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Literature summary extracted from

  • Smith, K.S.; Ferry, J.G.
    A plant-type (beta-class) carbonic anhydrase in the thermophilic methanoarchaeon Methanobacterium thermoautotrophicum (1999), J. Bacteriol., 181, 6247-6253.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 DELTAH cab gene hyperexpressed in Escherichia coli Methanothermobacter thermautotrophicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 azide
-
 Methanothermobacter thermautotrophicus
4.2.1.1 Iodide
-
 Methanothermobacter thermautotrophicus
4.2.1.1 nitrate
-
 Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 2.9
-
 CO2 pH 8.5, 25°C Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ zinc enzyme Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 18900
-
 4 * 21000, SDS-PAGE, 4 * 18900, calculated on the basis of the amino acid composition deduced from the nucleotide sequence Methanothermobacter thermautotrophicus
4.2.1.1 21000
-
 4 * 21000, SDS-PAGE, 4 * 18900, calculated on the basis of the amino acid composition deduced from the nucleotide sequence Methanothermobacter thermautotrophicus
4.2.1.1 90000
-
 gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Methanothermobacter thermautotrophicus
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 recombinant protein Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.1 additional information
-
 specific activity 39.0 U/mg, one unit = (t0-t)/t, where t0 is the time for the uncatalyzed reaction and t is the time of the catalyzed reaction Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Methanothermobacter thermautotrophicus CO2 + H2O
-
 r
4.2.1.1 additional information no esterase activity detected with p-phenylacetate as the substrate Methanothermobacter thermautotrophicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.1 tetramer 4 * 21000, SDS-PAGE, 4 * 18900, calculated on the basis of the amino acid composition deduced from the nucleotide sequence Methanothermobacter thermautotrophicus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 plant-type (beta-class) carbonic anhydrase
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1 65
-
-
 Methanothermobacter thermautotrophicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.1 75
-
 thermostable up to 75°C Methanothermobacter thermautotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 17000
-
 CO2 pH 8.5, 25°C Methanothermobacter thermautotrophicus