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Literature summary extracted from

  • Harano, Y.; Suzuki, I.; Maeda, S.; Kaneko, T.; Tabata, S.; Omata, T.
    Identification and nitrogen regulation of the cyanase gene from the cyanobacteria Synechocystis sp. strain PCC 6803 and Synechococcus sp. strain PCC 7942 (1997), J. Bacteriol., 179, 5744-5750.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.104 bicarbonate
-
 Synechococcus sp.
4.2.1.104 bicarbonate
-
 Synechocystis sp.
4.2.1.104 NtcA transcription factor, global nitrogen regulator of cyanobacteria, required for expression Synechococcus sp.
4.2.1.104 NtcA transcription factor, global nitrogen regulator of cyanobacteria, required for expression Synechocystis sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.104 gene cynS, DNA and amino acid sequence determination and analysis, tightly clustered with 2 genes located upstream, which encode proteins similar to the subunits of nitrate-nitrite transporter, complementation of deficient Escherichia coli strain Synechococcus sp.
4.2.1.104 gene cynS, tightly clustered with 4 putative molybdenum cofactor biosynthesis genes located downstream Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.104 NH4+ negative regulation, represses enzyme expression Synechococcus sp.
4.2.1.104 NH4+ negative regulation, represses enzyme expression Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.104 16362
-
 x * 16362, amino acid sequence calculation Synechococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.104 Synechococcus sp.
-
 strain PCC 7942, gene cynS
-
4.2.1.104 Synechocystis sp.
-
 strain PCC 6803, gene cynS
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.104 cyanate + HCO3- + 2 H+ = NH3 + 2 CO2 the enzyme requires bicarbonate as a cofactor. Its mechanism is to catalyse the attack of bicarbonate on cyanate, with elimination of carbon dioxide, thus catalysing hydration of the cyanate to carbamae. The carbamate spontaneously hydrolyses to ammonia and carbon dioxide Synechococcus sp.
a
4.2.1.104 cyanate + HCO3- + 2 H+ = NH3 + 2 CO2 the enzyme requires bicarbonate as a cofactor. Its mechanism is to catalyse the attack of bicarbonate on cyanate, with elimination of carbon dioxide, thus catalysing hydration of the cyanate to carbamate. The carbamate spontaneously hydrolyses to ammonia and carbon dioxide Synechocystis sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.104 0.047
-
 complemented deficient Escherichia coli cells Synechococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.104 NCO- + HCO3-
-
 Synechococcus sp. NH4+ + CO2
-
 ?
4.2.1.104 NCO- + HCO3-
-
 Synechocystis sp. NH4+ + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.104 ? x * 16362, amino acid sequence calculation Synechococcus sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.1.104 cyanase
-
 Synechococcus sp.
4.2.1.104 cyanase
-
 Synechocystis sp.
4.2.1.104 EC 4.3.99.1 formerly Synechococcus sp.
4.2.1.104 EC 4.3.99.1 formerly Synechocystis sp.