BRENDA - Enzyme Database

Characterization of activity and expression of isocitrate lyase in Mycobacterium avium and Mycobacterium tuberculosis

Honer Zu Bentrup, K.; Miczak, A.; Swenson, D.L.; Russell, D.G.; J. Bacteriol. 181, 7161-7167 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
Icl and AceA are different open reading frames, both with isocitrate lyase activity
Mycobacterium tuberculosis
General Stability
EC Number
General Stability
Organism
4.1.3.1
unstable, loses its activity within a couple of hours on ice, AceA enzyme
Mycobacterium tuberculosis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
3-nitropropionate
-
Mycobacterium tuberculosis
4.1.3.1
Bromopyruvate
-
Mycobacterium tuberculosis
4.1.3.1
Itaconate
-
Mycobacterium tuberculosis
4.1.3.1
itaconic anhydride
-
Mycobacterium tuberculosis
4.1.3.1
malate
Icl enzyme, 50% inhibition at 5 mM
Mycobacterium tuberculosis
4.1.3.1
oxalate
Icl enzyme, 50% inhibition at 5 mM
Mycobacterium tuberculosis
4.1.3.1
succinate
AceA enzyme, 50% inhibition at 5 mM
Mycobacterium tuberculosis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.145
-
threo D-(s)-isocitrate
Icl enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
1.3
-
threo D-(s)-isocitrate
AceA enzyme, Tricine buffer, 37C, pH 7.5
Mycobacterium tuberculosis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
Icl enzyme: most effective cation, optimal concentration: 5 mM
Mycobacterium tuberculosis
4.1.3.1
Mn2+
Icl enzyme: can replace Mg2+, yielding 39% of the activity obtained with Mg2+
Mycobacterium tuberculosis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
50000
-
calculated from amino acid sequence
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Mycobacterium tuberculosis
-
succinate + glyoxylate
-
Mycobacterium tuberculosis
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Mycobacterium tuberculosis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Mycobacterium tuberculosis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.3.1
1.3
-
Icl enzyme
Mycobacterium tuberculosis
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
-80C, stable for several months when frozen in liquid nitrogen and stored at -80C, Icl enzyme
Mycobacterium tuberculosis
4.1.3.1
4C, after thawing and storage at 4C the loss of activity after 2 months is minimal in the presence of glycerol and a reducing agent, Icl enzyme
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
651648
Mycobacterium tuberculosis
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
651648
Mycobacterium tuberculosis
succinate + glyoxylate
-
651648
Mycobacterium tuberculosis
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
-
Icl enzyme
Mycobacterium tuberculosis
4.1.3.1
7.5
-
AceA enzyme
Mycobacterium tuberculosis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.3.1
0.003
-
3-nitropropionate
Icl enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.11
-
3-nitropropionate
AceA enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.12
-
Bromopyruvate
Icl enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.12
-
Itaconate
Icl enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.19
-
itaconic anhydride
Icl enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.22
-
Itaconate
AceA enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.48
-
itaconic anhydride
AceA enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.71
-
Bromopyruvate
AceA enzyme, 37C
Mycobacterium tuberculosis
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.1.3.1
Mycobacterium tuberculosis
calculated from amino acid sequence
-
5
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
Icl and AceA are different open reading frames, both with isocitrate lyase activity
Mycobacterium tuberculosis
General Stability (protein specific)
EC Number
General Stability
Organism
4.1.3.1
unstable, loses its activity within a couple of hours on ice, AceA enzyme
Mycobacterium tuberculosis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
3-nitropropionate
-
Mycobacterium tuberculosis
4.1.3.1
Bromopyruvate
-
Mycobacterium tuberculosis
4.1.3.1
Itaconate
-
Mycobacterium tuberculosis
4.1.3.1
itaconic anhydride
-
Mycobacterium tuberculosis
4.1.3.1
malate
Icl enzyme, 50% inhibition at 5 mM
Mycobacterium tuberculosis
4.1.3.1
oxalate
Icl enzyme, 50% inhibition at 5 mM
Mycobacterium tuberculosis
4.1.3.1
succinate
AceA enzyme, 50% inhibition at 5 mM
Mycobacterium tuberculosis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.3.1
0.003
-
3-nitropropionate
Icl enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.11
-
3-nitropropionate
AceA enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.12
-
Bromopyruvate
Icl enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.12
-
Itaconate
Icl enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.19
-
itaconic anhydride
Icl enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.22
-
Itaconate
AceA enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.48
-
itaconic anhydride
AceA enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
0.71
-
Bromopyruvate
AceA enzyme, 37C
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.145
-
threo D-(s)-isocitrate
Icl enzyme, 37C
Mycobacterium tuberculosis
4.1.3.1
1.3
-
threo D-(s)-isocitrate
AceA enzyme, Tricine buffer, 37C, pH 7.5
Mycobacterium tuberculosis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
Icl enzyme: most effective cation, optimal concentration: 5 mM
Mycobacterium tuberculosis
4.1.3.1
Mn2+
Icl enzyme: can replace Mg2+, yielding 39% of the activity obtained with Mg2+
Mycobacterium tuberculosis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
50000
-
calculated from amino acid sequence
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Mycobacterium tuberculosis
-
succinate + glyoxylate
-
Mycobacterium tuberculosis
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Mycobacterium tuberculosis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.3.1
1.3
-
Icl enzyme
Mycobacterium tuberculosis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
-80C, stable for several months when frozen in liquid nitrogen and stored at -80C, Icl enzyme
Mycobacterium tuberculosis
4.1.3.1
4C, after thawing and storage at 4C the loss of activity after 2 months is minimal in the presence of glycerol and a reducing agent, Icl enzyme
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
651648
Mycobacterium tuberculosis
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
651648
Mycobacterium tuberculosis
succinate + glyoxylate
-
651648
Mycobacterium tuberculosis
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
-
Icl enzyme
Mycobacterium tuberculosis
4.1.3.1
7.5
-
AceA enzyme
Mycobacterium tuberculosis
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.1.3.1
Mycobacterium tuberculosis
calculated from amino acid sequence
-
5