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Literature summary extracted from

  • Smith, K.S.; Cosper, N.J.; Stalhandske, C.; Scott, R.A.; Ferry, J.G.
    Structural and kinetic characterization of an archaeal beta-class carbonic anhydrase (2000), J. Bacteriol., 182, 6605-6613.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 heterologously produced in Escherichia coli Methanothermobacter thermautotrophicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.1
-
 Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ zinc hydroxide mechanism Methanothermobacter thermautotrophicus
4.2.1.1 Zn2+ zinc hydroxide mechanism Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Methanothermobacter thermautotrophicus
-
 CO2 + H2O
-
 ?
4.2.1.1 H2CO3 Spinacia oleracea
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Methanothermobacter thermautotrophicus Q50565
-
-
4.2.1.1 Spinacia oleracea P16016 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 recombinant enzyme Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Methanothermobacter thermautotrophicus CO2 + H2O
-
 ?
4.2.1.1 H2CO3
-
 Spinacia oleracea CO2 + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.1 octamer
-
 Spinacia oleracea
4.2.1.1 tetramer
-
 Methanothermobacter thermautotrophicus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 beta-class carbonic anhydrase
-
 Methanothermobacter thermautotrophicus
4.2.1.1 beta-class carbonic anhydrase
-
 Spinacia oleracea
4.2.1.1 Cab
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1 65 75
-
 Spinacia oleracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.1 85
-
 most thermostable carbonic anhydrase, retains 90% activity after incubation for 15 min Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.1 5.5 7.5
-
 Methanothermobacter thermautotrophicus