Purification and characterization of pectate lyase I and II from fungus Stereum purpureum
Miyairi, K.; Nishida, K.; Takarae, M.; Shikanai, Y.; Okuno, T.; J. Appl. Glycosci. 49, 99-106 (2002)
No PubMed abstract available
Data extracted from this reference:
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.2.2
Ca2+
both pectate lyase I and II require 0.2 mM Ca2+ for maximal activity
Chondrostereum purpureum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.2.2
48000
-
x * 48000, SDS-PAGE
Chondrostereum purpureum
Organism
EC Number
Organism
UniProt
Commentary
Textmining
4.2.2.2
Chondrostereum purpureum
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
4.2.2.2
glycoprotein
-
Chondrostereum purpureum
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
4.2.2.2
pectate lyase I and II
Chondrostereum purpureum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.2.2
culture filtrate
-
Chondrostereum purpureum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4.2.2.2
polygalacturonic acid
endo-type reaction
651603
Chondrostereum purpureum
4,5-unsaturated digalacturonic acid + 4,5-unsaturated trigalacturonic acid + oligogalacturonic acid
-
651603
Chondrostereum purpureum
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.2.2
?
x * 48000, SDS-PAGE
Chondrostereum purpureum
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.2.2
50
-
stable up to
Chondrostereum purpureum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.2.2
9.5
-
-
Chondrostereum purpureum
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.2.2
Chondrostereum purpureum
pectate lyase II
-
4.1
4.2.2.2
Chondrostereum purpureum
pectate lyase I
-
4.2
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.2.2
Ca2+
both pectate lyase I and II require 0.2 mM Ca2+ for maximal activity
Chondrostereum purpureum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.2.2
48000
-
x * 48000, SDS-PAGE
Chondrostereum purpureum
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
4.2.2.2
glycoprotein
-
Chondrostereum purpureum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.2.2
pectate lyase I and II
Chondrostereum purpureum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.2.2
culture filtrate
-
Chondrostereum purpureum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4.2.2.2
polygalacturonic acid
endo-type reaction
651603
Chondrostereum purpureum
4,5-unsaturated digalacturonic acid + 4,5-unsaturated trigalacturonic acid + oligogalacturonic acid
-
651603
Chondrostereum purpureum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.2.2
?
x * 48000, SDS-PAGE
Chondrostereum purpureum
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.2.2
50
-
stable up to
Chondrostereum purpureum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.2.2
9.5
-
-
Chondrostereum purpureum
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.2.2
Chondrostereum purpureum
pectate lyase II
-
4.1
4.2.2.2
Chondrostereum purpureum
pectate lyase I
-
4.2