6.3.5.9
0.00104
adenosyl-cobyrinic acid a,c-diamide
pH 7.5, 20°C
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.00121
adenosyl-cobyrinic acid tetraamide
pH 7.5, 20°C
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.00122
adenosyl-cobyrinic acid triamide
pH 7.5, 20°C
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.00125
adenosyl-cobyrinic acid pentaamide
pH 7.5, 20°C
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.011
ATP
pH 7.5, 20°C, adenosyl-cobyrinic acid a,c-diamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.033
L-glutamine
pH 7.5, 20°C, adenosyl-cobyrinic acid triamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.035
ATP
pH 7.5, 20°C, adenosyl-cobyrinic acid triamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.045
L-glutamine
pH 7.5, 20°C, adenosyl-cobyrinic acid a,c-diamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.049
L-glutamine
pH 7.5, 20°C, adenosyl-cobyrinic acid pentaamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.064
L-glutamine
pH 7.5, 20°C, adenosyl-cobyrinic acid tetraamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.088
ATP
pH 7.5, 20°C, adenosyl-cobyrinic acid pentaamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.12
ATP
pH 7.5, 20°C, adenosyl-cobyrinic acid tetraamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
20
ammonia
pH 7.5, 20°C
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
additional information
additional information
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.00104
adenosylcobyrinic acid a,c-diamide
pH 7.5
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.00121
adenosylcobyrinic acid tetraamide
pH 7.5
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.00122
adenosylcobyrinic acid triamide
pH 7.5
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.00125
adenosylcobyrinic acid pentaamide
pH 7.5
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.011
ATP
pH 7.5, adenosylcobyrinic acid a,c-diamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.033
L-glutamine
pH 7.5, adenosylcobyrinic acid triamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.035
ATP
pH 7.5, adenosylcobyrinic acid triamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.045
L-glutamine
pH 7.5, adenosylcobyrinic acid a,c-diamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.049
L-glutamine
pH 7.5, adenosylcobyrinic acid pentaamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.064
L-glutamine
pH 7.5, adenosylcobyrinic acid tetraamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.088
ATP
pH 7.5, adenosylcobyrinic acid pentaamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.12
ATP
pH 7.5, adenosylcobyrinic acid tetraamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
20.4
ammonia
pH 7.5, adenosylcobyrinic acid a,c-diamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
-20°C, 0.1 M Tris hydrochloride, pH 7.5, 100% activity is lost after 48 h
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
-20°C, enzyme can be stored in the eluate from purification step v without detectable loss of activity
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0°C, 0.1 M Tris hydrochloride, pH 7.5, 100% activity is lost after 48 h
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
-20°C or 0°C, purified enzyme, 0.1 M Tris hydrochloride, pH 7.5, 48 h, 100% loss of activity
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
-20°C, purified enzyme, 50 mM Tris hydrochloride, pH 7.5, 0.1 M sodium chloride, 1 mM dithiothreitol, 20% glycerol, several weeks, stable
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
5'-deoxy-5'-adenosyl-cobyrinic acid + 2 ATP + 2 glutamine + 2 H2O
651618
Pseudomonas denitrificans (nom. rej.)
5'-deoxy-5'-adenosyl-cobyrinic acid ac-diamide + 2 ADP + 2 phosphate + 2 glutamate
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
5'-deoxy-5'-adenosyl-cobyrinic acid + 2 ATP + 2 glutamine + 2 H2O
cobalamin biosynthetic pathway
651618
Pseudomonas denitrificans (nom. rej.)
5'-deoxy-5'-adenosyl-cobyrinic acid ac-diamide + 2 ADP + 2 phosphate + 2 glutamate
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
5'-deoxy-5'-adenosyl-cobyrinic acid + ATP + ammonia + H2O
651618
Pseudomonas denitrificans (nom. rej.)
5'-deoxy-5'-adenosyl-cobyrinic acid ac-diamide + ADP + phosphate + ?
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
adenosyl-cobyrinic acid pentaamide + ATP + glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
ADP + glutamate + phosphate + ?
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
adenosyl-cobyrinic acid tetraamide + ATP + glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
ADP + glutamate + phosphate + ?
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
adenosyl-cobyrinic acid triamide + ATP + glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
ADP + glutamate + phosphate + ?
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
ATP + adenosyl-cobyrinic acid a,c-diamide + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
?
6.3.5.9
additional information
adenosyl-cobyric acid and hydrogenobyrinic acid a,c-diamide are no substrates
651618
Pseudomonas denitrificans (nom. rej.)
?
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.10
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
adenosylcobyric acid + ADP + phosphate + L-glutamate
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.10
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
adenosylcobyric acid + ADP + phosphate + L-glutamate
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + ammonia + H2O
ammonia can replace L-glutamine as amide group donor, but L-glutamine is preferred
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + ?
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
biosynthesis of vitamin B12, cobalamin biosynthetic pathway
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
enzyme catalyzes the stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide, four-step amidation sequence from cobyrinic acid a,c-diamide to cobyric acid via the formation of corbyrinic acid triamide, tetraamide, and pentaamide intermediates, the amidations are carried out in a specific order, enzyme is specific to coenzyme forms of substrates, no amidation of the carboxyl group at position f, L-glutamine is the preferred amide group donor
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
biosynthesis of vitamin B12, cobalamin biosynthetic pathway
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
enzyme catalyzes the stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide, four-step amidation sequence from cobyrinic acid a,c-diamide to cobyric acid via the formation of corbyrinic acid triamide, tetraamide, and pentaamide intermediates, the amidations are carried out in a specific order, enzyme is specific to coenzyme forms of substrates, no amidation of the carboxyl group at position f, L-glutamine is the preferred amide group donor
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid pentaamide + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid tetraamide + L-glutamine + H2O
two-step amidation
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid triamide + L-glutamine + H2O
three-step amidation
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
cobinic acid a,c-diamide + ATP + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
cobinamide + ADP + phosphate + L-glutamate
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.10
cobinic acid a,c-diamide + ATP + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
cobinamide + ADP + phosphate + L-glutamate
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
?
6.3.5.10
additional information
no amidation of adenosylcobyric acid, hydrogenobyrinic acid a,c-diamide, monocyano or diaqua form of cobyrinic acid a,c-diamide
651618
Pseudomonas denitrificans (nom. rej.)
?
?
6.3.5.10
additional information
no amidation of adenosylcobyric acid, hydrogenobyrinic acid a,c-diamide, monocyano or diaqua form of cobyrinic acid a,c-diamide
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
?
?
6.3.5.9
0.00104
adenosyl-cobyrinic acid a,c-diamide
pH 7.5, 20°C
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.00121
adenosyl-cobyrinic acid tetraamide
pH 7.5, 20°C
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.00122
adenosyl-cobyrinic acid triamide
pH 7.5, 20°C
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.00125
adenosyl-cobyrinic acid pentaamide
pH 7.5, 20°C
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.011
ATP
pH 7.5, 20°C, adenosyl-cobyrinic acid a,c-diamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.033
L-glutamine
pH 7.5, 20°C, adenosyl-cobyrinic acid triamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.035
ATP
pH 7.5, 20°C, adenosyl-cobyrinic acid triamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.045
L-glutamine
pH 7.5, 20°C, adenosyl-cobyrinic acid a,c-diamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.049
L-glutamine
pH 7.5, 20°C, adenosyl-cobyrinic acid pentaamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.064
L-glutamine
pH 7.5, 20°C, adenosyl-cobyrinic acid tetraamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.088
ATP
pH 7.5, 20°C, adenosyl-cobyrinic acid pentaamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0.12
ATP
pH 7.5, 20°C, adenosyl-cobyrinic acid tetraamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
20
ammonia
pH 7.5, 20°C
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
additional information
additional information
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.00104
adenosylcobyrinic acid a,c-diamide
pH 7.5
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.00121
adenosylcobyrinic acid tetraamide
pH 7.5
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.00122
adenosylcobyrinic acid triamide
pH 7.5
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.00125
adenosylcobyrinic acid pentaamide
pH 7.5
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.011
ATP
pH 7.5, adenosylcobyrinic acid a,c-diamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.033
L-glutamine
pH 7.5, adenosylcobyrinic acid triamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.035
ATP
pH 7.5, adenosylcobyrinic acid triamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.045
L-glutamine
pH 7.5, adenosylcobyrinic acid a,c-diamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.049
L-glutamine
pH 7.5, adenosylcobyrinic acid pentaamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.064
L-glutamine
pH 7.5, adenosylcobyrinic acid tetraamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.088
ATP
pH 7.5, adenosylcobyrinic acid pentaamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
0.12
ATP
pH 7.5, adenosylcobyrinic acid tetraamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
20.4
ammonia
pH 7.5, adenosylcobyrinic acid a,c-diamide as substrate
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
-20°C, 0.1 M Tris hydrochloride, pH 7.5, 100% activity is lost after 48 h
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
-20°C, enzyme can be stored in the eluate from purification step v without detectable loss of activity
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
0°C, 0.1 M Tris hydrochloride, pH 7.5, 100% activity is lost after 48 h
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
-20°C or 0°C, purified enzyme, 0.1 M Tris hydrochloride, pH 7.5, 48 h, 100% loss of activity
Pseudomonas denitrificans (nom. rej.)
6.3.5.10
-20°C, purified enzyme, 50 mM Tris hydrochloride, pH 7.5, 0.1 M sodium chloride, 1 mM dithiothreitol, 20% glycerol, several weeks, stable
Pseudomonas denitrificans (nom. rej.)
6.3.5.9
5'-deoxy-5'-adenosyl-cobyrinic acid + 2 ATP + 2 glutamine + 2 H2O
651618
Pseudomonas denitrificans (nom. rej.)
5'-deoxy-5'-adenosyl-cobyrinic acid ac-diamide + 2 ADP + 2 phosphate + 2 glutamate
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
5'-deoxy-5'-adenosyl-cobyrinic acid + 2 ATP + 2 glutamine + 2 H2O
cobalamin biosynthetic pathway
651618
Pseudomonas denitrificans (nom. rej.)
5'-deoxy-5'-adenosyl-cobyrinic acid ac-diamide + 2 ADP + 2 phosphate + 2 glutamate
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
5'-deoxy-5'-adenosyl-cobyrinic acid + ATP + ammonia + H2O
651618
Pseudomonas denitrificans (nom. rej.)
5'-deoxy-5'-adenosyl-cobyrinic acid ac-diamide + ADP + phosphate + ?
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
adenosyl-cobyrinic acid pentaamide + ATP + glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
ADP + glutamate + phosphate + ?
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
adenosyl-cobyrinic acid tetraamide + ATP + glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
ADP + glutamate + phosphate + ?
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
adenosyl-cobyrinic acid triamide + ATP + glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
ADP + glutamate + phosphate + ?
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.9
ATP + adenosyl-cobyrinic acid a,c-diamide + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
?
6.3.5.9
additional information
adenosyl-cobyric acid and hydrogenobyrinic acid a,c-diamide are no substrates
651618
Pseudomonas denitrificans (nom. rej.)
?
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.10
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
adenosylcobyric acid + ADP + phosphate + L-glutamate
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.10
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
adenosylcobyric acid + ADP + phosphate + L-glutamate
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + ammonia + H2O
ammonia can replace L-glutamine as amide group donor, but L-glutamine is preferred
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + ?
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
biosynthesis of vitamin B12, cobalamin biosynthetic pathway
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
enzyme catalyzes the stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide, four-step amidation sequence from cobyrinic acid a,c-diamide to cobyric acid via the formation of corbyrinic acid triamide, tetraamide, and pentaamide intermediates, the amidations are carried out in a specific order, enzyme is specific to coenzyme forms of substrates, no amidation of the carboxyl group at position f, L-glutamine is the preferred amide group donor
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
biosynthesis of vitamin B12, cobalamin biosynthetic pathway
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
enzyme catalyzes the stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide, four-step amidation sequence from cobyrinic acid a,c-diamide to cobyric acid via the formation of corbyrinic acid triamide, tetraamide, and pentaamide intermediates, the amidations are carried out in a specific order, enzyme is specific to coenzyme forms of substrates, no amidation of the carboxyl group at position f, L-glutamine is the preferred amide group donor
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid pentaamide + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid tetraamide + L-glutamine + H2O
two-step amidation
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
ATP + adenosylcobyrinic acid triamide + L-glutamine + H2O
three-step amidation
651618
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + adenosylcobyric acid + L-glutamate
?
6.3.5.10
cobinic acid a,c-diamide + ATP + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.)
cobinamide + ADP + phosphate + L-glutamate
651618
Pseudomonas denitrificans (nom. rej.)
?
6.3.5.10
cobinic acid a,c-diamide + ATP + L-glutamine + H2O
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
cobinamide + ADP + phosphate + L-glutamate
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
?
6.3.5.10
additional information
no amidation of adenosylcobyric acid, hydrogenobyrinic acid a,c-diamide, monocyano or diaqua form of cobyrinic acid a,c-diamide
651618
Pseudomonas denitrificans (nom. rej.)
?
?
6.3.5.10
additional information
no amidation of adenosylcobyric acid, hydrogenobyrinic acid a,c-diamide, monocyano or diaqua form of cobyrinic acid a,c-diamide
651618
Pseudomonas denitrificans (nom. rej.) SC510 RifT
?
?