Any feedback?
Literature summary extracted from
- The nitrilase family of CN hydrolyzing enzymes - a comparative study (2003), J. Appl. Microbiol., 95, 1161-1174.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.66 | cyanide | enzyme induction | Stemphylium loti |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.5.1 | DNA and amino acid sequence determination, genes of the 3 isozymes are clustered on chromosome 3 | Arabidopsis thaliana |
| 4.2.1.66 | DNA sequence analysis | Fusarium solani |
| 4.2.1.66 | DNA sequence analysis | Microdochium sorghi |
| 4.2.1.66 | DNA sequence analysis | Fusarium lateritium |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.66 | D275E | site-directed mutagenesis, 0-29% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
| 4.2.1.66 | F170L | site-directed mutagenesis, inactive mutant compared to the wild-type enzyme | Fusarium lateritium |
| 4.2.1.66 | K136R | site-directed mutagenesis, 52% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
| 4.2.1.66 | M302S | site-directed mutagenesis, 22% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
| 4.2.1.66 | S13A | site-directed mutagenesis, 19-47% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
| 4.2.1.66 | T12P | site-directed mutagenesis, inactive mutant | Fusarium lateritium |
| 4.2.1.66 | T12Q | site-directed mutagenesis, 10-48% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
| 4.2.1.66 | V281A | site-directed mutagenesis, 8% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 32000 | - |
x * 32000, strain ATCC 8750, x * 44000, strain JM3 | Alcaligenes faecalis |
| 3.5.5.1 | 37000 | - |
x * 37000 | Fusarium oxysporum |
| 3.5.5.1 | 38000 | - |
x * 38000 | Comamonas testosteroni |
| 3.5.5.1 | 40000 | - |
x * 40000 | Rhodococcus sp. |
| 3.5.5.1 | 40000 | - |
x * 40000, strain J1 | Rhodococcus rhodochrous |
| 3.5.5.1 | 41000 | - |
strain SI | Pseudomonas sp. |
| 3.5.5.1 | 41000 | - |
x * 41000 | Rhodococcus rhodochrous |
| 3.5.5.1 | 41000 | - |
x * 41000 + x * 72000, enzyme + groEL | Aeribacillus pallidus |
| 3.5.5.1 | 44000 | - |
x * 32000, strain ATCC 8750, x * 44000, strain JM3 | Alcaligenes faecalis |
| 3.5.5.1 | 45000 | - |
strain PA-34 | Rhodococcus rhodochrous |
| 3.5.5.1 | 45000 | - |
x * 45000, strain NCIB11216 and strain NCIB11215 | Nocardia sp. |
| 3.5.5.1 | 72000 | - |
x * 41000 + x * 72000, enzyme + groEL | Aeribacillus pallidus |
| 3.5.5.1 | 74000 | - |
- |
Klebsiella pneumoniae |
| 3.5.5.1 | 76000 | - |
x * 76000 | Fusarium solani |
| 3.5.5.1 | 260000 | - |
strain JM3 | Alcaligenes faecalis |
| 3.5.5.1 | 410000 | - |
strain J1 | Rhodococcus rhodochrous |
| 3.5.5.1 | 460000 | - |
strain ATCC 8750 | Alcaligenes faecalis |
| 3.5.5.1 | 550000 | - |
- |
Fusarium oxysporum |
| 3.5.5.1 | 560000 | - |
- |
Rhodococcus sp. |
| 3.5.5.1 | 560000 | - |
strain NCIB11216 and strain NCIB11215 | Nocardia sp. |
| 3.5.5.1 | 580000 | - |
- |
Acinetobacter sp. |
| 3.5.5.1 | 600000 | - |
- |
Aeribacillus pallidus |
| 3.5.5.1 | 620000 | - |
- |
Fusarium solani |
| 3.5.5.1 | 650000 | - |
- |
Rhodococcus rhodochrous |
| 4.2.1.66 | 40000 | - |
x * 40000 | Fusarium solani |
| 4.2.1.66 | 40000 | - |
x * 40000 | Fusarium oxysporum |
| 4.2.1.66 | 40000 | - |
x * 40000 | Microdochium sorghi |
| 4.2.1.66 | 40000 | - |
x * 40000 | Fusarium lateritium |
| 4.2.1.66 | 40000 | - |
n * 40000 | Leptosphaeria maculans |
| 4.2.1.66 | 160000 | - |
- |
Leptosphaeria maculans |
| 4.2.1.66 | 300000 | - |
above | Fusarium solani |
| 4.2.1.66 | 300000 | - |
above | Fusarium lateritium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.1 | bromoxynil + H2O | Klebsiella pneumoniae | enzyme utilizes the herbicide substrate as nitrogen source | ? | - |
? | |
| 3.5.5.1 | indole-3-acetonitrile + H2O | Arabidopsis thaliana | isozymes are involved in indole-3-acetic acid production | indole-3-acetic acid + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.5.1 | Acidovorax facilis | - |
- |
- |
| 3.5.5.1 | Acidovorax facilis 72W | - |
- |
- |
| 3.5.5.1 | Acinetobacter sp. | - |
- |
- |
| 3.5.5.1 | Acinetobacter sp. AK226 | - |
- |
- |
| 3.5.5.1 | Aeribacillus pallidus | - |
- |
- |
| 3.5.5.1 | Aeribacillus pallidus Dac521 | - |
- |
- |
| 3.5.5.1 | Alcaligenes faecalis | - |
and ATCC 8750 | - |
| 3.5.5.1 | Alcaligenes faecalis JM3 | - |
and ATCC 8750 | - |
| 3.5.5.1 | Arabidopsis thaliana | - |
3 isoenzymes NIT1-3 | - |
| 3.5.5.1 | Bacillus sp. (in: Bacteria) | - |
- |
- |
| 3.5.5.1 | Bacillus sp. (in: Bacteria) OxB-1 | - |
- |
- |
| 3.5.5.1 | Brassica rapa | - |
ssp. pehinensis, chinese cabbage | - |
| 3.5.5.1 | Comamonas testosteroni | - |
- |
- |
| 3.5.5.1 | Fusarium oxysporum | - |
f.sp. melonis | - |
| 3.5.5.1 | Fusarium solani | - |
- |
- |
| 3.5.5.1 | Fusarium solani IMI196840 | - |
- |
- |
| 3.5.5.1 | Hordeum vulgare | - |
- |
- |
| 3.5.5.1 | Klebsiella pneumoniae | - |
ssp. ozaenae | - |
| 3.5.5.1 | Nocardia sp. | - |
i.e. Rhodococcus sp. strain NCIB11216 and NCIB11215 | - |
| 3.5.5.1 | Pseudomonas sp. | - |
strain DSM 7155 and strain SI | - |
| 3.5.5.1 | Rhodococcus rhodochrous | - |
- |
- |
| 3.5.5.1 | Rhodococcus rhodochrous | - |
strains PA-34 and J1 | - |
| 3.5.5.1 | Rhodococcus rhodochrous K22 | - |
- |
- |
| 3.5.5.1 | Rhodococcus sp. | - |
strain ATCC39484 | - |
| 3.5.5.1 | Rhodococcus sp. ATCC 39484 | - |
strain ATCC39484 | - |
| 4.2.1.66 | Fusarium lateritium | - |
- |
- |
| 4.2.1.66 | Fusarium oxysporum | - |
- |
- |
| 4.2.1.66 | Fusarium oxysporum N-10 | - |
- |
- |
| 4.2.1.66 | Fusarium solani | - |
- |
- |
| 4.2.1.66 | Fusarium solani IMI 196840 | - |
- |
- |
| 4.2.1.66 | Leptosphaeria maculans | - |
- |
- |
| 4.2.1.66 | Microdochium sorghi | - |
- |
- |
| 4.2.1.66 | Stemphylium loti | - |
pathogen of the cyanogenic plant Lotus corniculatus | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.5.1 | - |
Klebsiella pneumoniae |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.2.1.66 | formamide = cyanide + H2O | reaction mechanism | Fusarium solani | |
| 4.2.1.66 | formamide = cyanide + H2O | reaction mechanism | Fusarium oxysporum | |
| 4.2.1.66 | formamide = cyanide + H2O | reaction mechanism | Stemphylium loti | |
| 4.2.1.66 | formamide = cyanide + H2O | reaction mechanism | Microdochium sorghi | |
| 4.2.1.66 | formamide = cyanide + H2O | reaction mechanism | Fusarium lateritium | |
| 4.2.1.66 | formamide = cyanide + H2O | reaction mechanism | Leptosphaeria maculans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.1 | 1,4-benzodinitrile + H2O | - |
Fusarium solani | ? | - |
? | |
| 3.5.5.1 | 2-bromobenzonitrile + H2O | best substrate of strain NCIB11216 and strain NCIB11215 | Nocardia sp. | 2-bromobenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 2-furanocarbonitrile + H2O | - |
Rhodococcus sp. | 2-furanocarboxylic acid + NH3 | - |
? | |
| 3.5.5.1 | 2-furanocarbonitrile + H2O | - |
Rhodococcus sp. ATCC 39484 | 2-furanocarboxylic acid + NH3 | - |
? | |
| 3.5.5.1 | 2-nitrobenzonitrile + H2O | strain NCIB11215 | Nocardia sp. | 2-nitrobenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 2-thiophenacetonitrile + H2O | strain JM3 | Alcaligenes faecalis | 2-thiophenacetic acid + NH3 | - |
? | |
| 3.5.5.1 | 2-thiophenacetonitrile + H2O | strain JM3 | Alcaligenes faecalis JM3 | 2-thiophenacetic acid + NH3 | - |
? | |
| 3.5.5.1 | acetonitrile + H2O | strain SI | Pseudomonas sp. | acetic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | - |
Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | - |
Comamonas testosteroni | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | - |
Fusarium solani | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | - |
Fusarium oxysporum | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | best substrate | Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | low activity | Aeribacillus pallidus | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | high activity | Acinetobacter sp. | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | best substrate of strain SI | Pseudomonas sp. | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | - |
Fusarium solani IMI196840 | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | high activity | Acinetobacter sp. AK226 | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | - |
Rhodococcus rhodochrous K22 | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | best substrate | Rhodococcus rhodochrous K22 | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | - |
Fusarium solani IMI 196840 | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | low activity | Aeribacillus pallidus Dac521 | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | adiponitrile + H2O | - |
Comamonas testosteroni | adipic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | - |
Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | - |
Rhodococcus sp. | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | - |
Acinetobacter sp. | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | - |
Fusarium oxysporum | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | - |
Aeribacillus pallidus | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | very low activity | Klebsiella pneumoniae | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | low activity | Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | low activity | Comamonas testosteroni | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | low activity | Fusarium solani | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | high activity in strain NCIB11216 and strain NCIB11215 | Nocardia sp. | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | strain ATCC 8750 | Alcaligenes faecalis | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | strain SI, low activity | Pseudomonas sp. | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | low activity | Fusarium solani IMI196840 | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | - |
Rhodococcus sp. ATCC 39484 | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | - |
Acinetobacter sp. AK226 | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | - |
Rhodococcus rhodochrous K22 | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | low activity | Rhodococcus rhodochrous K22 | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | low activity | Fusarium solani IMI 196840 | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | strain ATCC 8750 | Alcaligenes faecalis JM3 | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | - |
Aeribacillus pallidus Dac521 | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | bromoxynil + H2O | enzyme utilizes the herbicide substrate as nitrogen source | Klebsiella pneumoniae | ? | - |
? | |
| 3.5.5.1 | bromoxynil + H2O | 3',5-dihalogenated 4-hydroxybenzonitrile compound, high activity, substrate is a herbicide | Klebsiella pneumoniae | ? | - |
? | |
| 3.5.5.1 | bromoxynil + H2O | strain NCIB11215, very low activity, 3',5-dihalogenated 4-hydroxybenzonitrile compound | Nocardia sp. | ? | - |
? | |
| 3.5.5.1 | chloroxynil + H2O | 3',5-dihalogenated 4-hydroxybenzonitrile compound | Klebsiella pneumoniae | ? | - |
? | |
| 3.5.5.1 | crotononitrile + 2 H2O | - |
Aeribacillus pallidus | crotonic acid + NH3 | - |
? | |
| 3.5.5.1 | crotononitrile + 2 H2O | - |
Aeribacillus pallidus Dac521 | crotonic acid + NH3 | - |
? | |
| 3.5.5.1 | crotononitrile + H2O | - |
Rhodococcus rhodochrous | ? | - |
? | |
| 3.5.5.1 | crotononitrile + H2O | - |
Fusarium oxysporum | ? | - |
? | |
| 3.5.5.1 | indole-3-acetonitrile + H2O | - |
Arabidopsis thaliana | indole-3-acetic acid + NH3 | - |
? | |
| 3.5.5.1 | indole-3-acetonitrile + H2O | isozymes are involved in indole-3-acetic acid production | Arabidopsis thaliana | indole-3-acetic acid + NH3 | - |
? | |
| 3.5.5.1 | ioxynil + H2O | 3',5-dihalogenated 4-hydroxybenzonitrile compound | Klebsiella pneumoniae | ? | - |
? | |
| 3.5.5.1 | ioxynil + H2O | 3',5-dihalogenated 4-hydroxybenzonitrile compound, strain NCIB11215 | Nocardia sp. | ? | - |
? | |
| 3.5.5.1 | mandelonitrile + 2 H2O | best substrate of strain ATCC 8750, low activity in strain JM3 | Alcaligenes faecalis | mandelic acid + NH3 | - |
? | |
| 3.5.5.1 | mandelonitrile + 2 H2O | best substrate of strain ATCC 8750, low activity in strain JM3 | Alcaligenes faecalis JM3 | mandelic acid + NH3 | - |
? | |
| 3.5.5.1 | additional information | substrate specificity | Hordeum vulgare | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity | Brassica rapa | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Alcaligenes faecalis | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Rhodococcus rhodochrous | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Comamonas testosteroni | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Nocardia sp. | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Acinetobacter sp. | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Acidovorax facilis | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Fusarium solani | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Fusarium oxysporum | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Aeribacillus pallidus | ? | - |
? | |
| 3.5.5.1 | additional information | stricter substrate specificity compared to nitrilases from other species, strong preference for 4-hydroxybenzonitriles | Klebsiella pneumoniae | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, enzyme catalyzes a range of 2-pyridones with rates of 1-118% relative to ricinine, overview | Pseudomonas sp. | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Fusarium solani IMI196840 | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Acinetobacter sp. AK226 | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Rhodococcus rhodochrous K22 | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Fusarium solani IMI 196840 | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Alcaligenes faecalis JM3 | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Aeribacillus pallidus Dac521 | ? | - |
? | |
| 3.5.5.1 | additional information | substrate specificity, overview | Acidovorax facilis 72W | ? | - |
? | |
| 3.5.5.1 | N-methyl-3-cyano-4-methoxy-2-pyridone + H2O | - |
Pseudomonas sp. | ? | - |
? | |
| 3.5.5.1 | p-aminobenzyl cyanide + H2O | strains ATCC 8750 and JM3 | Alcaligenes faecalis | ? | - |
? | |
| 3.5.5.1 | p-aminobenzyl cyanide + H2O | strains ATCC 8750 and JM3 | Alcaligenes faecalis JM3 | ? | - |
? | |
| 3.5.5.1 | ricinine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
| 4.2.1.66 | acrylonitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum | ? | - |
? | |
| 4.2.1.66 | acrylonitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum N-10 | ? | - |
? | |
| 4.2.1.66 | crotononitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum | ? | - |
? | |
| 4.2.1.66 | crotononitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum N-10 | ? | - |
? | |
| 4.2.1.66 | HCN + H2O | - |
Fusarium solani | formamide | - |
? | |
| 4.2.1.66 | HCN + H2O | - |
Fusarium oxysporum | formamide | - |
? | |
| 4.2.1.66 | HCN + H2O | - |
Stemphylium loti | formamide | - |
? | |
| 4.2.1.66 | HCN + H2O | - |
Microdochium sorghi | formamide | - |
? | |
| 4.2.1.66 | HCN + H2O | - |
Fusarium lateritium | formamide | - |
? | |
| 4.2.1.66 | HCN + H2O | - |
Leptosphaeria maculans | formamide | - |
? | |
| 4.2.1.66 | HCN + H2O | - |
Fusarium solani IMI196840 | formamide | - |
? | |
| 4.2.1.66 | HCN + H2O | - |
Fusarium solani IMI 196840 | formamide | - |
? | |
| 4.2.1.66 | HCN + H2O | - |
Fusarium oxysporum N-10 | formamide | - |
? | |
| 4.2.1.66 | methacrylonitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum | ? | - |
? | |
| 4.2.1.66 | methacrylonitrile + H2O | about 0.05% of the activity with HCN | Fusarium oxysporum N-10 | ? | - |
? | |
| 4.2.1.66 | additional information | hydrolysis of a metal-cyano complex, tetracyanonickelate | Fusarium solani | ? | - |
? | |
| 4.2.1.66 | additional information | low hydrolytic activity with a metal-cyano complex, tetracyanonickelate, the enzyme also shows nitrilase activity | Fusarium oxysporum | ? | - |
? | |
| 4.2.1.66 | additional information | hydrolysis of a metal-cyano complex, tetracyanonickelate | Fusarium solani IMI196840 | ? | - |
? | |
| 4.2.1.66 | additional information | hydrolysis of a metal-cyano complex, tetracyanonickelate | Fusarium solani IMI 196840 | ? | - |
? | |
| 4.2.1.66 | additional information | low hydrolytic activity with a metal-cyano complex, tetracyanonickelate, the enzyme also shows nitrilase activity | Fusarium oxysporum N-10 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.5.1 | ? | x * 40000 | Rhodococcus sp. |
| 3.5.5.1 | ? | x * 32000, strain ATCC 8750, x * 44000, strain JM3 | Alcaligenes faecalis |
| 3.5.5.1 | ? | x * 37000 | Fusarium oxysporum |
| 3.5.5.1 | ? | x * 40000, strain J1 | Rhodococcus rhodochrous |
| 3.5.5.1 | ? | x * 41000 | Rhodococcus rhodochrous |
| 3.5.5.1 | ? | x * 41000 + x * 72000, enzyme + groEL | Aeribacillus pallidus |
| 3.5.5.1 | ? | x * 41000-43000 | Acinetobacter sp. |
| 3.5.5.1 | ? | x * 45000, strain NCIB11216 and strain NCIB11215 | Nocardia sp. |
| 3.5.5.1 | ? | x * 76000 | Fusarium solani |
| 3.5.5.1 | dimer | 2 x 37000 | Klebsiella pneumoniae |
| 3.5.5.1 | monomer | 1 x * 45000, strain PA-34 | Rhodococcus rhodochrous |
| 3.5.5.1 | monomer | 1 x 41000, strain SI | Pseudomonas sp. |
| 3.5.5.1 | oligomer | x * 38000 | Comamonas testosteroni |
| 4.2.1.66 | oligomer | x * 40000 | Fusarium solani |
| 4.2.1.66 | oligomer | x * 40000 | Fusarium oxysporum |
| 4.2.1.66 | oligomer | x * 40000 | Microdochium sorghi |
| 4.2.1.66 | oligomer | x * 40000 | Fusarium lateritium |
| 4.2.1.66 | tetramer | n * 40000 | Leptosphaeria maculans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.66 | formamide hydrolase | - |
Fusarium solani |
| 4.2.1.66 | formamide hydrolase | - |
Fusarium oxysporum |
| 4.2.1.66 | formamide hydrolase | - |
Stemphylium loti |
| 4.2.1.66 | formamide hydrolase | - |
Microdochium sorghi |
| 4.2.1.66 | formamide hydrolase | - |
Fusarium lateritium |
| 4.2.1.66 | formamide hydrolase | - |
Leptosphaeria maculans |
| 4.2.1.66 | More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Fusarium solani |
| 4.2.1.66 | More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Fusarium oxysporum |
| 4.2.1.66 | More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Stemphylium loti |
| 4.2.1.66 | More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Microdochium sorghi |
| 4.2.1.66 | More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Fusarium lateritium |
| 4.2.1.66 | More | the enzyme belongs to the nitrilase family of CN hydrolyzing enzymes | Leptosphaeria maculans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 30 | - |
- |
Rhodococcus sp. |
| 3.5.5.1 | 30 | - |
strain NCIB11216 and strain NCIB11215 | Nocardia sp. |
| 3.5.5.1 | 35 | - |
- |
Klebsiella pneumoniae |
| 3.5.5.1 | 35 | - |
strain PA-34 | Rhodococcus rhodochrous |
| 3.5.5.1 | 40 | 45 | strain ATCC 8750 | Alcaligenes faecalis |
| 3.5.5.1 | 40 | - |
- |
Fusarium oxysporum |
| 3.5.5.1 | 44 | - |
strain JM3 | Alcaligenes faecalis |
| 3.5.5.1 | 45 | - |
strain J1 | Rhodococcus rhodochrous |
| 3.5.5.1 | 50 | - |
- |
Acinetobacter sp. |
| 3.5.5.1 | 55 | - |
- |
Rhodococcus rhodochrous |
| 3.5.5.1 | 65 | - |
- |
Aeribacillus pallidus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 5.5 | - |
- |
Rhodococcus rhodochrous |
| 3.5.5.1 | 6 | 9 | - |
Aeribacillus pallidus |
| 3.5.5.1 | 6 | 11 | - |
Fusarium oxysporum |
| 3.5.5.1 | 7 | 9.5 | strain NCIB11215 | Nocardia sp. |
| 3.5.5.1 | 7.5 | - |
- |
Rhodococcus sp. |
| 3.5.5.1 | 7.5 | - |
strain PA-34 | Rhodococcus rhodochrous |
| 3.5.5.1 | 7.5 | - |
strains ATCC 8750 and JM3 | Alcaligenes faecalis |
| 3.5.5.1 | 7.6 | - |
strain J1 | Rhodococcus rhodochrous |
| 3.5.5.1 | 7.8 | 9.1 | - |
Fusarium solani |
| 3.5.5.1 | 8 | - |
- |
Acinetobacter sp. |
| 3.5.5.1 | 8 | - |
strain NCIB11216 | Nocardia sp. |
| 3.5.5.1 | 9.2 | - |
- |
Klebsiella pneumoniae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.66 | additional information | - |
broad range | Stemphylium loti |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
