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Literature summary extracted from

  • Reinhardt, L.A.; Svedruzic, D.; Chang, C.H.; Cleland, W.W.; Richards, N.G.J.
    Heavy atom isotope effects on the reaction catalyzed by the oxalate decarboxylase from Bacillus subtilis (2003), J. Am. Chem. Soc., 125, 1244-1252.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.2 O2 requires catalytic dioxygen for activity Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.2 yvrk gene, expression in Escherichia coli BL21(DE3) Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.2
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.2 additional information
-
 additional information kinetic data, kinetic mechanism Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.2 Mn2+ contains Mn2+ in its resting state and two Mn-binding sites, may be only the C-terminal binding site is catalytically active, the N-terminal site not, manganese in the active site can abstract an electron from bound substrate Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.2 oxalate + H+ Bacillus subtilis
-
 formate + CO2
-
 ?
4.1.1.2 oxalate + H+ Bacillus subtilis 168
-
 formate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.2 Bacillus subtilis
-
-
-
4.1.1.2 Bacillus subtilis 168
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.2 recombinant OXDC Bacillus subtilis

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.2 oxalate = formate + CO2 detailed catalytic mechanism, kinetic mechanism Bacillus subtilis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.2 additional information
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.2 oxalate + H+
-
 Bacillus subtilis formate + CO2
-
 ?
4.1.1.2 oxalate + H+ mechanism, multistep model in which a reversible, proton-coupled, electron transfer from bound oxalate to the Mn-enzyme gives an oxalate radical, which decarboxylates to yield a formate radical anion, subsequent reduction and protonation of this intermediate then gives formate, irreversible decarboxylation step, no net redox change between substrate and products, roles of Arg-270 and Glu-333 in catalysis Bacillus subtilis formate + CO2
-
 ?
4.1.1.2 oxalate + H+
-
 Bacillus subtilis 168 formate + CO2
-
 ?
4.1.1.2 oxalate + H+ mechanism, multistep model in which a reversible, proton-coupled, electron transfer from bound oxalate to the Mn-enzyme gives an oxalate radical, which decarboxylates to yield a formate radical anion, subsequent reduction and protonation of this intermediate then gives formate, irreversible decarboxylation step, no net redox change between substrate and products, roles of Arg-270 and Glu-333 in catalysis Bacillus subtilis 168 formate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.2 More member of the cupin superfamily Bacillus subtilis
4.1.1.2 OXDC
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.2 21 22 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.2 additional information
-
 pH-dependence of OxDC activity Bacillus subtilis