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Literature summary extracted from

  • O'Reilly, C.; Turner, P.D.
    The nitrilase family of CN hydrolyzing enzymes - a comparative study (2003), J. Appl. Microbiol., 95, 1161-1174.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.66 cyanide enzyme induction Stemphylium loti

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 DNA and amino acid sequence determination, genes of the 3 isozymes are clustered on chromosome 3 Arabidopsis thaliana
4.2.1.66 DNA sequence analysis Fusarium lateritium
4.2.1.66 DNA sequence analysis Fusarium solani
4.2.1.66 DNA sequence analysis Microdochium sorghi

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.66 D275E site-directed mutagenesis, 0-29% reduced activity compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 F170L site-directed mutagenesis, inactive mutant compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 K136R site-directed mutagenesis, 52% reduced activity compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 M302S site-directed mutagenesis, 22% reduced activity compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 S13A site-directed mutagenesis, 19-47% reduced activity compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 T12P site-directed mutagenesis, inactive mutant Fusarium lateritium
4.2.1.66 T12Q site-directed mutagenesis, 10-48% reduced activity compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 V281A site-directed mutagenesis, 8% reduced activity compared to the wild-type enzyme Fusarium lateritium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 32000
-
 x * 32000, strain ATCC 8750, x * 44000, strain JM3 Alcaligenes faecalis
3.5.5.1 37000
-
 x * 37000 Fusarium oxysporum
3.5.5.1 38000
-
 x * 38000 Comamonas testosteroni
3.5.5.1 40000
-
 x * 40000 Rhodococcus sp. (in: high G+C Gram-positive bacteria)
3.5.5.1 40000
-
 x * 40000, strain J1 Rhodococcus rhodochrous
3.5.5.1 41000
-
 strain SI Pseudomonas sp.
3.5.5.1 41000
-
 x * 41000 Rhodococcus rhodochrous
3.5.5.1 41000
-
 x * 41000 + x * 72000, enzyme + groEL Aeribacillus pallidus
3.5.5.1 44000
-
 x * 32000, strain ATCC 8750, x * 44000, strain JM3 Alcaligenes faecalis
3.5.5.1 45000
-
 strain PA-34 Rhodococcus rhodochrous
3.5.5.1 45000
-
 x * 45000, strain NCIB11216 and strain NCIB11215 Nocardia sp.
3.5.5.1 72000
-
 x * 41000 + x * 72000, enzyme + groEL Aeribacillus pallidus
3.5.5.1 74000
-
-
 Klebsiella pneumoniae
3.5.5.1 76000
-
 x * 76000 Fusarium solani
3.5.5.1 260000
-
 strain JM3 Alcaligenes faecalis
3.5.5.1 410000
-
 strain J1 Rhodococcus rhodochrous
3.5.5.1 460000
-
 strain ATCC 8750 Alcaligenes faecalis
3.5.5.1 550000
-
-
 Fusarium oxysporum
3.5.5.1 560000
-
 strain NCIB11216 and strain NCIB11215 Nocardia sp.
3.5.5.1 560000
-
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria)
3.5.5.1 580000
-
-
 Acinetobacter sp.
3.5.5.1 600000
-
-
 Aeribacillus pallidus
3.5.5.1 620000
-
-
 Fusarium solani
3.5.5.1 650000
-
-
 Rhodococcus rhodochrous
4.2.1.66 40000
-
 x * 40000 Fusarium solani
4.2.1.66 40000
-
 x * 40000 Fusarium oxysporum
4.2.1.66 40000
-
 x * 40000 Microdochium sorghi
4.2.1.66 40000
-
 x * 40000 Fusarium lateritium
4.2.1.66 40000
-
 n * 40000 Plenodomus lingam
4.2.1.66 160000
-
-
 Plenodomus lingam
4.2.1.66 300000
-
 above Fusarium solani
4.2.1.66 300000
-
 above Fusarium lateritium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 bromoxynil + H2O Klebsiella pneumoniae enzyme utilizes the herbicide substrate as nitrogen source ?
-
 ?
3.5.5.1 indole-3-acetonitrile + H2O Arabidopsis thaliana isozymes are involved in indole-3-acetic acid production indole-3-acetic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Acidovorax facilis
-
-
-
3.5.5.1 Acidovorax facilis 72W
-
-
-
3.5.5.1 Acinetobacter sp.
-
-
-
3.5.5.1 Aeribacillus pallidus
-
-
-
3.5.5.1 Aeribacillus pallidus Dac521
-
-
-
3.5.5.1 Alcaligenes faecalis
-
 and ATCC 8750
-
3.5.5.1 Alcaligenes faecalis JM3
-
 and ATCC 8750
-
3.5.5.1 Arabidopsis thaliana
-
 3 isoenzymes NIT1-3
-
3.5.5.1 Bacillus sp. (in: firmicutes)
-
-
-
3.5.5.1 Bacillus sp. (in: firmicutes) OxB-1
-
-
-
3.5.5.1 Brassica rapa
-
 ssp. pehinensis, chinese cabbage
-
3.5.5.1 Comamonas testosteroni
-
-
-
3.5.5.1 Fusarium oxysporum
-
 f.sp. melonis
-
3.5.5.1 Fusarium solani
-
-
-
3.5.5.1 Fusarium solani IMI196840
-
-
-
3.5.5.1 Hordeum vulgare
-
-
-
3.5.5.1 Klebsiella pneumoniae
-
 ssp. ozaenae
-
3.5.5.1 Nocardia sp.
-
 i.e. Rhodococcus sp. strain NCIB11216 and NCIB11215
-
3.5.5.1 Pseudomonas sp.
-
 strain DSM 7155 and strain SI
-
3.5.5.1 Rhodococcus rhodochrous
-
-
-
3.5.5.1 Rhodococcus rhodochrous
-
 strains PA-34 and J1
-
3.5.5.1 Rhodococcus rhodochrous K22
-
-
-
3.5.5.1 Rhodococcus sp. (in: high G+C Gram-positive bacteria)
-
 strain ATCC39484
-
3.5.5.1 Rhodococcus sp. (in: high G+C Gram-positive bacteria) ATCC 39484
-
 strain ATCC39484
-
4.2.1.66 Fusarium lateritium
-
-
-
4.2.1.66 Fusarium oxysporum
-
-
-
4.2.1.66 Fusarium oxysporum N-10
-
-
-
4.2.1.66 Fusarium solani
-
-
-
4.2.1.66 Fusarium solani IMI 196840
-
-
-
4.2.1.66 Microdochium sorghi
-
-
-
4.2.1.66 Plenodomus lingam
-
-
-
4.2.1.66 Stemphylium loti
-
 pathogen of the cyanogenic plant Lotus corniculatus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1
-
 Klebsiella pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.66 formamide = cyanide + H2O reaction mechanism Stemphylium loti
a
4.2.1.66 formamide = cyanide + H2O reaction mechanism Fusarium solani
a
4.2.1.66 formamide = cyanide + H2O reaction mechanism Fusarium oxysporum
a
4.2.1.66 formamide = cyanide + H2O reaction mechanism Fusarium lateritium
a
4.2.1.66 formamide = cyanide + H2O reaction mechanism Microdochium sorghi
a
4.2.1.66 formamide = cyanide + H2O reaction mechanism Plenodomus lingam
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 1,4-benzodinitrile + H2O
-
 Fusarium solani ?
-
 ?
3.5.5.1 1,4-benzodinitrile + H2O
-
 Fusarium solani IMI 196840 ?
-
 ?
3.5.5.1 1,4-benzodinitrile + H2O
-
 Fusarium solani IMI196840 ?
-
 ?
3.5.5.1 2-bromobenzonitrile + H2O best substrate of strain NCIB11216 and strain NCIB11215 Nocardia sp. 2-bromobenzoic acid + NH3
-
 ?
3.5.5.1 2-furanocarbonitrile + H2O
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria) 2-furanocarboxylic acid + NH3
-
 ?
3.5.5.1 2-furanocarbonitrile + H2O
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria) ATCC 39484 2-furanocarboxylic acid + NH3
-
 ?
3.5.5.1 2-nitrobenzonitrile + H2O strain NCIB11215 Nocardia sp. 2-nitrobenzoic acid + NH3
-
 ?
3.5.5.1 2-thiophenacetonitrile + H2O strain JM3 Alcaligenes faecalis 2-thiophenacetic acid + NH3
-
 ?
3.5.5.1 2-thiophenacetonitrile + H2O strain JM3 Alcaligenes faecalis JM3 2-thiophenacetic acid + NH3
-
 ?
3.5.5.1 acetonitrile + H2O strain SI Pseudomonas sp. acetic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O low activity Aeribacillus pallidus acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O
-
 Comamonas testosteroni acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O best substrate of strain SI Pseudomonas sp. acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O best substrate Rhodococcus rhodochrous acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O
-
 Fusarium oxysporum acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O
-
 Fusarium solani acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O
-
 Rhodococcus rhodochrous acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O high activity Acinetobacter sp. acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O best substrate Rhodococcus rhodochrous K22 acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O
-
 Rhodococcus rhodochrous K22 acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O
-
 Fusarium solani IMI 196840 acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O
-
 Fusarium solani IMI196840 acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O low activity Aeribacillus pallidus Dac521 acrylic acid + NH3
-
 ?
3.5.5.1 adiponitrile + H2O
-
 Comamonas testosteroni adipic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Aeribacillus pallidus benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O low activity Comamonas testosteroni benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O very low activity Klebsiella pneumoniae benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O strain SI, low activity Pseudomonas sp. benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O high activity in strain NCIB11216 and strain NCIB11215 Nocardia sp. benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria) benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O low activity Rhodococcus rhodochrous benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Fusarium oxysporum benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O low activity Fusarium solani benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Rhodococcus rhodochrous benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Acinetobacter sp. benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O strain ATCC 8750 Alcaligenes faecalis benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O low activity Rhodococcus rhodochrous K22 benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Rhodococcus rhodochrous K22 benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O strain ATCC 8750 Alcaligenes faecalis JM3 benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O low activity Fusarium solani IMI 196840 benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O low activity Fusarium solani IMI196840 benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Aeribacillus pallidus Dac521 benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria) ATCC 39484 benzoic acid + NH3
-
 ?
3.5.5.1 bromoxynil + H2O enzyme utilizes the herbicide substrate as nitrogen source Klebsiella pneumoniae ?
-
 ?
3.5.5.1 bromoxynil + H2O 3',5-dihalogenated 4-hydroxybenzonitrile compound, high activity, substrate is a herbicide Klebsiella pneumoniae ?
-
 ?
3.5.5.1 bromoxynil + H2O strain NCIB11215, very low activity, 3',5-dihalogenated 4-hydroxybenzonitrile compound Nocardia sp. ?
-
 ?
3.5.5.1 chloroxynil + H2O 3',5-dihalogenated 4-hydroxybenzonitrile compound Klebsiella pneumoniae ?
-
 ?
3.5.5.1 crotononitrile + 2 H2O
-
 Aeribacillus pallidus crotonic acid + NH3
-
 ?
3.5.5.1 crotononitrile + 2 H2O
-
 Aeribacillus pallidus Dac521 crotonic acid + NH3
-
 ?
3.5.5.1 crotononitrile + H2O
-
 Fusarium oxysporum ?
-
 ?
3.5.5.1 crotononitrile + H2O
-
 Rhodococcus rhodochrous ?
-
 ?
3.5.5.1 crotononitrile + H2O
-
 Rhodococcus rhodochrous K22 ?
-
 ?
3.5.5.1 indole-3-acetonitrile + H2O isozymes are involved in indole-3-acetic acid production Arabidopsis thaliana indole-3-acetic acid + NH3
-
 ?
3.5.5.1 indole-3-acetonitrile + H2O
-
 Arabidopsis thaliana indole-3-acetic acid + NH3
-
 ?
3.5.5.1 ioxynil + H2O 3',5-dihalogenated 4-hydroxybenzonitrile compound Klebsiella pneumoniae ?
-
 ?
3.5.5.1 ioxynil + H2O 3',5-dihalogenated 4-hydroxybenzonitrile compound, strain NCIB11215 Nocardia sp. ?
-
 ?
3.5.5.1 mandelonitrile + 2 H2O best substrate of strain ATCC 8750, low activity in strain JM3 Alcaligenes faecalis mandelic acid + NH3
-
 ?
3.5.5.1 mandelonitrile + 2 H2O best substrate of strain ATCC 8750, low activity in strain JM3 Alcaligenes faecalis JM3 mandelic acid + NH3
-
 ?
3.5.5.1 additional information substrate specificity, overview Aeribacillus pallidus ?
-
 ?
3.5.5.1 additional information substrate specificity, overview Comamonas testosteroni ?
-
 ?
3.5.5.1 additional information stricter substrate specificity compared to nitrilases from other species, strong preference for 4-hydroxybenzonitriles Klebsiella pneumoniae ?
-
 ?
3.5.5.1 additional information substrate specificity, enzyme catalyzes a range of 2-pyridones with rates of 1-118% relative to ricinine, overview Pseudomonas sp. ?
-
 ?
3.5.5.1 additional information substrate specificity, overview Nocardia sp. ?
-
 ?
3.5.5.1 additional information substrate specificity, overview Fusarium oxysporum ?
-
 ?
3.5.5.1 additional information substrate specificity, overview Fusarium solani ?
-
 ?
3.5.5.1 additional information substrate specificity Hordeum vulgare ?
-
 ?
3.5.5.1 additional information substrate specificity Brassica rapa ?
-
 ?
3.5.5.1 additional information substrate specificity, overview Rhodococcus rhodochrous ?
-
 ?
3.5.5.1 additional information substrate specificity, overview Acidovorax facilis ?
-
 ?
3.5.5.1 additional information substrate specificity, overview Acinetobacter sp. ?
-
 ?
3.5.5.1 additional information substrate specificity, overview Alcaligenes faecalis ?
-
 ?
3.5.5.1 additional information substrate specificity, overview Acidovorax facilis 72W ?
-
 ?
3.5.5.1 additional information substrate specificity, overview Alcaligenes faecalis JM3 ?
-
 ?
3.5.5.1 additional information substrate specificity, overview Aeribacillus pallidus Dac521 ?
-
 ?
3.5.5.1 N-methyl-3-cyano-4-methoxy-2-pyridone + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.5.1 p-aminobenzyl cyanide + H2O strains ATCC 8750 and JM3 Alcaligenes faecalis ?
-
 ?
3.5.5.1 p-aminobenzyl cyanide + H2O strains ATCC 8750 and JM3 Alcaligenes faecalis JM3 ?
-
 ?
3.5.5.1 ricinine + H2O
-
 Pseudomonas sp. ?
-
 ?
4.2.1.66 acrylonitrile + H2O about 0.05% of the activity with HCN Fusarium oxysporum ?
-
 ?
4.2.1.66 acrylonitrile + H2O about 0.05% of the activity with HCN Fusarium oxysporum N-10 ?
-
 ?
4.2.1.66 crotononitrile + H2O about 0.05% of the activity with HCN Fusarium oxysporum ?
-
 ?
4.2.1.66 crotononitrile + H2O about 0.05% of the activity with HCN Fusarium oxysporum N-10 ?
-
 ?
4.2.1.66 HCN + H2O
-
 Fusarium solani formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Fusarium oxysporum formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Stemphylium loti formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Fusarium lateritium formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Plenodomus lingam formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Microdochium sorghi formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Fusarium oxysporum N-10 formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Fusarium solani IMI 196840 formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Fusarium solani IMI196840 formamide
-
 ?
4.2.1.66 methacrylonitrile + H2O about 0.05% of the activity with HCN Fusarium oxysporum ?
-
 ?
4.2.1.66 methacrylonitrile + H2O about 0.05% of the activity with HCN Fusarium oxysporum N-10 ?
-
 ?
4.2.1.66 additional information hydrolysis of a metal-cyano complex, tetracyanonickelate Fusarium solani ?
-
 ?
4.2.1.66 additional information low hydrolytic activity with a metal-cyano complex, tetracyanonickelate, the enzyme also shows nitrilase activity Fusarium oxysporum ?
-
 ?
4.2.1.66 additional information low hydrolytic activity with a metal-cyano complex, tetracyanonickelate, the enzyme also shows nitrilase activity Fusarium oxysporum N-10 ?
-
 ?
4.2.1.66 additional information hydrolysis of a metal-cyano complex, tetracyanonickelate Fusarium solani IMI 196840 ?
-
 ?
4.2.1.66 additional information hydrolysis of a metal-cyano complex, tetracyanonickelate Fusarium solani IMI196840 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 ? x * 41000 + x * 72000, enzyme + groEL Aeribacillus pallidus
3.5.5.1 ? x * 45000, strain NCIB11216 and strain NCIB11215 Nocardia sp.
3.5.5.1 ? x * 40000 Rhodococcus sp. (in: high G+C Gram-positive bacteria)
3.5.5.1 ? x * 41000 Rhodococcus rhodochrous
3.5.5.1 ? x * 37000 Fusarium oxysporum
3.5.5.1 ? x * 76000 Fusarium solani
3.5.5.1 ? x * 40000, strain J1 Rhodococcus rhodochrous
3.5.5.1 ? x * 41000-43000 Acinetobacter sp.
3.5.5.1 ? x * 32000, strain ATCC 8750, x * 44000, strain JM3 Alcaligenes faecalis
3.5.5.1 dimer 2 x 37000 Klebsiella pneumoniae
3.5.5.1 monomer 1 x 41000, strain SI Pseudomonas sp.
3.5.5.1 monomer 1 x * 45000, strain PA-34 Rhodococcus rhodochrous
3.5.5.1 oligomer x * 38000 Comamonas testosteroni
4.2.1.66 oligomer x * 40000 Fusarium solani
4.2.1.66 oligomer x * 40000 Fusarium oxysporum
4.2.1.66 oligomer x * 40000 Fusarium lateritium
4.2.1.66 oligomer x * 40000 Microdochium sorghi
4.2.1.66 tetramer n * 40000 Plenodomus lingam

Synonyms

EC Number Synonyms Comment Organism
4.2.1.66 formamide hydrolase
-
 Stemphylium loti
4.2.1.66 formamide hydrolase
-
 Fusarium solani
4.2.1.66 formamide hydrolase
-
 Fusarium oxysporum
4.2.1.66 formamide hydrolase
-
 Fusarium lateritium
4.2.1.66 formamide hydrolase
-
 Microdochium sorghi
4.2.1.66 formamide hydrolase
-
 Plenodomus lingam
4.2.1.66 additional information the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes Stemphylium loti
4.2.1.66 additional information the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes Fusarium solani
4.2.1.66 additional information the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes Fusarium oxysporum
4.2.1.66 additional information the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes Fusarium lateritium
4.2.1.66 additional information the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes Microdochium sorghi
4.2.1.66 additional information the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes Plenodomus lingam

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 30
-
 strain NCIB11216 and strain NCIB11215 Nocardia sp.
3.5.5.1 30
-
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria)
3.5.5.1 35
-
-
 Klebsiella pneumoniae
3.5.5.1 35
-
 strain PA-34 Rhodococcus rhodochrous
3.5.5.1 40 45 strain ATCC 8750 Alcaligenes faecalis
3.5.5.1 40
-
-
 Fusarium oxysporum
3.5.5.1 44
-
 strain JM3 Alcaligenes faecalis
3.5.5.1 45
-
 strain J1 Rhodococcus rhodochrous
3.5.5.1 50
-
-
 Acinetobacter sp.
3.5.5.1 55
-
-
 Rhodococcus rhodochrous
3.5.5.1 65
-
-
 Aeribacillus pallidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 5.5
-
-
 Rhodococcus rhodochrous
3.5.5.1 6 9
-
 Aeribacillus pallidus
3.5.5.1 6 11
-
 Fusarium oxysporum
3.5.5.1 7 9.5 strain NCIB11215 Nocardia sp.
3.5.5.1 7.5
-
-
 Rhodococcus sp. (in: high G+C Gram-positive bacteria)
3.5.5.1 7.5
-
 strain PA-34 Rhodococcus rhodochrous
3.5.5.1 7.5
-
 strains ATCC 8750 and JM3 Alcaligenes faecalis
3.5.5.1 7.6
-
 strain J1 Rhodococcus rhodochrous
3.5.5.1 7.8 9.1
-
 Fusarium solani
3.5.5.1 8
-
 strain NCIB11216 Nocardia sp.
3.5.5.1 8
-
-
 Acinetobacter sp.
3.5.5.1 9.2
-
-
 Klebsiella pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.66 additional information
-
 broad range Stemphylium loti