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Literature summary extracted from
- Charge optimization increases the potency and selectivity of a chorismate mutase inhibitor (2003), J. Am. Chem. Soc., 125, 5598-5599.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | overexpression in Escherichia coli | Bacillus subtilis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.5 | DELTA117-127 | lower turnover and lower KM than wild-type enzyme | Bacillus subtilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.5 | (1R,3R,5S)-3-carboxy-1-hydroxy-2-oxabicyclo[3.3.1]non-6-ene-5-carboxylate | - |
Bacillus subtilis |  |
| 5.4.99.5 | (1R,3R,5S)-3-carboxy-1-hydroxy-2-oxabicyclo[3.3.1]non-6-ene-5-carboxylate | - |
Escherichia coli | |
| 5.4.99.5 | (1S,3R,5R)-1-hydroxy-5-nitro-2-oxabicyclo[3.3.1]non-6-ene-3-carboxylic acid | - |
Bacillus subtilis | |
| 5.4.99.5 | (1S,3R,5R)-1-hydroxy-5-nitro-2-oxabicyclo[3.3.1]non-6-ene-3-carboxylic acid | - |
Escherichia coli | |
| 5.4.99.5 | (1S,3S,5R)-1-hydroxy-5-nitro-2-oxabicyclo[3.3.1]non-6-ene-3-carboxylic acid | - |
Bacillus subtilis | |
| 5.4.99.5 | (1S,3S,5R)-1-hydroxy-5-nitro-2-oxabicyclo[3.3.1]non-6-ene-3-carboxylic acid | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 0.074 | - |
chorismate | wild-type | Bacillus subtilis | |
| 5.4.99.5 | 0.39 | - |
chorismate | chorismate mutase domain of P-protein | Escherichia coli | |
| 5.4.99.5 | 9.6 | - |
chorismate | DELTA 117-127 | Bacillus subtilis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.5 | Chorismate | Bacillus subtilis | shikimate pathway in bacteria, fungi and higher plants that leads to tyrosine and phenylalanine | Prephenate | - |
? | |
| 5.4.99.5 | Chorismate | Escherichia coli | shikimate pathway in bacteria, fungi and higher plants that leads to tyrosine and phenylalanine | Prephenate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.5 | Bacillus subtilis | - |
- |
- |
| 5.4.99.5 | Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.5 | Chorismate | - |
Bacillus subtilis | Prephenate | - |
? | |
| 5.4.99.5 | Chorismate | - |
Escherichia coli | Prephenate | - |
? | |
| 5.4.99.5 | Chorismate | shikimate pathway in bacteria, fungi and higher plants that leads to tyrosine and phenylalanine | Bacillus subtilis | Prephenate | - |
? | |
| 5.4.99.5 | Chorismate | shikimate pathway in bacteria, fungi and higher plants that leads to tyrosine and phenylalanine | Escherichia coli | Prephenate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.5 | BsCM | - |
Bacillus subtilis |
| 5.4.99.5 | chorismate mutase | - |
Bacillus subtilis |
| 5.4.99.5 | chorismate mutase | - |
Escherichia coli |
| 5.4.99.5 | EcCM | - |
Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 2 | 8 | chorismate | mutant DELTA117-127 | Bacillus subtilis | |
| 5.4.99.5 | 41 | - |
chorismate | wild-type | Bacillus subtilis | |
| 5.4.99.5 | 64 | - |
chorismate | chorismate mutase domain of P-protein | Escherichia coli | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 0.00032 | - |
(1S,3R,5R)-1-hydroxy-5-nitro-2-oxabicyclo[3.3.1]non-6-ene-3-carboxylic acid | 30°C, pH 7.5, wild-type | Bacillus subtilis | |
| 5.4.99.5 | 0.001 | - |
(1R,3R,5S)-3-carboxy-1-hydroxy-2-oxabicyclo[3.3.1]non-6-ene-5-carboxylate | 30°C, pH 7.5, wild-type | Bacillus subtilis | |
| 5.4.99.5 | 0.0023 | - |
(1R,3R,5S)-3-carboxy-1-hydroxy-2-oxabicyclo[3.3.1]non-6-ene-5-carboxylate | 30°C, pH 7.5, chorismate mutase domain of P-protein | Escherichia coli | |
| 5.4.99.5 | 0.0029 | - |
(1S,3R,5R)-1-hydroxy-5-nitro-2-oxabicyclo[3.3.1]non-6-ene-3-carboxylic acid | 30°C, pH 7.5, mutant DELTA117-127 | Bacillus subtilis | |
| 5.4.99.5 | 0.0076 | - |
(1S,3R,5R)-1-hydroxy-5-nitro-2-oxabicyclo[3.3.1]non-6-ene-3-carboxylic acid | 30°C, pH 7.5, chorismate mutase domain of P-protein | Escherichia coli | |
| 5.4.99.5 | 0.051 | - |
(1R,3R,5S)-3-carboxy-1-hydroxy-2-oxabicyclo[3.3.1]non-6-ene-5-carboxylate | 30°C, pH 7.5, mutant DELTA117-127 | Bacillus subtilis | |
| 5.4.99.5 | 0.23 | - |
(1S,3S,5R)-1-hydroxy-5-nitro-2-oxabicyclo[3.3.1]non-6-ene-3-carboxylic acid | 30°C, pH 7.5, wild-type | Bacillus subtilis | |
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