansii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.208 | Fe | the reductase componment BoxA contains 10.4 to 18.4 mol of Fe, and 13.3 to 17.9 mol of acid-labile sulfur per mol of native protein, depending on the method of protein determination | Aromatoleum evansii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.208 | 45883 | - |
2 * 45883, component BoxA, calculated from sequence | Aromatoleum evansii |
1.14.13.208 | 50000 | - |
2 * 50000, component BoxA, SDS-PAGE | Aromatoleum evansii |
1.14.13.208 | 98000 | - |
BoxA, gel filtration | Aromatoleum evansii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.208 | benzoyl-CoA + NADPH + H+ + O2 | Aromatoleum evansii | second step in aerobic benzoate metabolism, enzyme is induced by benzoate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.208 | Aromatoleum evansii | - |
- |
- |
6.2.1.25 | Aromatoleum evansii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.208 | BoxA | Aromatoleum evansii |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.208 | culture condition:benzoate-grown cell | - |
Aromatoleum evansii | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.208 | benzoyl-CoA + NADPH + H+ + O2 | second step in aerobic benzoate metabolism, enzyme is induced by benzoate | Aromatoleum evansii | ? | - |
? | |
1.14.13.208 | additional information | purified reductase component BoxA catalyses, in the absence of BoxB, the electron transfer from NADPH to free FAD with a rate of 17 mmol/min*mg protein. This activity is very high compared to the benzoyl-CoA conversion rate with purified BoxAB (0.29 mmol/min*mg BoxA). The reductase component BoxA oxidizes NADPH to transfer electrons, mediated by enzyme-bound FAD, to one or two of the [4Fe-4S] centres of the protein. The flow of electrons from NADPH to the artificial electron acceptor, free FAD, is greatly (10-fold) stimulated by binding of benzoyl-CoA to BoxA | Aromatoleum evansii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.208 | ? | 2 * 45883, component BoxA, calculated from sequence | Aromatoleum evansii |
1.14.13.208 | ? | 2 * 50000, component BoxA, SDS-PAGE | Aromatoleum evansii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.208 | benzoyl-CoA oxygenase | - |
Aromatoleum evansii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.208 | FAD | the reductase componment BoxA contains 0.72 mol flavin adenine dinucleotide. BoxA oxidizes NADPH to transfer electrons, mediated by enzyme-bound FAD, to one or two of the [4Fe-4S] centres of the protein. The flow of electrons from NADPH to the artificial electron acceptor, free FAD, is greatly (10-fold) stimulated by binding of benzoyl-CoA to BoxA | Aromatoleum evansii | |
1.14.13.208 | NADPH | the reductase component BoxA oxidizes NADPH to transfer electrons, mediated by enzyme-bound FAD, to one or two of the [4Fe-4S] centres of the protein. The flow of electrons from NADPH to the artificial electron acceptor, free FAD, is greatly (10-fold) stimulated by binding of benzoyl-CoA to BoxA. No oxidation of NADH | Aromatoleum evansii |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.14.13.208 | Aromatoleum evansii | component BoxA, calculated from sequence | - |
5.6 |