Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Miyairi, K.; Ogasawara, A.; Tonouchi, A.; Hosaka, K.; Kudou, M.; Okuno, T.
    Low-molecular-weight pectate lyase from Streptomyces thermocarboxydus (2004), J. Appl. Glycosci., 51, 1-7.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ maximal activity at 0.6 mM Streptomyces thermocarboxydus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 28000
-
 x * 28000, SDS-PAGE Streptomyces thermocarboxydus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Streptomyces thermocarboxydus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2 pectate lyase I Streptomyces thermocarboxydus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 oligogalacturonic acid the enzyme acts on polygalacturonic acids and oligogalacturonic acids over digalacturonic acid and better on the larger galacturonic acids until at least DP8 Streptomyces thermocarboxydus ?
-
 ?
4.2.2.2 pectin better substrate for pectate lyase I than polygalacturonic acid Streptomyces thermocarboxydus ?
-
 ?
4.2.2.2 polygalacturonic acid endo-type reaction, little action on highly esterified polygalacturonic acid methylglucoside.The enzyme acts on polygalacturonic acids and oligogalacturonic acids over digalacturonic acid and better on the larger galacturonic acids until at least DP8 Streptomyces thermocarboxydus 4,5-unsaturated digalacturonic acid + 4,5-unsaturated trigalacturonic acid + oligogalacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 28000, SDS-PAGE Streptomyces thermocarboxydus

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 pectate lyase I
-
 Streptomyces thermocarboxydus
4.2.2.2 PL I
-
 Streptomyces thermocarboxydus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 50
-
 stable up to Streptomyces thermocarboxydus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 9
-
-
 Streptomyces thermocarboxydus