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Literature summary extracted from

  • Romagni, J.G.; Dayan, F.E.
    Measuring asparagine synthetase activity in crude plant extracts (2000), J. Agric. Food Chem., 48, 1692-1696.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.4 glycerol required for optimum activity Pisum sativum
6.3.5.4 glycerol required for optimum activity Glycine max
6.3.5.4 glycerol required for optimum activity Lupinus albus
6.3.5.4 additional information 1 mM aminooxyacetic acid increases AS activity in crude extract by inhibiting various aminotransferases and preventing the assimilation of aspartate into the TCA cycle Pisum sativum

General Stability

EC Number General Stability Organism
6.3.5.4 unstable enzyme, 20-25% v/v glycerol and 65-70 mM 2-mercaptoethanol stabilize during storage at low temperatures Pisum sativum
6.3.5.4 unstable enzyme, 20-25% v/v glycerol and 65-70 mM 2-mercaptoethanol stabilize during storage at low temperatures Glycine max
6.3.5.4 unstable enzyme, 20-25% v/v glycerol and 65-70 mM 2-mercaptoethanol stabilize during storage at low temperatures Lupinus albus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 L-asparagine product inhibition Lupinus albus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.4 additional information Pisum sativum primary enzyme responsible for asparagine synthesis ?
-
 ?
6.3.5.4 additional information Glycine max primary enzyme responsible for asparagine synthesis ?
-
 ?
6.3.5.4 additional information Lupinus albus primary enzyme responsible for asparagine synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Glycine max
-
 Merr. v. DP3588
-
6.3.5.4 Lupinus albus
-
 v. Victoria
-
6.3.5.4 Pisum sativum
-
 v. Little Marvel, dark-grown
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 cotyledon etiolated Glycine max
-
6.3.5.4 cotyledon etiolated Lupinus albus
-
6.3.5.4 leaf first leaves, etiolated Pisum sativum
-

Storage Stability

EC Number Storage Stability Organism
6.3.5.4 -25°C, semicrude extract, 9-10 days, 50% loss of activity Lupinus albus
6.3.5.4 -80°C, semicrude extract, over 3 weeks, 30% loss of activity Lupinus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine ATP-dependent, the amine group of Gln is transferred directly to Asp Pisum sativum AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 ATP + L-aspartate + L-glutamine ATP-dependent, the amine group of Gln is transferred directly to Asp Glycine max AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 ATP + L-aspartate + L-glutamine ATP-dependent, the amine group of Gln is transferred directly to Asp, maximum activity with 1 mM Gln and 3-10 mM ATP in the assay Lupinus albus AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 additional information primary enzyme responsible for asparagine synthesis Pisum sativum ?
-
 ?
6.3.5.4 additional information primary enzyme responsible for asparagine synthesis Glycine max ?
-
 ?
6.3.5.4 additional information primary enzyme responsible for asparagine synthesis Lupinus albus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.4 AS
-
 Pisum sativum
6.3.5.4 AS
-
 Glycine max
6.3.5.4 AS
-
 Lupinus albus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.5.4 25
-
 assay at Pisum sativum
6.3.5.4 25
-
 assay at Glycine max
6.3.5.4 25
-
 assay at Lupinus albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.4 7.5
-
 assay at Pisum sativum
6.3.5.4 7.5
-
 assay at Glycine max
6.3.5.4 7.5
-
 assay at Lupinus albus